You are running your first ELISA and make some mistakes. What would happen if each of the following happened in isolation? Would the mistake inherently make the data unusable? (Tip: You might find the trouble shooting table from an ELISA protocol helpful, one is posted to ICON) a) You only add 50ul instead of 100 ul of both standards and experimental samples b) You use the same pipette tips to add the HRP-detection antibody and TMB substrate. c) You forget about your plate after adding your sample to a pre-coated plate and it incubates for an extra 4 hours. d) You forget about your plate after adding the TMB substrate and it incubates for an extra 4 hours. e) After adding the HRP-Detection antibody, you are so excited to see the result, you decide to wash just once and then add the TMB reagent.
You are running your first ELISA and make some mistakes. What would happen if each of the following happened in isolation? Would the mistake inherently make the data unusable? (Tip: You might find the trouble shooting table from an ELISA protocol helpful, one is posted to ICON) a) You only add 50ul instead of 100 ul of both standards and experimental samples b) You use the same pipette tips to add the HRP-detection antibody and TMB substrate. c) You forget about your plate after adding your sample to a pre-coated plate and it incubates for an extra 4 hours. d) You forget about your plate after adding the TMB substrate and it incubates for an extra 4 hours. e) After adding the HRP-Detection antibody, you are so excited to see the result, you decide to wash just once and then add the TMB reagent.
Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter6: Basic Clinical Chemistry
Section6.8: Fecal Occult Blood Test
Problem 10RQ
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Transcribed Image Text:You are running your first ELISA and make some mistakes. What would happen if each
of the following happened in isolation? Would the mistake inherently make the data
unusable? (Tip: You might find the trouble shooting table from an ELISA protocol
helpful, one is posted to ICON)
a) You only add 50ul instead of 100 ul of both standards and experimental
samples
b) You use the same pipette tips to add the HRP-detection antibody and
TMB substrate.
c) You forget about your plate after adding your sample to a pre-coated plate
and it incubates for an extra 4 hours.
d) You forget about your plate after adding the TMB substrate and it
incubates for an extra 4 hours.
e) After adding the HRP-Detection antibody, you are so excited to see the
result, you decide to wash just once and then add the TMB reagent.
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