a) did the positive and negative controls work correctly in this ELISA? Explain. B) in the sample wells( wells3-7) some are darker blue than others. Explain what happened here and how you'd interpret the results?
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a) did the positive and negative controls work correctly in this ELISA? Explain.
B) in the sample wells( wells3-7) some are darker blue than others. Explain what happened here and how you'd interpret the results?
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- Place the steps in the correct order to assess your understanding of the indirect ELISA. Indirect USA Enzyme-linked antibody adkued to wel. Well A WellB Rinse wels. Add colorless substrate. Add known antigen Add sorum sample from patent. Color develops if HIV antibodies were present In serum Rinse well to remove unbound antbodies Reset Copyright CMcaraw-Hil Education, Permission required for reproduction or display. < Prev 23 of 44 NextFigure 1. Cytotoxicity test on S96 well plates that was monitored by ELISA reader 3- Discussion 1- Explain another methods by which cytotoxicity is measured ?You are running an ELISA to test three patients for COVID-19.The results of the test are shown below (and in the PDF).What statement is TRUE based on the results shown? Patient A 1:2 diluton 1:10 diluton 1:100 diluton Patient B Negative Control Positive Control Patient C 888 k. Patient A has COVID-19. 1. Patient B has COVID-19. m. Patient C has COVID-19. n. Patients B and C have COVID-19. o. Patients A and B have COVID-19.
- If the test samples are positive for the antigen in the ELISA we are using, what color will you see? Blue Pink Green O Orange1. Sensitivity, Specificity and Predictive Values The table below shows the results from looking at the diagnostic accuracy of a new rapid test for HIV in 2,387subjects, compared to the Reference standard ELISA test. The rows of the table represent the test result and the columns the true disease status (as confirmed by ELISA). ELISA test New Rapid test HIV (+) HIV (-) Test + 165 562 Test- 25 1635 Complete this Table: Solution Final answer (Round off up to 2 decimal places) :( 1 pt) Items (no short cut ):(2 pts) Specificity of the New Rapid test For HIV Sensitivity of the New Rapid test For HIV Positive Predictive Value of the new rapid test for HIVV Negative Predictive Value of the new rapid test for HIV Interpret the computed values. (2 pts each)The direct ELISA test requires only one antibody only one antigen both primary and secondary antibody only secondary antibody conjugated with enzyme system
- 29. Correctly label each image below with the ELISA method it is describing (3pts) Sand Ag Substrate ghta feel 30SICA S Primary Antibody Conjugate Substrate Secondary Antibody Conjugate You Substrate Capture Antibody Seeslo aidi onirif gnil 30. Andrew is a doctor in a hospital. He is treating a patient with a bacterial respiratory infection. He wants to know what the best antibiotic to prescribe for this infection is, so isolates the microorganism and does a Kirby-Bauer test. His results are pictured below. (3pts)Below is a picture of ELISA results. Why is the color darker in some wells and lighter in others? Assume that all of these wells are test wells, not controls.After centrifugation, the serum had a noticeable red/pink hue. 1. What is the appropriate next action for the medical technologist? 2. How will this affect each parameter to be tested?
- 1. Is it really necessary to add substrate in an ELISA test? Explain why, or why not. 2. What is the label used in ELISA? Can different labels be used? Justify your answer. 3. Explain the positive and negative results of an ELISA test. 4. Why do you think indirect ELISA is called "indirect"? 5. What are the different solid phases that antibody or antigen can bind to, in ELISA? 6. What is the importance of washing in ELISA? When is washing performed? 7. Assuming that these are the 12 microplates. What is wrong with the result of the test? What do you think the cause of this occurrence? What should the medical technologist do? 1000000000 GD GD GD AA AA for number 7what is and how do you preform a MR test aka Methyl red testWhen an enzyme-linked antibody is added in an ELISA test (select all appropriate), Group of answer choices A. it will be washed away if the plasma sample is positive for the substance we are testing for B. It will attach to the substance we are testing for if the sample is positive C. it will stick if the plasma sample is positive for the substance we are testing for