Which one of the following methods of antimicrobial susceptibility testing requires a dilution series of the antibiotic and can provide a minimum inhibitory concentration? Select one: a. Disk diffusion method b. Broth microdilution method c. Agar macrodilution method d. Gradient diffusion method
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- Which one of the following methods of antimicrobial susceptibility testing combines some of the properties of two other methods and involves adding a plastic strip to the agar? Select one: a. Agar macrodilution method b. Gradient diffusion method c. Broth microdilution method d. Disk diffusion methodThe Kirby -Bauer antimicrobial sensitivity testing method relies on: A. The inhibition of one antimicrobial by another B. The diffusion of antimicrobials in paper discs C. Serial dilutions of antimicrobials in test tubes D. A gradient of an antimicrobial on a paper stripBriefly discuss the potential impact on the antibiotic susceptibility testing results when different agar thicknesses are used across different laboratories. What would you suggest to ensure that results between testing laboratories are comparable.
- Discuss the procedure and application for the agar well diffusion method of evaluating antimicrobial activity.What results on a microdilution antimicrobial susceptibility test would indicate an antibiotic would be an effective treatment for an infection? O a. Growth next to the antibiotic O b. High optical density in the well with inoculum and antibiotic O c. Low optical density in the well with inoculum and antibiotic O d. No growth next to the antibiotic disk Clear my choice Next pageEx. 17: Chemotherapeutic Agents Using the Antimicrobial Susceptibility Table on pg 62 in the lab manual, determine whether not the bacteria plated in this Kirby-Bauer test are resistant (R), intermediate (I), or susceptible (S). The bacterial species tested was Escherichia coli (Gram negative). Ciprofloxacin Diameter = 20 Streptomycin Diameter 30 Carbenicillin Diameter 17 + Erythromycin Diameter = 23 Novobiocin Diameter = 0 bacterial growth = disk with antibiotic (see labels) = no growth
- diameter AM=47MM TE= 27 MM both left hand side is ampicillin and right hand side is tetracycline With reference to the table below, determine the antibiotic susceptibilities of different antibiotics against coli and S.aureus. S.aureus Antimicrobial agent Interpretive categories and zone diameter breakpoints, nearest whole mm sensitive Intermediate resistant Tetracycline >19 15-18 <14Neutralization of residual disinfectant could be made by: a. Dilution if the concentration exponent is high b. Dilution if the concentration exponent is low c. Adding specific agent e.g. 3% Tween 80 d. Either a or c e. Either b or cWhich type(s) of medium would be used in each scenario?a. isolating the growth of Streptococcus pyogenes from a patient’s throat swabb. isolating a pathogen from a patient’s clean-catch urine samplec. isolating enteric bacteria such as Escherichia coli from a sample oforganically grown spinach d. maintaining a patient’s nasal swab specimen for further analysis andidentifi cation of possible respiratory syncytial virus (RSV) infection
- The following methods used for sterilizing an antibiotic solution. i. Dry heat sterilization ii. Microfiltration iii. Autoclaving iv. Desiccation Select one: O a. i and ii O b. i and iii O c. ii and iv O d. ii only10. Which one of the following methods of antimicrobial susceptibility testing combines some of the properties of two other methods and involves adding a plastic strip to the agar? a. Gradient diffusion method b. Agar macrodilution method c. Disk diffusion method d. Broth microdilution method 11. Most ATP molecules are produced during which phase of aerobia respiration? a. Fermentation b. Krebs cycle c. Glycolysis d. Electron transport chain 12. The goal of disinfection is to eliminate ____ whereas the goal of sterile technique is to exclude ____ a. bacteria , bacteria and viruses b. pathogens, all microorganisms c. nonpathogenspathogens d. all microorganisms, pathogens 13. Which one of the following statements about archaea is false? Select one: a. The cell walls of archaea contain a thicker layer of peptidoglycan than the cell walls of bacteria. b. Some archaea live in extremely hot environments. c. Archaea are more closely related to eukaryotes than they are to bacteria. d.…What appropriate method should be used in controlling microbial growth in each of the following? 1. Chicken serum 2. Fresh goat milk 3. Nutrient broth 4. Paracetamol 5. Inoculation loob