Concept explainers
To answer:
Use synthetic nucleic acid.
Introduction:
The recombinant DNA technology involves isolation of genetic materials (DNA) from donor organisms (human, plant, or microorganism) and inserts them into recipient organisms after genetic manipulation. The main tools involved in the techniques are
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Microbiology with Diseases by Body System (5th Edition)
- Watch the demonstration video on nucleic acid quantification on the NanoDrop spectrophotometer: https://cutt.ly/bio150dnaquantification Note: the video is entitled RNA but the method is identical for DNA quantification. 1. At what ratio of A260/280 can we say that DNA is pure? What about RNA and protein?2. While spectrophotometric methods are effective at detecting DNA, a more sensitive but expensive technique called fluorometry is used in sensitive applications. What is the principle behind fluorometry and why is it better than spectrophotometry in detecting DNA?arrow_forwardDescribe the “blender experiments” of Hershey and Chase and what they revealed about DNA versus protein?arrow_forwardDiscuss the benefits of being able to predict protein structure from the DNA sequence (proteomics).arrow_forward
- Review the table below. Nucleic acid from 4 different samples were analyzed to determine the amounts of nitrogenous bases in each. The amount is shown as a percentage. A G T U C Sample 1 22% 28% 22% 28% Sample 2 30% 30% 20% 20% Sample 3 18% 32% 18% 32% Sample 4 29% 29% 21% 21% What observations can you make from these data? Suggest at least two different observations. What conclusions can be supported by these observations? • Suggest one experiment that you could conduct to test or further investigate one of your conclusions from part b.arrow_forwardIf you were going to design a drug to fight a virus, what would be likely targets for the drug design?arrow_forwardMatch the researchers (1–6) with the discoveries listed. Discovered regularity in the ratios of different bases in DNA. Worked out the helical structure of DNA by building Identified DNA as the genetic material in bacteriophages. Determined that DNA is responsible for transformation in bacteria. Demonstrated that heat-killed material from bacteria can genetically transform live bacteria. Took X-ray diffraction pictures used in constructing the structure of DNA. 1. Watson and Crick 2. Hershey and Chase 3. Avery, MacLeod, and McCarty 4. Griffith 5. Franklin and Wilkins 6. Chargaffarrow_forward
- Explain how Avery-MacLeod-McCarty experiment discovered that DNA is the genetic material.arrow_forwardYou have isolated a new bacteriophage and determined that the composition of its is nucleic acid is 17% A, 26% C, 34% G, and 23% T. What would you conclude about its genome? (One sentence, please)arrow_forwardWhy do we study the DNA sequence of human organism?arrow_forward
- Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning