1. A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.90% NaCl. Three consecutive dilutions were further made by using 1 ml aliquot in 9 ml of 0.90% NaCl. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method.
Q: Kingdom: Animalia Phylum: Chordata Order: Dipnoi Suborder: Ceratodontoidei Family: Neoceratodontidae...
A: Neoceratodus forsterai is the only surviving lungfish species and is very old making it a living fos...
Q: what are some new, simple, and unique transgenic organism in today’s time that can be useful ?
A: Kingdom Animalia contains all animals. It is the largest kingdom of Animalia as compared to other ki...
Q: ee modes of dosage compensation exist in nature. riefly, describe the three different strategies. /h...
A: Males and females of several species have different sex chromosomes and in order to equalise their ...
Q: 4. Complete the table below Parent Cell(2N) Mitosis(2N) Meisios(n) 46 а. b. C. d.
A: Introduction Mitosis is an equational division in which the parent cell's chromosome number remains ...
Q: Explain characteristics of peranosporales ,agaricales, polyporales and ustilaginales in detail.
A: Answer
Q: Arrange the following sentences to create an explanation about the role of hormones in human reprodu...
A: 1.* Hypothalamus are of two types which release two types off harmones. Releasing harmones Inhibiti...
Q: Why is there a need for an incubator in the lab? How about incubating cultures at room temperature? ...
A: The Incubator is an insulated device in which the conditions like temperature, humidity, pH, and man...
Q: CHARACTERISTICS EUKARYOTIC CELLS PROKARYOTIC CELLS Has a nuclear envelope DNA structure 2+ chromosom...
A: Prokaryotic DNA is double-stranded circular DNA that is dispersed in the nucleoid, a dense area of c...
Q: The prairie is approximately 100 acres of relatively flat terrain. Considering the “basic” sampling ...
A: A... Forest Administration is presently carrying out an eating the executives plan at Sheyenne Publi...
Q: Today, it is easy to make transgenic plants and animals. What are some important safety and ethical...
A: Recombinant DNA technology Insertion of desired foreign DNA in an organism.
Q: What are some unknown and can be an interesting examples of trasgenic organisms present whose genes ...
A: Transgenic organisms- transgenic organisms are the organisms whose genes are genetically modified by...
Q: Please match the tissue to the description. covers the ends of bones in a joint [ Choose ] [ Choose ...
A: Cartilage. This is a type of tissue that covers the surface of a bone at a joint. Cartilage helps re...
Q: 1. IDENTIFY THE LINING. 2. IDENTIFY THE ORGAN. 3. IDENTIFY THE SPECIAL STRUCTURE. 1. 2. 3.
A: Introduction:- This organ starts from cricoid cartilage and finishes near the heart, where it splits...
Q: Give at least four types of food processing and explain its processes and applications.
A: Food Processing modification or changes to a plant or animal product after harvesting. Types of Foo...
Q: A scientist studying a species of algae, Gracilaria domingensis, discovered that its color is contro...
A: Alleles can be described as the different forms associated with particular gene. The formation of ge...
Q: - What is a pure culture? - Describe the procedure for making a streak plate from a clinical specime...
A: Introduction A growth media, also known as a culture medium, is a solid, liquid, or semi-solid mediu...
Q: When looking at TV with your legs stretched out, your messenteric arteries _________ so that blood c...
A: 41)Stretching of the legs causes increased blood supply of the arteries and decreased stiffness of t...
Q: DUE IN 30 MINUTES. This is multiple choice. No need for explanation, answer only. Thank you! A stra...
A: Basic amino groups (-NH2) and carboxyl groups (-COOH) are found in amino acids. Proteins have amino ...
Q: [ Select ] were needed in land plants to allow for gas exchange because the [ Select] created an imp...
A: Given: Like animals, plants do also respire. During respiration plants take in oxygen and given out ...
Q: What are 5 facts about fermentation?
A: Introduction: Pyruvic acid is a 3 carbon compound formed as the end product of glycolysis. Glycolys...
Q: Describe cyclic and non cyclic photo phosphorylation.
A: Photosynthesis is a metabolic process in which carbon dioxide react with water , sunlight and Chloro...
Q: Identify the pointed structures.
A: Connective Tissue is those tissues that support and protect the tissues and organs of the body. Some...
Q: ou have a farm with calves having issues with scours at 7-10 days of age. You want to esign a vaccin...
A: A.. An intestinal scour vaccine and injectable scour vaccine. B.. Calves are most at risk of calf sc...
Q: All of the following were unique challenges that life on land presented to land plants, E O Lack of ...
A: Plants are adapted to life on land by development of many structures like water repellant cuticle , ...
Q: Micronutrients play a critical role in assisting metabolic enzyme functions in our body? A. True B. ...
A: Enzymes are biocatalysts that are used to increase the rate of reaction and enzymes are not used up ...
Q: Show evident characteristics of a person growing to sexual
A: A person who is going through sexual maturity faces lots of change in the form of puberty. Puberty i...
Q: 7. Draw what you would expect to see for acid fast stain and endospore stain procedure if you did no...
A: Staining is a technique for adding color to cells, tissues, or microscopic components so that they c...
Q: Remove the agar plate from its lid with the left hand. While holding the plate, inoculate the agar h...
A: Introduction -- Microbes are around and every where in environment. Sterile agar plates which provid...
Q: II. Answer the following: 1. What does NADPH do? 2. In the Krebs cycle, isocitrate releases a molecu...
A: Introduction :- The tricarboxylic acid (TCA) cycle, commonly known as the Krebs or citric acid cycle...
Q: For laboratory accreditation, describe and briefly explain the assessment activities during the on‐s...
A: Accreditation of health laboratories is the process by which an independent and authorized agency ac...
Q: Fill in the blank with the most appropriate term that is described by the following statement: Close...
A: The components of DNA replication. A five-membered, oxygen-containing ribose sugar ring with three p...
Q: D. melanogaster
A: melanogaster, has been heavily used in research in genetics and is a common model organism in develo...
Q: B Which of the bacterial colonies shown (A or B) was nonlethal when injected in mice? A В
A: Introduction A bacterial colony is a collection of bacteria that all came from the same mother cell....
Q: When a fire is burning, a small fire leads to more fire. This is an example of: ________. Group of ...
A: Fire is an example of a chemical reaction. Forest fires are extremely dangerous. They have the abili...
Q: Which plant showed growth ? Why
A: Introduction Plant Hormones are also known as Phytohormones. Phytohormones are the signaling molecu...
Q: EPITHELIUM IN NO
A: Epithelium is refers to layers of cells that line hollow organs and glands.
Q: Explain how Bacara is used to isolate, differentiate and enumerate Bacillus cereus from food sample
A: Bacillus cereus is a spore-producing Gram-positive bacterium present in the soil. The spores produce...
Q: _________4. It is a bacterial that contains plasmid use to produce desirable proteins for human con...
A:
Q: The activated sludge process was developed from empirical knowledge and is now widely used for wast...
A: Activated sludge-The activated sludge process is a type of wastewater treatment process for treating...
Q: Differentiate between: a. Biotic and abiotic component
A: An ecosystem is shaped by its biotic component. These are communities of organisms made up of variou...
Q: 8. Label the phylogenetic tree and answer the questions. a. Place A on the tree at the location of t...
A: The phylogenetic tree is also called the evolutionary tree and it helps to determine the relationshi...
Q: 5.1) Do you expect DNA strands 1 and 2 below to have the same melting point? Justify your answer. S...
A: As per bartleby guidelines we are only allowed to answer one question. Please post the other questio...
Q: Ctenophora Animals Porifera Placozoa Cnidaria Bilateria |Chanoflagellata What is true about the red ...
A: The correct answer is- d) it includes the most recent ancestor and all of its descendants
Q: 4. The symptoms of many viruses come and go within 24 h. Use viral replication information t explain...
A: Viruses: A virus is a made up of protein and nucleic acid. It is submicroscopic infectious agent tha...
Q: Each time a new tRNA is added to the A site, it adds its amino acid to the bottom of the growing cha...
A: The description in the question is related to Protein synthesis or translation process which occurs...
Q: With what region of the promoter does the the principal interaction between it and the RNA polymeras...
A: Note: As Per Bartleby Guidelines For Remaining Answers Please Repost The Question. Introduction: The...
Q: Energy is stored long-term in the bonds of used short-term to perform work from a(n) molecule. and a...
A: Adenosine triphosphate (ATP) is the energy currency of all living cells. Cells need ATP molecules to...
Q: sequences oT two con the following bacterlal promoter. The startpoint of transcription is shown in r...
A: Transcription is the act of copying and interpreting information from a DNA strand into a new molecu...
Q: Match the concept in column A to the concept in column B. Write the letter of the correct answer on ...
A: Introduction "A cell is the smallest, most fundamental unit of life, responsible for all of life's o...
Q: Review of the Anatomy and Physiology of the brain Fil out the table below Section of the brain (fore...
A: Human brain The brain is the central information processing organ of our body and acts as as the 'c...
i. Illustrate the dilution series used and label the final dilution of each dilution.
Step by step
Solved in 3 steps with 1 images
- 1. A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population ofthe culture, by transferring 25 mlof the suspension to 225 ml 0.90% NaCl. Three consecutive dilutions were further made by using 1 mlaliquot in 9 mlof 0.90% NaCl. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. EDI Sampling COUNTS 1st dilution 54; 61 TNTC 2nd dilution 3; 7 TNCT TNTC 3rd dilution 0; 0 242; 233 TNTC 4th dilution 0, 0 28: 37 249;246 1st 2nd 3rd TNTC * TNTC = Too numerous to count i. Determine the bacterial count (CFU/ml) every 4 hours of incubation for 12 hours. Show all computations. a. 1s* Sampling b. 2d Sampling c. 3* SamplingA bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.90% NaCl. Three consecutive dilutions were further made by using I ml aliquot in 9 ml of 0.90% NaCl. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. 田 Sampling COUNTS 1st dilution 54; 61 2nd dilution 3rd dilution 4h dilution 1st 3; 7 0; 0 0,0 2nd TNTC TNCT 242: 233 28: 37 3rd TNTC TNTC INTC 249-246 * TNTC = Too numerous to count i. Illustrate the dilution series used and label the final dilution of each dilution. ii. Determine the bacterial count (CFU/ml) every 4 hours of incubation for 12 hours. Show all computations.A pure bacterial culture of unknown concentration was diluted to determine the concentration of viable bacteria in the original culture. Serial dilutions were performed as 2. diagrammed below. A volume of 500 µl was transferred into each tube. TSA plates were inoculated with 100 µl from the last three dilution tubes. a. If the dilution between each tube is 102, what is the volume of diluent in each of the 5 dilution tubes? Provide the volume using ml as the units. b. What is the total dilution of tube number 4? Express the total dilution using scientific notation. c. What is the concentration of viable bacteria in the original culture? Express the concentration using scientific notation and CFU/ml as the units. d. If you inoculated a TSA plate with 1.0 ml from dilution tube 4, how many colonies would you expect to form on the plate after incubation? e. If the original culture had a volume of 50ml, what was the total number of viable bacteria in the 50 ml of the original culture? 1 2 3…
- Assuming that serial dilution was carried out in a laboratory experiment in 6 tubes with 9 ml of saline in each tube and 0.1ml of the stock solution was used for initial dilution, pour plate method of inoculation was performed after the dilution process using 1ml of the culture from the samples from the test tubes. It was found out that on the 6th Petri dish after incubation, there was a total of 16 individual colonies counted. Compute the total number of microorganisms present in tube one, assuming that there was no human error in the transferring proces.assuming that serial dilution was carried out in a laboratory experiment in 6 tubes with 9 ml of saline in each tube and 0.1ml of the stock solution was used for initial dilution, pour plate method of inoculation was performed after the dilution process using 1ml of the culture from the samples from the test tubes. It was found out that on the 6th Petri dish after incubation, there was a total of 16 individual colonies counted. Compute the total number of microorganisms present in tube one, assuming that there was no human error in the transferring process.Five ml of bacterial culture is added to 45 ml of sterile diluent. From this suspension, the following serial dilutions were made, two 1:100 and one 1:10 dilutions, and 0.1 ml is plated onto Plate Count Agar from the last dilution. After incubation, 186 colonies were counted on the plate. 1. What is the dilution factor, or how much of the original sample was diluted?
- A pure bacterial culture of unknown concentration was diluted to determine the concentration of viable bacteria in the original culture. Serial dilutions were performed as 2. diagrammed below. A volume of 500 µl was transferred into each tube. TSA plates were inoculated with 100 µl from the last three dilution tubes. d. If you inoculated a TSA plate with 1.0 ml from dilution tube 4, how many colonies would you expect to form on the plate after incubation? e. If the original culture had a volume of 50ml, what was the total number of viable bacteria in the 50 ml of the original culture? 2 3 240 3 Number of coloniesYou are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL. List a series of dilutions and platings that you could carry out in order to determine the exact concentration of the culture. Note that you must plate four plates from a minimum of two dilution tubes. The volumes plated should be in the range of 0.1 mL – 1.0 mL. Duplicate volumes may not be plated from any one dilution tube. Each plating should aim for a count between 30 and 300 CFUs. You can select any value from 30-300 for CFU and any volume from 0.1-1.0 to find out dilution schemeTen grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of amL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further dilutedby successive 1/100 and 1/10 dilutions. One-tenth of a mL of this final solution was plated onto Plate Count agar.After incubation 145 colonies were present. How many colony-forming units were present in the total 10 gramsample of hamburger?
- A pure bacterial culture of unknown concentration was diluted to determine the concentration of viable bacteria in the original culture. Serial dilutions were performed as 1. diagrammed below. Each dilution tube contained 400 ul of diluent and 100 ul was transferred into each tube. TSA plates were inoculated with 100 µul from the last three dilution tubes. a. What is the dilution between each tube shown in the diagram below? Express your answer as a ratio. b. What is the total dilution of tube number 5? Express your answer as a ratio. c. What is the concentration of viable bacteria in the original culture? Express your answer using scientific notation and the units CFU/ml. d. What is the concentration of viable bacteria in tube number 2? Express your answer using the units CFU/ml. e. If you inoculated a TSA plate with 250 µl from tube number 5, how many colonies would you expect to see after the plate was incubated? 1 2 3 5 423 80 13 Number of coloniesFive mL of bacterial culture is added to 45 mL of sterile diluent. From this suspension, the following serial dilutions were made, two 1:100 and one 1:10 dilutions, and 0.1 mL is plated onto Plate Count Agar from the last dilution. After incubation, 186 colonies were counted on the plate1. Sally Monella found 344 bacterial colonies on one of her plates. She had prepared this plate after a serial dilution of a culture of Yersinia pestis. She had pipetted 1 ml sample of diluted Yersinia pestis from a tube with the overall dilution factor of 106 into a plate and covered it with agar. She used this plate to calculate the concentration in cells/ml of a bacterial culture. a) How many organisms were in the original culture? b) Were her results valid? Why or why not?