Microbiology with Diseases by Body System (5th Edition)
5th Edition
ISBN: 9780134477206
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 8, Problem 3SA
Summary Introduction
To answer:
Use synthetic nucleic acid.
Introduction:
The recombinant DNA technology involves isolation of genetic materials (DNA) from donor organisms (human, plant, or microorganism) and inserts them into recipient organisms after genetic manipulation. The main tools involved in the techniques are
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what are the uses of nucleic acids?
a-Three things about how nucleic acids are used in cells:
b-Two other things found interesting about the uses of nucleic acids:
c- One questionwe can ask:
There may be more than one correct answer
The ability of DNA to denature is important for which of the following processes?
a.) DNA synthesis
b.) nucleic acid hybridization experiments
c.) RNA synthesis
d.) formation of biomembranes
You are trying to determine the base content for a number of samples in the lab (mouse DNA, bacterial DNA, single-stranded DNA virus and double-stranded RNA virus). Unfortunately, you failed to use an alcohol-proof marker and during the ethanol precipitation of your samples the labels came off the tubes. You finish the experiment anyway and try to use the data to determine the samples. Can you determine the source of each sample based on your results? Briefly explain how you come to your designations.
Sample
% adenine
% cytosine
% guanine
% thymine
% uracil
A
18
32
32
18
0
B
26
18
32
24
0
C
31
19
19
0
31
D
10
40
40
10
0
Chapter 8 Solutions
Microbiology with Diseases by Body System (5th Edition)
Ch. 8 - Why arent the terms recombinant DNA technology...Ch. 8 - Prob. 2TMWCh. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Prob. 5TMWCh. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MCCh. 8 - Prob. 4MCCh. 8 - Prob. 5MC
Ch. 8 - Prob. 6MCCh. 8 - Prob. 7MCCh. 8 - Prob. 8MCCh. 8 - Prob. 9MCCh. 8 - Prob. 10MCCh. 8 - Modified True/False 1. ________ Restriction...Ch. 8 - Modified True/False 2. ________ Restriction...Ch. 8 - Prob. 3MTFCh. 8 - Prob. 4MTFCh. 8 - Prob. 5MTFCh. 8 - Label the reagents and steps of PCR on the figure...Ch. 8 - Describe three artificial methods of introducing...Ch. 8 - Prob. 2SACh. 8 - Prob. 3SACh. 8 - Prob. 4SACh. 8 - List three potential problems of recombinant DNA...Ch. 8 - Examine the restriction sites listed in Table 8.1...Ch. 8 - CRITICAL THINKING 2 A cancer-inducing virus,...Ch. 8 - A thermocycler uses DNA polymerase from...Ch. 8 - Prob. 4CTCh. 8 - Prob. 5CTCh. 8 - Prob. 6CTCh. 8 - Prob. 7CTCh. 8 - Prob. 8CTCh. 8 - Prob. 9CTCh. 8 - Using the following terms, fill in the following...
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- Arrangement of nucleotides in DNA can be seen using which of the following instruments? a) Electron microscope b) Light microscope c) X-Ray crystallography d) Ultracentrifugearrow_forwardWatch the demonstration video on nucleic acid quantification on the NanoDrop spectrophotometer: https://cutt.ly/bio150dnaquantification Note: the video is entitled RNA but the method is identical for DNA quantification. 1. At what ratio of A260/280 can we say that DNA is pure? What about RNA and protein?2. While spectrophotometric methods are effective at detecting DNA, a more sensitive but expensive technique called fluorometry is used in sensitive applications. What is the principle behind fluorometry and why is it better than spectrophotometry in detecting DNA?arrow_forwardBefore X-ray diffraction and the discovery of the structure of deoxyribonucleic acid (DNA) was identified, what historical knowledge and technology existed? Explain previous models, theories and laws and technologies of that time. (Long response)arrow_forward
- Discuss the benefits of being able to predict protein structure from the DNA sequence (proteomics).arrow_forwardReview the table below. Nucleic acid from 4 different samples were analyzed to determine the amounts of nitrogenous bases in each. The amount is shown as a percentage. A G T U C Sample 1 22% 28% 22% 28% Sample 2 30% 30% 20% 20% Sample 3 18% 32% 18% 32% Sample 4 29% 29% 21% 21% What observations can you make from these data? Suggest at least two different observations. What conclusions can be supported by these observations? • Suggest one experiment that you could conduct to test or further investigate one of your conclusions from part b.arrow_forwardWhich of the following conventions is used to specify the sequence of bases in DNA a) The sequence begins with the nucleotide that has a free 3' terminus b) The sequence begins with the nucleotide that has a free 5' terminus c) The sequence begins from the end closest to the first adenine d) The sequence begins from the end closest to the first thyminearrow_forward
- Match the researchers (1–6) with the discoveries listed. Discovered regularity in the ratios of different bases in DNA. Worked out the helical structure of DNA by building Identified DNA as the genetic material in bacteriophages. Determined that DNA is responsible for transformation in bacteria. Demonstrated that heat-killed material from bacteria can genetically transform live bacteria. Took X-ray diffraction pictures used in constructing the structure of DNA. 1. Watson and Crick 2. Hershey and Chase 3. Avery, MacLeod, and McCarty 4. Griffith 5. Franklin and Wilkins 6. Chargaffarrow_forwardFor what purpose is DNA fingerprinting used A) to sequence DNA from bacteria B) to separate DNA fragments C) to identify individuals who have committed crimes D) to identify single nucleotide polymerasearrow_forwardA DNA molecule consists of 500 nitrogenous bases, of which 150 are G bases. So, the percentage of nitrogenous base T is equal to? choose the correct answer A)20% B)30% C)40% D)60%arrow_forward
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