Biological Science, Books a la Carte Plus Mastering Biology with Pearson eText -- Access Card Package (6th Edition)
6th Edition
ISBN: 9780134296029
Author: Scott Freeman, Kim Quillin, Lizabeth Allison, Michael Black, Greg Podgorski, Emily Taylor, Jeff Carmichael
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 9, Problem 14PIAT
PROCESS OF SCIENCE How could you determine if the mitochondrial ETC is affected in DNP-treated mice? Propose an experiment to determine if there is a correlation between life span and ETC activity in mitochondria isolated from mice used in the experiment above.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
In our feeding experiment with vermilion/brown and cinnabar/brown mutant flies, we were supposed to use kynurenine or hydroxykynurenine to overcome the enzymatic block in the ommochrome pathway, caused by the vermilion or cinnabar mutations. Which eye color would you expect for each of the two double mutants, if we had supplemented the medium with N-formylkynurenine? Use a diagram of the pathway to explain your answer.
Some living organisms are under considerable pressure tostreamline their genomes for the sake of more efficientoperation. As a result, the mitochondria of eukaryotic species have lost, to one degree or another, the overwhelmingmajority of their genes. During this process, several hundred mitochondrial genes were transferred to the nucleargenome. Yet mitochondria still retain a genome with thecapacity to produce several electron transport proteins.Review mitochondrial electron transport and suggest areason why these energy-generating organelles retained thegenes to produce this set of molecules.
You got an opportunity to join a professor lab who is working in-vivo model and specifically looking at the dysregulation of mitochondria in liver. He asked you to isolate mitochondria from a Rat liver and placed in an assay medium. Based on the knowledge you gain in this course so far, please answer the following questions:
a) Which technique will you use to isolate mitochondria?
b) What happens to the pH of the medium when the medium is kept anaerobic?
c) What happens when O2-saturated saline is added to the mixture?
Chapter 9 Solutions
Biological Science, Books a la Carte Plus Mastering Biology with Pearson eText -- Access Card Package (6th Edition)
Ch. 9 - Where does the citric acid cycle occur in...Ch. 9 - 2. What does the chemiosmotic hypothesis claim?
a....Ch. 9 - 3. After glucose is fully oxidized by glycolysis,...Ch. 9 - 4. What is the primary function of the reactions...Ch. 9 - Compare and contrast substrate-level...Ch. 9 - If you were to expose cells that are undergoing...Ch. 9 - In step 3 of the citric acid cycle, the enzyme...Ch. 9 - 8. Explain the relationship between electron...Ch. 9 - 9. Cyanide (C=N–) blocks complex IV of the...Ch. 9 - QUANTITATIVE Early estimates suggested that the...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Marilyn Houck and Margaret Kidwell proposed that P elements were carried from Drosophila willistoni to Drosophila melanogaster by mites that feed on fruit flies (M. A. Houck et al. 1991. Science 253:1125–1129). What evidence do you think would be required to demonstrate that D. melanogaster acquired P elements in this way? Propose a series of experiments to provide such evidence.arrow_forwardIf you could microinject cytochrome c into the cytosol of wild-type mammalian cell and another set that were mutated to be deficient in Bax and Bakl, what would you expect to happen to the wild-type (normal) cells? What about the mutated cells?arrow_forward1) Which of the following contains all of the information required to target a precursor protein from the cytosol to the mitochondrial matrix? a. N-terminal uptake-targeting sequence b. C-terminal uptake-targeting sequence c. post-translational organelle d. translocon e. None of the above 2) In the absence of targeting information, what is the default location of proteins synthesized on cytosolic ribosomes? a. Golgi apparatus b. ER lumun c. Cytosol d. Cell plasma membrane e. ER membranearrow_forward
- Yeast cells are eukaryotes, and they require a functional secretory pathway to grow and to maintain cellular organization. But surprisingly, when SRP is removed from yeast cells by deleting the relevant genes, the cells are still alive (although they grow slowly). a) How can yeast survive without SRP? Propose two alternative hypotheses. b) How might electron microscopy of normal and SRP-deficient yeast cells help you to distinguish between these two hypotheses?arrow_forwardSuppose you want to figure out what genes are involved in the production of proline (Pro) in a certain bacteria. (1) How would you go about designing a mutant screen to do so? (2) Suppose your screen identified 5 mutants that barely grew without being given Pro in the medium. How would you go about determining the number of different genes these mutants represent?arrow_forwardThe Perrier1 mutation of Chlamydomonas results in cells unable to generate ATP by oxidative phosphorylation. Which of the following is a possible genetic basis for this mutation? A. Defect to a nuclear gene that codes for a mitochondrial protein. B. Defect to a nuclear gene that codes for a cytosolic protein. C. Defect to a mitochondrial gene that codes for nuclear protein. D. Defect to a mitochondrial gene that gets translated on cytosolic ribosomes.arrow_forward
- You have constructed a recombinant vector harbouring pyruvate kinase. Subsequently, youwish to introduce your recombinant vector into Arabidopsis plants, since the enzyme isessential for the production of oil in developing seeds of Arabidopsis thaliana and oil crops.Explain how could you produce a transgenic Arabidopsis plant harbouring your syntheticgene by Agrobaterium tumefaciens-mediated transformation?arrow_forwardTreatment of cells with the drug brefeldin A (BFA) has the effect of uncoating Golgi membranes, resulting in a cell in which the vast majority of Golgi proteins are found in the ER. What inferences can be made from this observation regarding roles of coat proteins other than promoting vesicle formation? Predict what type of mutation in ARF might have the same effect as treating cells with BFA.arrow_forwardBriefly explain how heme regulates the expression of globin genes in cells that synthesize hemoglobin.arrow_forward
- Concerning the Tools of Genetics Box Analysis ofCell-Cycle Mutants in Yeast:a. Describe how you would use replica plating ofmutagenized, haploid yeast cells to identifytemperature-sensitive (ts) mutations in essentialgenes needed for yeast growth and survival.b. Among the many ts mutations you found in part(a), how would you distinguish mutations in genesneeded for cell-cycle progression from those ingenes needed for other aspects of the life of yeasts?c. If you had a large collection of yeast cell-cyclemutants, how would you determine which of themutations are in the same gene and which are indifferent genes?arrow_forwardA mutant haploid strain of Saccharomyces cerevisiae(yeast) called cox2-1 was found that was unable togrow on media containing glycerol as the sole sourceof carbon and energy. (Glycerol is a nonfermentablesubstrate for yeast.) This strain could, however, growon the fermentable substrate glucose. Researchers discovered that cox2-1 cells lack a mitochondrial proteincalled cytochrome c oxidase.a. Explain why cox2-1 cells can grow on mediumcontaining glucose but not on glycerol medium.arrow_forwardA mutant haploid strain of Saccharomyces cerevisiae(yeast) called cox2-1 was found that was unable togrow on media containing glycerol as the sole sourceof carbon and energy. (Glycerol is a nonfermentablesubstrate for yeast.) This strain could, however, growon the fermentable substrate glucose. Researchers discovered that cox2-1 cells lack a mitochondrial proteincalled cytochrome c oxidase.a. Explain why cox2-1 cells can grow on mediumcontaining glucose but not on glycerol medium.b. When cox2-1 was crossed with a wild-type yeaststrain and the resultant diploid cells were allowedto grow mitotically, it was found that about half thediploid clones were able to grow on glycerol, whilethe other half could not. The diploid clones thatcould grow on glycerol were induced to sporulate,and they yielded tetrads with four spores that wereall able to grow on glycerol medium. In all of thesetetrads, two of the haploid progeny were of matingtype a and two of mating type α. The diploids thatcould not…arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Biology: The Dynamic Science (MindTap Course List)BiologyISBN:9781305389892Author:Peter J. Russell, Paul E. Hertz, Beverly McMillanPublisher:Cengage Learning
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Cengage Learning
Mitochondrial mutations; Author: Useful Genetics;https://www.youtube.com/watch?v=GvgXe-3RJeU;License: CC-BY