The necessity of conducting TLC in a closed chamber and saturation of interior with vapor of the solvent should be determined. Concept introduction: Thin layer chromatography works on the basic principle of separation of the constituents of the sample based on its interaction with the mobile and the stationary phase. The sample depending on the extent of its polarity gets separated on the stationary phase under the influence of the mobile phase.

Question

2. Consider the following example GC Chromatogram from a publication about aggression among Mediterranean Acrobat Ants (C. scutellaris). This particular sample was generated by rinsing hydrocarbons from ant exoskeletons. From the chromatogram, what is the minimum number of unique compunds present in the sample? What data led you to that conclusion? (Don't overthink it) 2 8000000 7000000 6000000 5000000 4000000 3000000 2000000 1000000 Counts (Abundance) 5 19 6 7 0 23.00 24.00 10 | 11 13 17 20 14 15 16 12 teel | 22 19 21 ли 25.00 26.00 27.00 Time "Example of a Gas chromatography-Mass spectrography (GS-MS) chromatogram of hydrocarbon molecules in C. scutellaris" by Filippo Frizzi, et.al, in PLoS One is licensed under CC BY 4.0

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Question

Chapter 8, Problem 8Q

Interpretation Introduction

Interpretation:

The necessity of conducting TLC in a closed chamber and saturation of interior with vapor of the solvent should be determined.

Concept introduction:

Thin layer chromatography works on the basic principle of separation of the constituents of the sample based on its interaction with the mobile and the stationary phase. The sample depending on the extent of its polarity gets separated on the stationary phase under the influence of the mobile phase.

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2. Consider the following example GC Chromatogram from a publication about aggression among Mediterranean Acrobat Ants (C. scutellaris). This particular sample was generated by rinsing hydrocarbons from ant exoskeletons. From the chromatogram, what is the minimum number of unique compunds present in the sample? What data led you to that conclusion? (Don't overthink it) 2 8000000 7000000 6000000 5000000 4000000 3000000 2000000 1000000 Counts (Abundance) 5 19 6 7 0 23.00 24.00 10 | 11 13 17 20 14 15 16 12 teel | 22 19 21 ли 25.00 26.00 27.00 Time "Example of a Gas chromatography-Mass spectrography (GS-MS) chromatogram of hydrocarbon molecules in C. scutellaris" by Filippo Frizzi, et.al, in PLoS One is licensed under CC BY 4.0

Explain why TLC cannot be used for some volatile low molecular weight substances such as toluene.

Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.