How can molecular structural analysis be accomplished with electrospray ionization mass spectrometry?
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- What is the purpose of tandem mass spectrometry?Outline the techniques of two-dimensional gel electrophoresisand tandem mass spectroscopy, and explain why they are used.In gel electrophoresis and column chromatography, why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?
- Explain the differences between gel electrophoresis and column chromatography. Address the principles behind each separation. Why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?Can two-dimensional gel electrophoresis be used as a purificationtechnique? Explain.what are the advantages and disadvantages of using recombinant protein and affinity chromatography for protein purification compared to gel filtration (size exclusion chromatography) and DEAE-sepharose chromatography (ion-exchange chromatography)?
- How proteins are analyzed using gel electrophoretic techniques? Write the differences and uses of 1D and 2D polyacrylamide gel electrophoresis techniques.What are the differences among native gel electrophoresis, SDS-PAGE, isoelectric focusing, isotachophoresis, 2-D gel electrophoresis, and pulsed-field gel electrophoresis in terms of denaturants used, pH and electrolyte content, and application of electric field gradient?Explain how charged molecules are separated using gel electrophoresis technique