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Loop with bacterial inoculum was subjected to flame sterilization prior to streaking on agar plate.
Effect:
Rationale:
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- Topic: Preparation of Microbial Suspension A Pure colony is needed for inoculation in sterile water, a small portion of culture media was included. Effect: Rationale:Oven sterilization of aqueous culture medium. Effect: Rationale:Lid of petri dish was left on bench top for a few minutes after pouring of sterile medium. Effect: Rationale:
- Give two reasons for heating the slide after the smear is air dried?Topic: Spread-Plate Method The bacterial suspension was not spread evenly on the surface of the agar.Effect: Rationale:For preparation of a smear on a slide, what is the purpose of heat fixation? What problems can arise when the slide is heated in a flame?
- Cause-and-Effect Analysis: Given the conditions below, describe the effect on the staining procedure and explain briefly: 1. Excessive heat was applied during fixation 2. Low concentration of crystal violet used during gram staining 3. Excessive washing between steps 4. Insufficient decolorization 5. Excessive counterstainingOther than the application of heat, name other methods of sterilization. Define the methods and give examples of materials sterilized in each method.List 5 other methods of sterilization and describe the principle/s and when each method is used. What are the requirements to support microbial growth?
- What is the purpose of flame sterilizing the inoculating loop or needle before and after usingit? What is meant by ‘streak dilution’ technique? What is the purpose of placing the inoculated agar plate in an inverted position in theincubator?procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar plate to agar slant.Compare and contrast antiseptic and disinfectant. What is the hazard of the splattering tendency in flame sterilizing an inoculating loop? Can inoculation loops and needles be sterilized by autoclaving? Explain.