6. Fill in the following blanks for how many of the following products would be generated under aerobic conditions from 1 molecule of pyruvate if a competitive inhibitor of succinyl- CoA synthetase was present in the citric acid cycle? (consider only the conversion of pyruvate to Acetyl-CoA and the TCA cycle, do not consider oxidative phosphorylation reactions or reactions beyond the TCA cycle). i. CO, ii. GTP iii. NADH iv. FADH, V. Oxaloacetate

6. Fill in the following blanks for how many of the following products would be generated under aerobic conditions from 1 molecule of pyruvate if a competitive inhibitor of succinyl- CoA synthetase was present in the citric acid cycle? (consider only the conversion of pyruvate to Acetyl-CoA and the TCA cycle, do not consider oxidative phosphorylation reactions or reactions beyond the TCA cycle). i. CO, ii. GTP iii. NADH iv. FADH, V. Oxaloacetate

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter20: Electron Transport And Oxidative Phosphorylation

Section: Chapter Questions

Problem 7P

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Ethanol as a Source of Metabolic Energy (Integrates with Chapters 19 and 20.) Acetate produced in ethanol metabolism can be transformed into acetyl-COA by the acetyl thiokinase reaction: Acetate+ATP+CoASHacetyleCoA+AMP+PPiAcetyle-CoA then can enter the citric acid cycle and undergo oxidation to 2 CO2by this route, assuming oxidative phosphorylation is part of the process? (Assume all reactions prior to acetyl-CoA entering the citric acid cycle occur outside the mitochondrion). Per carbon atom, which is a better metabolic fuel, ethanol or glucose? That is, how many ATP equivalents per carbon atom are generated by combustion of glucose versus ethanol to CO2?
Using the ActiveModel for enoyl-CoA dehydratase, give an example of a case in which conserved residues in slightly different positions can change the catalytic rate of reaction.
1. Consider the oxidation of the fatty acid with the common name arachidic acid. a. Draw the structure of arachidic acid. b. How many turns of the fatty acid oxidation cycle is required for the complete oxidation of arachidic acid? c. How many moles of ATP are formed from one mole of arachidic acid if the acetyl CoA produced go to the citric acid cycle and oxidative phosphorylation? Assume 1 mole of NADH is equivalent to 3 moles ATP and 1 mole FADH2 is equivalent to 2 moles of ATP. Show how you arrived at your answer
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Select all of the following that accurately apply to acyl-CoA synthetase and the transport of acyl-CoA through the carnitine transport system in the liver: 00 ☐☐☐☐ Acyl-CoA is formed when CoA serves as a nucleophile for attack of the fatty acyl-adenylate intermediate Fatty acid carbon skeletons are simultaneously used in the liver for beta oxidation and glucose biosynthesis The carnitine transport system is inhibited in the presence of high amounts of malonyl CoA The carrier protein carnitine replaces CoA in the transport of fatty acyl groups across the inner- mitochondrial membrane and allows acyl-CoA reformation in the matrix Increased need for beta oxidation allows for decreased transport of fatty acids via the carnitine transport system The formation of acyl-CoA by acyl-CoA synthetase involves the use of 2 equivalents of ATP - (1) an ATP used for activation of fatty acyl-adenylate and (2) an equivalent of ATP in the form of pyrophosphate hydrolysis
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Consider the complete oxidation of one mole of simple TAG containing lignoceric acid residues (24:0). 1. For one mole of the fatty acid residue, determine the following: a. ATP yield obtained from the oxidation of acetyl CoA entering the TCA cycle and ETC I b. ATP yield obtained from NADH coming from the complete ß-oxidation of the fatty acid residue c. ATP yield obtained from FADH₂ coming from the complete ß-oxidation of the fatty acid residue d. net ATP yield of ATP in the complete oxidation of the fatty acid residue 2. What is the net ATP yield for the complete oxidation of all the fatty acid residues of the simple TAG? (Note: glycerol backbone is not included) REMINDER: Answer the following items using numerical value only (e.g. 1, not "1 ATP")
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19) Consider the table of allosteric effectors and their effect on the following metabolic processes. Glycolysis Gluconeogenesis PDH complex Krebs cycle acetyl-CoA Not applicable AMP Not applicable + glucagon Not applicable Not applicable - Each row contains one error. Correct the error in the table below Table 2 Correction (indicate process and effect (+ or -) acetyl-CoA AMP glucagon - Briefly explain the effect of each allosteric effector on the corrected pathway (based on Table 2 above)
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