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- Which is more Suitable for long-term storage: agar slant or plate? Explain why?The following is a Mannitol Salt Agar plate. What does the yellow color on the right side of this plate indicate? TAW ASM's MicrobeLibrary Ⓒ Reynolds tolerance of the salt mannitol metabolism tolerance of the dyes and chemicals in the agar lactose metabolismIf one is trying to determine if a culture is pure or mixed, which medium would you choose? Why? (best answer) agar plate; can compare colony morphology of isolated colonies O agar deep; can determine if pure or mixed based on presence or absence of bubbles and cracks O broth tube; can determine pure or mix based on presence or absence of pellicle, turbidity and sediment
- Which of the following is the proper technique for inoculating an agar slant with a broth culture? A. Stab the butt of the media with the wire loop. B. Stab the surface of the agar media with the wire loop beginning at the base of the tube moving toward the mouth as you withdraw the loop. C. Gently move the wire loop back and forth across the surface of the agar beginning at the mouth of the tube moving down toward the base of the slant. D. Gently move the wire loop back and forth across the surface of the agar beginning at the base of the slant as you withdraw it from the tube.If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock solution would you add to 125 ml of medium? how to calculate these kind of question in tissue culture media preperationWhat is the major difference between Thayer-Martin and chocolate agar? When would you use Thayer-Martin rather than chocolate agar?
- Pattern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface area of agar that can be inoculated.O The following is a Mannitol Salt Agar plate. What does the growth on this plate indicate? www TAN ASM's MicrobeLibrary Reynolds lactose metabolism tolerance of the dyes and chemicals in the agar tolerance of the salt mannitol metabolismIs the mannitol salt agar (MSA) a complex or defined medium? Explain based on Composition. What kind of media based on what kind of microorganisms it allows to grow
- A sample of Earth Alive Soil Activator was diluted by transferring 1 gram into a 9 ml dilution blank (tube A) and then serially diluted five (5) more times by transferring 1 ml of a previous dilution into a tube of 9 ml of sterile water. After making these serial dilutions, 2 ml samples were taken from tubes D, E, and F, added into empty petri dishes, and molten PCA was poured into each petri dish. Tubes A, B, and C were heated in a 80˚C water bath for 30 minutes, 2 ml samples of these tubes were added into empty petri dishes, and molten PCA was poured into each petri dish. These plates were allowed to solidify, and they were incubated at 35˚C for 48 hours. After the incubation period, you counted CFUs on all 6 plates. The results of your CFU counts are contained in table 1. Table 1. CFU counts for 3 plates from the heated dilutions (A-C) and the 3 plates from the non-heated dilutions (D-F) # CFUs Not heated Heated A -- 400 B -- 45 C -- 6 D 350 -- E 31 -- F 2 --…In this step, wash the cells with ice-cold PBS. Drain the PBS, then add ice-cold lysis buffer. Scrape adherent cells off the dish using a cold plastic cell scraper, then gently transfer the cell suspension into a pre-cooled microfuge tube. Maintain constant agitation for 30 minutes at 4°C. Centrifuge in a microcentrifuge at 4°C. You may have to vary the centrifugation force and time depending on the cell type. Gently remove the tubes from the centrifuge and place on ice, aspirate the supernatant and place in a fresh tube kept on ice, and discard the pellet. Q3: To make sure that you used a similar amount of samples, what important step should be done before proceeding the electrophoresis stage? Once you have determined the concentration of each sample, you can freeze them at -20°C or -80°C for later use or prepare for immunoprecipitation or for loading onto a gel. Q4. Why is it necessary to store the prepared lysates in a very low temperature? PLEASE ANSWER Q3 AND Q4Create a graph of this spectrophotometric data (using Excel or the graph paper below), labeling the four stages of the growth curve- lag, log (exponential), stationary, death. Be sure to label your axes properly and give your graph a title Time Culture Sampled % Absorbance 0 min 5% 30min 7% 60min 9% 90min 15% 120min 25% 150min 37% 180min 49% 210min 50% 240min 49% 270min…