Analytical Essay

The following results helped obtain the haplogroup that in which the sequence of mtDNA would identify. The PCR reaction worked, and this can be determined by looking at the agarose gel in figure 1. If the PCR reaction was successful, than a band should appear around 550bp. Individual AC displays a band around 550bp, this means the PCR reaction was successful. The band for individual AC, depicts a low concentration of product, because the band faint. After the purification process the concentration

The purpose of this experiment was to determine a series of external standards using Atomic Absorption (AA) and Flame Atomic Emission (FEA) spectroscopy. The principles of Atomic Absorption spectroscopy relies on the ability to distinguish one atom from another with great sensitivity as explained in Quantitative Chemical Analysis by Daniel Harris1. Specifically, this high precise determination can be achieved when specific wavelength of light is generated by a hollow-cathode lamp. The light emitted

High Performance Liquid Chromatography, abbreviated HPLC, is a type of Chromatography that uses liquids to separate mixtures. Unlike other chromatography methods, HPLC uses liquid mixtures for both the mobile and the stationary phases. A syringe is used to inject a small amount of the mixture we’re looking to separate into the HPLC column, and the mobile phase acts as the transporter. The mobile phase contains a carrier liquid that is comprised of all of the dissolved substances that are of interest

Chromatography is the term used to describe all the separation methods based on the distribution of compounds between two separate phases. TLC, an abbreviation for thin layer chromatography, is a chromatographic technique which is used to separate components of a mixture using a thin stationary which has an inert backing, this occurs as one phase is fixed on a plate (stationary phase) and the other phase is mobile and migrates through the stationary phase (mobile phase). During the chromatographic

Last semester’s Honors English lecture was on Sexual Identities in African American Literature. However, the topics often broadened to other areas, one of the most recurring being that of female sexuality and whether literature depicted it as pure and nearly asexual or tainted and virulent. I think this topic is a pressing issue in today’s culture; many states are fine-tuning abortion, rape, and gender-identification laws as I write this. I also saw much attention being paid to this topic in two

Chromatography is a technique used to separate mixtures of substances into their components^2. It can be used to various activities such as separating different pigment of dyes and inks all the way to analyzing DNA sequences. Chromatography works on the basis that different molecules have different polarities. By allowing molecules to travel through a polar surface, it is not surprising that molecules will different polarities will travel a different amount. The substance, or stationary phase

In this experiment, thin layer chromatography (TLC) was used to identify and compare polarity of two molecules, caffeine and acetaminophen. Chromatography is defined as the separation of a mixture of chemicals as they flow at different rates over a stationary phase based on their relative polarity. Caffeine, the more polar molecule had a greater affinity for the polar silica gel stationary phase causing it to consistently have a lower retention factor regardless of the mobile phase. This methodology

This experiment demonstrated the separation of pigments based on relative polarity and proved to be a substantial way to separate compounds. The results were much like that of an experiment performed, which separated carbohydrates in a very similar method with the use of paper chromatography (Inome, Y., & Yamamoto, A.). Proper pipetting technique, which is described by John Husler, was also demonstrated in this experiment. The technique was followed as to prevent contamination and deliver the right

Gas-liquid chromatography (GLC) is a process where an unknown organic sample is dissolved in a solvent then vaporised to separate it into its’ components. This is carried out by using two phases; the stationary phase and the mobile phase. The mobile phase is the gas containing the sample and the stationary phase is a liquid absorbed in a solid support. The liquid can be changed depending on the mixture being tested, so the stationary phase is packed in to a long, thin tube called the column (4college

Sanger settled instead on partition chromatography that had been developed in the1940s by Richard Synge and Archer Martin. This technique is that packed a tube tipped with ground up silica gel, then wetting the gel with water and pipetting in an amino acid solution at the top. Chloroform then inserted to wash the amino acid solution through and added a methyl red dye. The dye formed red bands against to an orange background which helped show up the separated amino acids. First tine Sanger tested