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1. Describe the three different type of hemolysis that are observed on blood agar.
2. What is a selective medium?
3. What is a differential medium?
4. Which media can be used to isolate E. coli samples from contaminated lettuce?
5. Which two media would be used to identify a sample taken from a patient with suspected gonorrhea?
6. Would you be able to grow a sample obtained from a patient's wound (suspected to be infected with MRSA) on EMB? Explain.
7. What is the color or TSI for Salmonella?
8. What is a fastidious organism?
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- 1. Differentiate the tubed media. 2. Discuss the different streaking methods. 3. What is the streaking method used for Antimicrobial Susceptibility testing? 4. How will you determine if a culture is pure or mixed?1. Give 2 systems used at present for the automatic identification of bacteria. 2. Why aseptic technique important in urine collection for culture? 3. What are the specimen consideration you have to remember in preparing blood culture? 4. Being a medical technology student, how can you contribute to avoid water pollution and degradation of natural resources?A 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media. 3. What purpose differential diagnostic Endo media used for?
- 13. What is a differential agar? Why can it be of importance? 14. Describe how the following agars work: MacConkey, Blood, Phenylethyl alcohol, Mannitol salt. 15. What are the IMVIC series of tests? WHY / HOW are they used? 16. What is the catalase test? How is it used for colonies that are gram positive?NOTE: Kindly provide references. 1. Differentiate between defined and undefined culture media. 2. Define alpha, beta, and gamma-hemolytic reactions on blood agar media (BAM). Cite two (2) examples of bacteria causing the above hemolytic reactions.INSTRUCTIONS: Read each sentence carefully Encircle the biological techniques that are being described. 1. Itis also known as sterile technique. Aseptic technique Disinfection Answer: 2. It is described as the complete removal of microorganisms found in a specimen or sample. sterilization Disinfection Answer: 3. It is described as the elimination of pathogens that reduce the microbial contamination. sterilization Disinfection Answer: 4. This is described as the elimination of life processes in a specimen. Killing Fixation Answer: 5. Clearing is also known as Dehydration Dealcoholization Answer:
- 4. Which media can be used to isolate E. coli samples from contaminated lettuce? 5. Which two media would be used to identify a sample taken from a patient with suspected gonorrhea? 6. Would you be able to grow a sample obtained from a patient's wound (suspected to be infected with MRSA) on EMB? Explain. 7. What is the color or TSI for Salmonella? 8. What is a fastidious organism?1. What is one advantage of utilizing the pour plate technique over the streak plate technique ? 2. Why must the agar pours be cooled to 45C before use in the pour plate technique? 3. Explain the consequences if a group removed all the agar pours from the water bath at one time and allowed them to sit on the bench for several minutes before using them. 4. Why can the agar pour tubes be rinsed in the sink after the agar is transferred to the Petri plate ? Could you rinse the tubes if the bacteria had been pipetted into the agar pour tubes rather than in the plates? Explain. 5. What would be the result if a student dipped his / her loop in the stock culture during inoculations of each quadrant ? Explain . part B 1. The introduction stated that microbes are mechanically separated or diluted over the surface of the medium . How is this accomplished ? 2. Go to https://commons.wikimedia.org/wiki/File:MacConkey_agar_with_LF_and_LF_colonies . . Which side of the plate (left or right)…1. The differential aspect of MacConkey agar allows for the determination of which bacteria are lactose fermenters and which are not. 2. Blood agar is used to determine an organism's ability for hemolysis. Blood agar is differential in this regard. 3. EMB agar uses two dyes, eosin and methylene blue, to restrict the growth of gram-positive organisms, making this agar (EMB) in this regard. 4. Crystal violet dye in MacConkey agar will prevent some organisms from growing, making MacConkey agar in this regard. The crystal violet dye can also be used in the determination of the ability to ferment the carbohydrate in the agar; making MacConkey agar in this regard. 5. Use of an agar which contains an elevated amount of salt would allow some organisms to grow while preventing the growth of other organisms. This media would be considered selective
- What appropriate method should be used in controlling microbial growth in each of the following? 1. Chicken serum 2. Fresh goat milk 3. Nutrient broth 4. Paracetamol 5. Inoculation loob1.What advantage(s) does the pour plate method have over the streak-plate method? 2.Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the inoculation? 3.Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled to 50° C? 4.Explain why plates should be inverted during incubation. 5.Explain why plates should be inverted during incubation.1. What is the purpose of adding mineral oil to an O/F Media tube and why do we only put mineral oil in only one? 2. Which sugars are typically tested in the phenol red broth? Can the phenol red test be also used to determine if a certain bacterium can metabolize various carbohydrates? 3. Explain how conducting the MR/VP test too soon in a culture's growth cycle can lead to false negatives? 4. Generally, the color change we see in these tests is a result of what change in the media? Explain. 5. How does the production of urease acid aid Helicabactor. pylori ability to live in the stomach?