High performance team

Discussion In this experiment for part A, paper chromatography was used to separate and identify iron, nickel, and copper ions. In chromatographic separation, the filter paper was used as the stationary phase; the mobile phase was a mixture of HCl and acetone. The metal ions were carried along the stationary phase at different rates. This is helpful when an unknown mixture needs to be separated and identified. The components with lower molar masses or the less polar ones are weakly adsorbed on the

Crude Extract In order to produce crude extract, bovine tissue was obtained, precisely minced to exclude extra fat, and then blended with pH 7.2 phosphate buffer. The purpose of blending the tissue with the buffer was to pulverize the cells, causing them to release their contents evenly—most importantly lactate dehydrogenase (LDH)—into the solution. Since other cell components such as proteases which reduce LDH were also released from the lysed cells, the slurry was kept on ice to minimize their

The objective of the current study was to develop and validate a simple, accurate, precise and selective stability-indicating gradient reverse phase high performance liquid chromatographic method for simultaneous estimation of Ofloxacin (OFL) and Cefixime (CEF) in pharmaceutical formulation in presence of degradation products. The chromatographic separation of Ofloxacin and Cefixime was achieved on Shimadzu LC-20AT series HPLC having C18-ODS bonded column (250 ×4.6 mm, 40 °C, 10 μL) using UV/Visible

Pharmacopoeial and non-Pharmacopoeial specifications. Dissolution testing was undertaken using USP Apparatus 2 (Paddle Type), which allowed for a more realistic assessment and prediction of in vitro drug release rates. Samples were analysed using a high performance liquid chromatographic method (HPLC). Formulation F5 shows optimum drug release. Drug and rate retarding polymers ratio used in this formulation were Methocel K100 LV (14.86% w/w) and Methocel K4M (10.14%w/w), in ratio (5.4:1.34:1). The results

and benzoic acid are common additives to beverages for the stimulation effect and as a preservative, respectively. To simultaneously determine the amount of each of these chemicals, a method combining UV/Visible spectroscopy and reversed-phase high performance liquid chromatography was introduced. The experimentally determined concentrations at the 95% confidence interval of caffeine and benzoic acid in Mountain Dew were 149 +/- 5 ppm and 308 +/- 6 ppm, respectively. The method showed separation of

Metabolomics: A field of life science research that uses high throughput (HT) technologies to identify and or characterized all the small molecules or metabolites in a given cell, tissue or organ (I.e. the metabolite). Metabolite: Any natural atom discernible in the body with the sub-atomic weight (MW) under 1500 Dalton's. It incorporates liveliness tides, oligonucleotides, sugars, nucleosides, natural acids, ketones, aldehydes, amines, amino acids, steroids, alkaloids, nourishment added substances

yields high performance and high speed compared with traditional column chromatography because of the forcibly pumped mobile phase. Recently, ultrafast analysis using a high-pressure-resistant apparatus has been attracting attention. UHPLC (Ultra High Performance LC) is becoming established as an abbreviation for this ultrafast LC method. In 1941 Martin and Synge, described the discovery of liquid-liquid partition chromatography and also laid the foundation of Gas liquid chromatography and High performance

Lab Report 4: Protein Purification: Size Exclusion Chromatography Badrun Nessa Rahman Lab Partner: Briana Tolbert Section 55 Objective: The purpose of today’s laboratory is to understand how size exclusion chromatography plays a role in purifying protein which is an important concept to understand as it helps answer the mysteries which lay behind proteins such as

(1975). HPLC uses either a solid or liquid-coated solid stationary phase and a liquid mobile phase. Separation of analytes is achieved by adsorption of the analyte to the stationary phase. HPLC provides speed, resolution, high sensitivity and specificity (Lima, 2002). HPLC also be a lower temperature than GC methods; reducing the risk of isomerization of unsaturated fatty acids. HPLC has an immediate advantage over TLC, because reduces the exposure of the sample to atmospheric

Sophie Tran Chem 15 Prof. Neumark Nov 30. 2016 Partner: Megumi Mori GSI: Erin Experiment #8: Quantitative Analysis of a Solution Containing Cobalt and Copper I. Abstract: II. Introduction: This experiment will help students to get familiar with Ion Exchange Chromatography (IEC) which is used mostly to separate unknown mixture of metal ions. Anion and cation are known as two types of IEC. This column chromatography contains two phase such as stationary phase and mobile phase. In this experiment