Analytical Essay

The first method to talk about is AAS. The general idea of AAS is to make up standard solutions of differing amounts of known concentrations of each of the metals to generate a calibration curve and then measure the absorption or emission of the unknown solution and then compare that value against the concentration curve to figure out the concentration of each metal in the sample. Atoms are put into the gas phrase for analysis. The process of getting the metal into the gas phase involves adding energy

Spectrophotometry is the process by which the light energy absorbed by colored compounds, is determined and used for quantitative and qualitative analyses. We use this process to often produce a standard curve, and use that to compare absorbance with different factors. In Experiment 3.1-3.4, spectrophotometry is used to produce the absorbance spectrums for the basic and acidic forms of PNP, to determine the pKa of PNP, and to develop standard curves for determination of unknown concentration and

The speciation of WO3 species and the effect of B2O3 on the coordination states of WO3 species were surveyed by the UV–vis DRS technique, and the results are shown in Fig 6. All the spectra were obtained by deducting the spectrum of the support UiO-66. For comparison, the UV–vis DRS spectrum of bulk WO3 is also presented, exhibiting a strong absorption band at 450 nm with a weak shoulder at 360 nm, accompanied by relatively weaker bands at 230 and 280 nm (Fig. 6A-a), and these bands are attributed

Vertigo is a psychological thriller film directed by Alfred Hitchcock, with a key theme of voyeuristic fetish and erotic obsession. The male protagonist, Scottie is depicted as the dominator in a heterosexual relationship with two female characters: Judy, who fakes Madeleine to seduce Scottie and deceive him to make false testimony for a planned murder, and Midge, the confidante of Scottie who is secretly in love with him. Through the interactions with Scottie, Judy and Midge are portrayed as submissive

Summarized Results To construct a calibration curve, five standard caffeine solutions are prepared and their retention times and peak areas are obtained from the High Performance Liquid Chromatography (HPLC) (Table 1). A graph of peak area versus concentration of caffeine standard solution is plotted (Figure 1). The slope of best fit line and intercept of the calibration curve are 97160384 and -3134906.6 (equation 1 and 2). The standard deviation of slope is 45811392.43 (equation 3). The standard

Methods Preparing Spin Labels R5 spin labels were placed within a “kink-turn motif” site where the internal loop between nucleotides that are not base paired (Figure 2). This site was held constant throughout the study in both the 20-nucleotide RNA study and the 232-nucleotide mRNA study. Both the synthetic 20-nucleotide long mRNA and the R5 spin label precursor were purchased from lab companies; the mRNA was purchased from Dharmacon, and the R5 spin labels were purchased from Toronto Research Chemicals

1. Two pieces of chromatography paper are trimmed so they are exactly 20.5 cm by 11 cm. The paper is handled by its edges only to avoid contamination from finger oils. 2. The mobile phase is prepared by dissolving a pinch of salt in 50 mL of room temperature distilled water. The salt is stirred until completely dissolved and set aside to use in step 12. 3. Enough mobile phase is transferred to the 400-600 mL beaker so that the liquid level is no more than 5 mm above the bottom of the beaker. The

When light is seen through the human eye, all the colors that are seen are those that are not absorbed by the substance. Spectrophotometry is the method used to measure how much a substance absorbs light by determining the intensity of the light. In 1852, The Beer-Lambert law of A= εlc (where A is the absorptivity, ε is the molar absorptivity, l is the path length of the cell, and c is the concentration) was created. The law shows that the absorptivity of a chemical substance is directly proportional

Thin-Layer Chromotography Discussion and Conclusion Easton Montgomery Discussion: In thin-layer chromatography a liquid is pumped across a bed of particles. The liquid that is pumped across is called the mobile phase and the particles are the stationary phase. A mixture of the molecules that will be separated is put into the mobile phase. Thin-layer chromatography tells you/helps you determine the number of compounds in a mixture, the purity of a compound, and the identity of compounds if you have

Instrument Operating Instruction 1. Ensure that the instrument Mate 100 is turned off. 2. Connect the power cord and then turn on the Mate 100. 3. Once the Mate 100 is turned on, the Main Screen will display. 4. On the Main Screen, select the Setting Screen. 5. On the Setting Screen, you can make the wanted concentration by increasing or decreasing of ▲▼ button. 6. Verify the density and viscosity of the original pure oil, and then make a set of supplying time of the pure oil. 7. At this time, you