You run a series of assays at 25°C on enzyme A. You measure the velocity for a range of S concentrations. What is the Km (in mM) for Enzyme A? [Compound S] Enzyme A velocity(mm)(nmol/min)0.512481632641282562.85.29.114.420.425.829.732.133.534.2

This question can be solved using Lineweaver Burk plot. This graph also represents the enzyme…

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You run a series of assays at 25°C on enzyme A. You measure the velocity for a range of S concentrations. What is the Km (in mM) for Enzyme A?

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

You run a series of assays at 25°C on enzyme A. You measure the velocity for a range of S concentrations. What is the Km (in mM) for Enzyme A?

[Compound S] Enzyme A velocity
(mm)
(nmol/min)
0.5
1
2
4
8
16
32
64
128
256
2.8
5.2
9.1
14.4
20.4
25.8
29.7
32.1
33.5
34.2

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