Q: What is the default state of the lac operon? a. LacI repressor is sitting on the operator sequence…
A: Lacl repressor is sitting on a operator sequence and blocks RNAP from transcribing lacZYA
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Can you please answer number 10 and all of the sub parts
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- 3. Explain why catabolite repression is used in regulating the lac operon and describe how it fine-tunes B-galactosidase synthesis. 4. Compare the control of gene regulation in eukaryotes and prokaryotes in terms of: a. transcription b. regulatory mechanisms and its components28. Which statement does NOT explain why the lac operon would not be effectively regulated by an attenuation mechanism like the trp operon? 1.Lactose is a substrate for the lac operon protein products. 2.Products of lac operon enzymes are not used in protein synthesis. 3.Transcription and translation do not happen simultaneously for the lac operon. 4.Attenuation is a suitable regulatory mechanism for anabolic amino acid pathways. 5.All of these statements explain why regulation by attenuation would not be effective for the lac operon.5. There are four genes in the FAK operon. a. How many mRNAs are encoded by this operon? b. The FAK operon has a weak promoter, where RNA polymerase does not bind well. What type of molecule could directly help RNA polymerase to bind more tightly to this promoter? i. An inducer 6. Consider the Lac operon of E. coli, which is normally expressed only when lactose is present and glucose absent. You identify a mutant strain of bacteria in which Lacz, Lacy, and LacA, are expressed in the absence of glucose, whether or not lactose is present. a. ii. An activator iii. A promoter iv. A repressor v. A ligand C. Assume that one mutation causes this mis-regulation. Which interaction must be disrupted by this mutation? i. Interaction between RNA polymerase and the lac promoter ii. Interaction between Lacl protein and the lac operator (its binding site) Interaction between CRP and CAMP Interaction between CRP-CAMP (aka CAP) its binding site adjacent to lac operon Interaction between Lacl protein…
- 10. Think about the situation where there is an increased concentration of both glucose and allolactose in the media. Does this situation lead to the lac operon activation or not? Justify your answer? 11. Draw the structure of lac operon and its action when the following conditions are prevailing in the media: increased glucose, low glucose, higher c-AMP, low c-AMP, higher glucose, and high c-AMP, low glucose and High c-AMP, Higher glucose and higher allolactose? please assist with the above two questions1. What is the default state of the lac operon? a. LacI repressor is sitting on the operator sequence and blocks RNAP from transcribing lacZYA. b. LacI repressor is not in a correct shape to bind to the operator sequence. c. LacI repressor is not produced in the absence of lactose. d. Allolactose is sitting on the operator sequence and blocks RNAP from transcribing lacZYA.III. Answer the following: 1. What are the main elements of the lac operon and their functions? 2. Distinguish among inducible, repressible, and constitutive gene operons.
- 9. A single base substitution mutation in lacY gene of the Lac operon would render the entire lac operon nonfunctional. True or False.1. Assuming that the level of glucose is low, a mutation in the repressor associated with the lac operon of E. coli which prevents binding of the repressor to lactose should result in: a. constitutive expression of the lac operon genes b. lack of expression or refuced expression of the lac operon genes under all circumstances c. expression of the genes only when lactose is present d. expression of the genes only when lactose is absent e. lack of expression of the lac operon genes, resulting in an increase in lactose production1. Explain why catabolite repression is used in regulating the lac operon anddescribe how it fine-tunes B-galactosidase synthesis. 2.
- 1. Please tell me: What's a mutation in moleculer terms? 2. When a mutation deletes a base in genomic DNA, how does it affect the expression product production and reading frame? 3. How does induction control enzyme synthesis using lactose operon?1. Compare and contrast repressible and inducible operons. 2. Are genes for enzymes involved in anabolic processes usually repressible or inducible , why?1. How does induction control enzyme synthesis using lactose operon? 2. Describe how level of a key metabolite [tryptophane] controls the gene function using tryptophane operon
