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A: ELISA stands for enzyme linked immunosorbent assay. It is used for identifying antigen.
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Q: what is the principle of ELISA? What is the procedure of direct and indirect ELISA and what is the…
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A: Answer
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Q: Why do you think it is called indirect ELISA?
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Q: State two reasons behind using the blocking buffer in ELISA experiment.
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Q: In five sentences, summarize how an ELISA assay “works.” How is the test quantified?
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What are the four steps of an Elisa protocol
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- Write down basic principle of ELISA and its main steps? Write names of different types of ELISA. (Subject: Biotechnology)ELISA tests usually use a primary and secondary antibody. Why? What are the necessary controls one would need to perform to ensure that the antibody specificities are valid (i.e., no false-positive or falsenegative reactions)?What is the label used in ELISA? Can we use different label? Why, or why not.