Please provide the best possible work/ answers for the given problem. Attached is the starting bit of what the problem is referencing. This is Biochemistry. Thank you 5. Properties of an Enzyme of Prostaglandin Synthesis Prostaglandins area class of eicosanoids, fatty acid derivatives with a variety of extremelypotent actions on vertebrate tissues. They are responsible for producingfever and inflammation and its associated pain. Prostaglandins are derivedfrom the 20-carbon fatty acid arachidonic acid in a reaction catalyzed bythe enzyme prostaglandin endoperoxide synthase. This enzyme, acyclooxygenase, uses oxygen to convert arachidonic acid to PGG2, theimmediate precursor of many different prostaglandins (prostaglandinsynthesis is described in Chapter 21).(a) The kinetic data given below are for the reaction catalyzed byprostaglandin endoperoxide synthase. Focusing here on the first twocolumns, determine the Vmax and Km of the enzyme.[Arachidonic acid] Rate of formation of PGG₂(MM)(mm/min)0.51.01.52.53.523.532.236.941.844.0Rate of formation of PGG₂with 10 mg/mL ibuprofen(mm/min)16.6725.2530.4937.0438.91(b) Ibuprofen is an inhibitor of prostaglandin endoperoxide synthase. Byinhibiting the synthesis of prostaglandins, ibuprofen reduces inflammationand pain. Using the data in the first and third columns of the table,determine the type of inhibition that ibuprofen exerts on prostaglandinendoperoxide synthase using a Lineweaver-Burke PLOT. [S][S] mM0.511.52.53.5mM/min23.532.236.941.844w/ibumM/min16.6725.2530.3937.0438.911/[S]1/Vow/ibu1/Vo

According to Michaelis - Menton equation velocity of an enzyme increases with substrate…

(a) The kinetic data given below are for the reaction catalyzed by prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the Vmax and Km of the enzyme. [Arachidonic acid] Rate of formation of PGG₂ (MM) (mm/min) 0.5 1.0 1.5 2.5 3.5 23.5 32.2 36.9 41.8 44.0 Rate of formation of PGG₂ with 10 mg/mL ibuprofen (mm/min) 16.67 25.25 30.49 37.04 38.91 (b) Ibuprofen is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting the synthesis of prostaglandins, ibuprofen reduces inflammation and pain. Using the data in the first and third columns of the table, determine the type of inhibition that ibuprofen exerts on prostaglandin endoperoxide synthase using a Lineweaver-Burke PLOT.

(a) The kinetic data given below are for the reaction catalyzed by prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the Vmax and Km of the enzyme. [Arachidonic acid] Rate of formation of PGG₂ (MM) (mm/min) 0.5 1.0 1.5 2.5 3.5 23.5 32.2 36.9 41.8 44.0 Rate of formation of PGG₂ with 10 mg/mL ibuprofen (mm/min) 16.67 25.25 30.49 37.04 38.91 (b) Ibuprofen is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting the synthesis of prostaglandins, ibuprofen reduces inflammation and pain. Using the data in the first and third columns of the table, determine the type of inhibition that ibuprofen exerts on prostaglandin endoperoxide synthase using a Lineweaver-Burke PLOT.

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter25: Nitrogen Acquisition And Amino Acid Metabolism

Section: Chapter Questions

Problem 12P

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Properties of an Enzyme of Prostaglandin Synthesis.Prostaglandins are a class of eicosanoids, fatty acid derivatives with a variety of extremely potent actions on vertebrate tissues. They are re-sponsible for producing fever and inflammation and its associated pain. Prostaglandins are derived from the 20-carbon fatty acid arachidonic acid in a reaction catalyzed by the enzyme prostaglandin en-doperoxide synthase. This enzyme, a cyclooxygenase, uses oxygen to convert arachidonic acid to PGG2, the immediate precursor of many different prostaglandins (prostaglandin synthesis is described in Chapter 21). a)The kinetic data given below are for the reaction catalyzed by prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the Vmax and Km of the enzyme. b)Ibuprofen is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting the synthesis of prostaglandins, ibuprofen reduces inflammation and pain. Using the data in the first and third columns of…
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