10. Modify the diagrams below to reflect the activation and repression of lac operon. Usearrows, lines, X's, etc. to illustrate control of gene expression.a. ActivationlactoseCAPlac lrepressorallolactoseCAMPCRNAPolpromoter operator+1lac Zlac Ylac ADNA lactoseb. RepressionCAPlac IrepressorallolactoseCAMPCRNAPolpromoter operator+1lac Zlac Ylac ADNA

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Description: 10. Modify the diagrams below to reflect the activation and repression of lac operon. Usearrows, lines, X's, etc. to illustrate control of gene expression.a. ActivationlactoseCAPlac lrepressorallolactoseCAMPCRNAPolpromoter operator+1lac Zlac Ylac AD

The prokaryotic gene regulatory system is known as operon system in which the expression of…

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10. Modify the diagrams below to reflect the activation and repression of lac operon. Use arrows, lines, X's, etc. to illustrate control of gene expression. lactose a. Activation CAP lac I repressor allolactose CAMP с RNA Pol promoter operator +1 lac Z lac Y lac A DNA

10. Modify the diagrams below to reflect the activation and repression of lac operon. Use arrows, lines, X's, etc. to illustrate control of gene expression. lactose a. Activation CAP lac I repressor allolactose CAMP с RNA Pol promoter operator +1 lac Z lac Y lac A DNA

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter16: Regulation Of Gene Expression

Section: Chapter Questions

Problem 2TYK: For the E. coli lac operon, when lactose is present: a. and glucose is absent, cAMP binds and...

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For the E. coli lac operon, when lactose is present: a. and glucose is absent, cAMP binds and activates catabolicactivator protein (CAP). b. and glucose is absent, the level of cAMP decreases. c. activated CAP binds the repressor protein to remove it from the operator gene. d. the cell prefers lactose over glucose. e. RNA polymerase cannot bind to the promoter.
10. Modify the diagrams below to reflect the activation and repression of lac operon. Use arrows, lines, X's, etc. to illustrate control of gene expression.S a. Activation lactose lactose CAP lac I repressor allolactose b. Repression CAP allolactose lac I repressor CAMP с CAMP с promoter operator +1 RNA Pol +1 RNA Pol promoter operator lac Z lac Z lac Y lac Y lac A lac A DNA DNA
1. Draw a diagram of the lac operon. PO I+P+O+Z+Y+ I+P+OcZ+Y- I-P+O+Z+Y+ Lab section (circle): lacz gene lac Y gene lacA gene I+P+O+ZY/IPO+Z+Y- 2. The promoter (P) is the start site of transcription through the binding of RNA polymerase before actual mRNA production. Mutated promoters (P) cannot bind RNA polymerase, so no mRNA is made. In the table below, make predictions about the expression of ß-galactosidase and permease for different partial diploid (F') genotypes. Do this for E. coli growth on two different types of media - one without lactose, and one with. Use a to mark where there is no enzyme produced, and a + to mark where the enzyme is made. The first genotype has all wild-type alleles, and has been filled in as an example. Genotype IPOCZ+Y/I-P+O+Z-Y+ ISP+O+Z+Y/I+P+O+Z*Y* CR 1:00 Lactose absent B-galactosidase Permease R 3:-00 + R 6:30 Lactose present B-galactosidase F 10:00 Permease
Answer as Directed. Below is the model of a lac operon. lac I lac Z с promoter operator +1 lac Y lac A DNA 1. In the absence of lactose and the presence of glucose in the bacterial growth media, what proteins are bound to the lac control region? Is the operon being transcribed then? 2. In the presence of lactose and the presence of glucose in the bacterial growth media, what proteins are bound to the lac regulatory region? Is the operon being transcribed then? 3. In the presence of lactose and the absence of glucose in the bacterial growth media, what proteins are bound to the lac control region? 4. Why is it adaptive for a bacterium to not express the genes that encode for that lactose utilization proteins when lactose is not available or when glucose is present? 5. Why is it adaptive for the structural genes for using lactose to be under the control of a single promoter, i.e., synthesize a polycistronic message rather than three monocistronic message?
Answer as Directed. Below is the model of a lac operon. lac I lac Z с promoter operator lac Y lac A DNA +1 1. What are structural genes? Are the lac structural genes transcribed in the absence of lactose? 2. What is the role of the promoter and operator sites in the operon? 3. Is the repressor protein bound to the operator site in the absence of lactose? In its absence? 4. Under what nutritional circumstances (high or low glucose) is CAP bound to cAMP? 5. In the absence of lactose and the presence of glucose in the bacterial growth media, what proteins are bound to the lac control region? Is the operon being transcribed then? 6. In the presence of lactose and the presence of glucose in the bacterial growth media, what proteins are bound to the lac regulatory region? Is the operon being transcribed then? 7. In the presence of lactose and the absence of glucose in the bacterial growth media, what proteins are bound to the lac control region? 8. Why is it adaptive for a bacterium to not…
Read aloud V Draw Highlight 2. You are studying the regulation of the lactose operon in Escherichia coli, by measuring expression of the lacZ gene (i.e production of beta-galactosidase). (a) You identify several loss-of-function mutations in which lacZ is never expressed, in the presence and absence of glucose and lactose. What components of the lac operon could be mutated to produce this phenotype? List all possibilities. (b) You identify another loss-of-function mutation with the following expression pattern: Media + glucose - lactose + glucose +lactose - glucose - lactose - glucose + lactose lacZ expression Low Low High High What components of the lac operon could be mutated to produce this phenotype? List all possibilities.
The molecules indicated below can be used experimentally due to their roles in relation to the expression of the lac operon. Drag the listed molecules to their corresponding bin. Reset Help ONPG IPTG X-Gal allolactose Dragable beta-galactosidase cleaves this molecule to form a visible yellow product. beta-galactosidase cleaves this molecule which then dimerizes to form a visible blue product. This molecule induces the transcription of the lac operon by inactivating the lac repressor.
Like the lac operon, the hexose operon is controlled by a separate regulatory protein under the control of its own promoter (see the schematic of the operon below). The hexose regulatory protein is sensitive to fatty acyl CoA levels. When all hexose fuel sources are depleted, the bacteria switch to lipid metabolism and fatty acyl CoA levels increase. This turns expression of the hexose operon off. +1 +1 Regulatory Gene Pregulator P operon Hexose Operon Genes regulator promoter operon promoter
Part A If E. coll bacteria are placed in an environment that contains both glucose and lactose, the regulation of the lac operon will allow which nutrient to be processed first? ONether will be processed in this environment O Both will be processed at the same time. Glucose O Not enough is known about the regulation of the lac operon to know which nutrient will be processed first Lactose Submit Request Answer
Like the lac operon, the hexose operon is controlled by a separate regulatory protein under the control of its own promoter (see the schematic of the operon below). The hexose regulatory protein is sensitive to fatty acyl CoA levels. When all hexose fuel sources are depleted, the bacteria switch to lipid metabolism and fatty acyl CoA levels increase. This turns expression of the hexose operon off. +1 +1 Regulatory Gene P regulator Hexose Operon Genes operon regulator promoter operon promoter e. The regulatory protein that controls expression of the hexose operons is a transcriptional ACTIVTOR or REPRESSOR (circle one).
Match each description with the appropriate label (allo)Lactose binds to the lac repressor, causing it to release from the promoter CAP binds CAMP to stimulate transcripti of the lac operon ✓ [Choose ] positive control of an inducible gene negative control of an inducible gene [Choose]
12a. In what condition is the LacZ gene transcribed? ANSWER (in one sentence) 12b. Fill in the following table about the Lac operon (LacZ gene) Environmental Condition + Glucose and + Lactose Glucose and + Lactose - Glucose and - Lactose + Glucose and - Lactose Q 2 Body" W #3 E D Which proteins bind to the LacZ promoter? ANSWER ANSWER ANSWER ANSWER $ 4 A R F B % 5 I T MacBook Pro A U 6 G Y & 7 H Is LacZ transcribed? (Yes or No) ANSWER ANSWER ANSWER ANSWER |||| U 8 K