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Distances traveled by the solvent is 3.2 First spot ( fluorene) is 2.5 Second spot distance equals 1.4 How can we calculate the Rf for the Chromatography experiment?

Distances traveled by the solvent is 3.2 First spot ( fluorene) is 2.5 Second spot distance equals 1.4 How can we calculate the Rf for the Chromatography experiment?

EBK A SMALL SCALE APPROACH TO ORGANIC L
EBK A SMALL SCALE APPROACH TO ORGANIC L
4th Edition
ISBN:9781305446021
Author:Lampman
Publisher:Lampman
Chapter89: Thin-layer Chromatography
Section: Chapter Questions
Problem 1P
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Distances traveled by the solvent is 3.2 First spot ( fluorene) is 2.5 Second spot distance equals 1.4 How can we calculate the Rf for the Chromatography experiment?
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● Purpose: We will separate a ketone (Fluorenone)-hydrocarbone(Flourene) mixture by absorption column chromatography and then check the efficiency of our separation by TLC. Determine the relative % of fluorene and fluorenone in your mixture. ● Column Chromatography Procedure: 1. Obtain column 2. Cover the bottom of the column with cotton wool. 3. Add 1 cm of sand on the top of cotton wool. 4. 5. 6. 7. Add 10g of alumina through a funnel and settle it horizontally on top of the sand. , 62 9 Aluming! 3д тіл Then add 25ml of pet ether Once again add 1cm of sand on top of alumina. Pour solvent on the top of alumina being careful don't let column run dry. (stop it ideally at the sand top) 8. Adding mixture: Dissolve 0.4g of fluorene/ fluorenone mixture in Iml of dicholoromethane () and apply it on the top of the sand. Now, open stopcock and let the liquid flow on through the Alumina. 9 Sample 8. beaker 150mL 66.008g (pre weigh). tare Separation: Add 70 ml of 2:1 pet ether: dichloromethane. Repeat this elution and collect the second 70 ml of eluent in flask #2. Add 70 ml of dichloromethane to the column and repeat this process to fill flask #3 with 70 ml of eluent. Now, you should have three flasks of solvent. Transfer each of these cluents into three pre-weighed beakers and place them over a steam bath. Determine the mass of three products. TLC procedure: Place a piece filter paper into the developing chamber, this helps TLC plate to remain in the aqueous mobile phase. Draw a line 2 cm away from the bottom of the plate and spot eluted compounds' samples on it by using capillary tubes, it is called origin line. ● Place the plate into a chamber and use pet ether/ dichloromethane 1:1 as eluting solvent. • Remove the plate from chamber and mark the solvent front with a pencil. Observe the plate under the UV lamp and mark the spots with pencils. Calculate values. Determine if products are pure and perform TLC with pure samples of fluorine and 9-fluorenone. Fill the column with 70 ml of pet ether and open the stopcock, collect 70 ml eluent into Erlenmeyer flask #1.
Transcribed Image Text:● Purpose: We will separate a ketone (Fluorenone)-hydrocarbone(Flourene) mixture by absorption column chromatography and then check the efficiency of our separation by TLC. Determine the relative % of fluorene and fluorenone in your mixture. ● Column Chromatography Procedure: 1. Obtain column 2. Cover the bottom of the column with cotton wool. 3. Add 1 cm of sand on the top of cotton wool. 4. 5. 6. 7. Add 10g of alumina through a funnel and settle it horizontally on top of the sand. , 62 9 Aluming! 3д тіл Then add 25ml of pet ether Once again add 1cm of sand on top of alumina. Pour solvent on the top of alumina being careful don't let column run dry. (stop it ideally at the sand top) 8. Adding mixture: Dissolve 0.4g of fluorene/ fluorenone mixture in Iml of dicholoromethane () and apply it on the top of the sand. Now, open stopcock and let the liquid flow on through the Alumina. 9 Sample 8. beaker 150mL 66.008g (pre weigh). tare Separation: Add 70 ml of 2:1 pet ether: dichloromethane. Repeat this elution and collect the second 70 ml of eluent in flask #2. Add 70 ml of dichloromethane to the column and repeat this process to fill flask #3 with 70 ml of eluent. Now, you should have three flasks of solvent. Transfer each of these cluents into three pre-weighed beakers and place them over a steam bath. Determine the mass of three products. TLC procedure: Place a piece filter paper into the developing chamber, this helps TLC plate to remain in the aqueous mobile phase. Draw a line 2 cm away from the bottom of the plate and spot eluted compounds' samples on it by using capillary tubes, it is called origin line. ● Place the plate into a chamber and use pet ether/ dichloromethane 1:1 as eluting solvent. • Remove the plate from chamber and mark the solvent front with a pencil. Observe the plate under the UV lamp and mark the spots with pencils. Calculate values. Determine if products are pure and perform TLC with pure samples of fluorine and 9-fluorenone. Fill the column with 70 ml of pet ether and open the stopcock, collect 70 ml eluent into Erlenmeyer flask #1.
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