Write out the mechanism of TIM-catalyzed reaction in the direction it goes in glycolysis. Show involved enzymatic residues.
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- 1. What are the effects of pH and temperature to catalase? What is the optimum pH and optimum temperature for catalase? 2. Explain why the rate of reaction initially increases with increase in temperature then gradually declines as the temperature is further increased. 3. Is the rate of enzymatic reaction always directly dependent on enzyme concentration? Explain. 4. Explain the effect of substrate concentration on enzyme activity. 5. What is the effect of CuSO, on the enzymatic activity of catalase? 6. Is CuSO4 an activator or inhibitor? If it is an inhibitor, what kind of inhibitor is it?28. Sucrose in human nutrition is hydrolyzed into its monosaccharide components by sucrase. Glucose can enter directly in cellular respiration while fructose is predominantly metabolized in the liver as illustrated in the figure below. Suppose 180 molecules of sucrose are to be catabolized, what is the net ATP production during glycolysis considering both substrate-level phosphorylation and oxidative phosphorylation of NADH shuttled by malate and aspartate to the mitochondrion? [Hint: Sum of net ATP yield in substrate-level phosphorylation and ATP from glycolytic NADH] Fructose Glucose Glycogen ATP- ATP- ADP4 ADP+ hexokinase glucokinase Glucose-6-P→→→→→ Glucose-1-P Fructose-6-P Fructose-1,6-BP fructokinase rapid Fructose-1-P Rate aldolase B limiting Dihydroxyacetone-P Glyceraldehyde ATP- triose kinase ADP Glyceraldehyde-3-P Liver Also intestinal cells and kidney cortex O a. 810 ATP O b. 1260 ATP O c. 1800 ATP d. 2520 ATP PFK-1 Dihydroxy- acetone-P Lactate F1,6bisphosphatase…5. The isomerization of dihydroxyacetone phosphate to glyceraldehyde-3-phosphate is necessary so that the two molecules generated from fructose-1,6-bisphosphate can proceed through the remainder of the glycolysis pathway. Draw a mechanism for this interconversion using generic acid/base catalysts. E-E CH,OH CHO НС—ОН CH2OPO,2- ČH2OPO,2-
- 1. Write the ten reactions of the glycolytic pathway, naming all enzymes and providing the structural formulas of their substrates and products.3. Answer the following questions about the metabolic pathway shown below: glutamate dehydrogenase e NH3 0-C-C-cH2-CH2-C- 0-C-CH-CH2-CH2-C-O + H,O + NAD + NH + NADH + H (a) Label the correct substances as the substrate, enzyme, and co-enzyme. (b) Which of the six classes does the enzyme of this reaction belong to? Why? (c) What is the name of the first molecule in this reaction? (d) Which metabolic pathway is this reaction likely to be a part of? A. glycolysis B. deamination C. beta-oxidation D. fermentation9) After the run, what happens to the glucose under anaerobic conditions? Explain, (think the cori cycle)
- 10. Glycogen, as the main storage form of glucosc, is an important energy reservoir. Describe the role of glycogen in providing the body with energy. To answer: a) write a scheme for glycogen mobilization, and oxidation of the end product to CO, and H,O; b) mark the reactions associated with ATP synthesis and ATP consumption in the scheme, calculate the oxidation energy yield of I mole of the final product resulting from glycogen breakdown; 1L An unconscious man with sions of alcobol poisonino was taken to the hosnital1. The first step in the payoff phase of glycolysis is catalyzed by the enzyme glyceraldehyde 3-phosphate dehydrogenase, an enzyme that contains a nucleophilic cysteine playing a central role in the reaction. A) In the direction of gluconeogenesis, what reaction does this enzyme catalyze? AG° = -6.3 kcal/mol for this reaction in the direction of gluconeogenesis. Based on what you know about the substrates involved, provide two chemical reasons as to why the AGO of this reaction is negative.10. General equation of anaerobic oxidation of glucose.
- a) Describe the 10 enzymatic reactions of glycolysis and the organization of the pathway in 2 main phases.1. Put together the sequence of components (numbers) that form the glycolysis pathway and the linked reaction. Choose the sequence from the following metabolites. Also mention the step in which NADH/FADH2 is formed along with the number. > Acetyl Co.A > Glucose. > Pyruvate. Fructose – 1, 6-bisphosphate > Glucose – 6 – phosphate. > Phosphoenol – pyruvate. > 1, 3 – Bisphosphoglycerate. > Glyceraldehyde 3-phosphate Fructose – 6 – phosphate > Glycerol 2-phosphate /2-phosphoglycerate. > Dihydroacetone – phosphate. > Glycerol – 3 – phosphate / 3- phosphoglycerate.1. What is the common metabolic pathway of these three nutrients? Explain the process involved. 2. Make a flow chart diagram of each step in Glycolysis. Identify the biochemical reactions in each step and its corresponding enzymes and coenzymes. 3. What metabolic products are formed from pyruvate in each case: a) anaerobic conditions in the body b) anaerobic conditions in yeast c) aerobic conditions