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- Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.Qno1: why there is a need of chromatography and chromatography thecniqs. B) when and where we should use normal and reverse phase chromatographyA young researcher set out to develop a method for quantifying the polycyclic aromatic hydrocarbon (PAH) content in a contaminated soil sample using open tubular gas chromatography. During the initial stages of method development, the researcher was unable to separate the early eluting compounds, anthracene and naphthalene, with baseline resolution. Select the possible modifications the researcher could make to improve the resolution. use a column with a smaller inner diameter decrease the column length increase the stationary phase thickness increase the temperature choose a different stationary phase
- A student developed a new dye called Dye C. It was analyzed against other dyes using paper chromatography. A solution of 1:1 ethyl acetate:hexane was used as the eluent. The resulting chromatogram is shown below. What other dye/s are present in dye C? Which dye contains the least number of pigments?1a) Hexane, CH3(CH2)4CH3, is found to have a long retention time when separated from a mixture. A column with a C18 (18-carbon chain) stationary phase and a methanol-water modal phase is used for the separation. Explain why hexane has a long retention time. 1b)You wish to chromatographically seperate a mixtureof the follwing compunds. Which of the two compounds will be most difficult to seperatate from one another? Why?what is chromatography? Explain different types of chromatography and explain how to seperate compounds. indicate stationary phase mobile phase.
- 2-chloropentane, pentanoic acid, cyclopentane, and pentanal are among the chemicals found in a combination. If the mixture underwent paper chromatography with the same solvent system employed in the experiment, arrange the following components in INCREASING Rf.Which statement(s) are correct for gas chromatography? A) the components in a mixture can be identified from their retention time. B) the relative peak areas give the proportions of components in a mixture. C) Calibration curves are used to confirms the concentrations of components in a mixture.Can paper chromatography be used to separate and identify very volatile substances? Why? Please explain comprehensively.
- (5) An ion chromatograph is used for the determination of chloride ions. Seven replicate measurements of low level samples are performed and the results are: 20, 21, 18, 22, 20, 22, and 21 µg/L. What is the method detection limit for chloride?Describe the procedural steps to achieve adequate separation of a homogeneous, liquid mixture containing benzoic acid, n-propylbenzene, and phenol, provided a 4:1 hexane to ethyl acetate solution as the eluent, using Thin Layer Chromatography (TLC).QUESTION 5 In a chromatographic separation using a polar stationary phase and a mobile phase composed of 60% hexane and 40% ethyl acetate, two compounds, benzene (peak A) and toluene (peak B), are eluted. The width of peak A at its base is 1.2 min, the width of peak B at its base is 1.3 min, and the retention time of peak A is 5.2 min and peak B is 3.5 min. What is the resolution (Rs) between these two peaks and is it acceptable? R = 1.36. It is not an acceptable resolution. R = 0.74. It is an acceptable resolution. R = 1.36. It is an acceptable resolution. R = 0.74. It is not an acceptable resolution.