Polymerase chain reaction (PCR) is a rapid method involved in the amplification of a specific segment of the DNA molecule. This technique is used to generate a billion copies of the identical molecule of the DNA segment within a few hours (nearly 30 cycles). It is used for clinical studies and the evaluation of genomic variations. The important components used in PCR reactions are DNA, primer, deoxyribonucleotide triphosphates (A, T, G, and C) or dNTPs, Taq DNA polymerase, MgCl2, and buffer. Denaturation (separation of double-stranded DNA into two single-strands of DNA), annealing (binding of primers to ends of single-stranded DNA), and extension (addition of dNTPs at 3’ site of the primer by DNA polymerase) are the three main steps of PCR in each cycle. The amplified products are analyzed using electrophoresis technique.

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Chapter 8, Problem 3MTF

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Introduction:

Polymerase chain reaction (PCR) is a rapid method involved in the amplification of a specific segment of the DNA molecule. This technique is used to generate a billion copies of the identical molecule of the DNA segment within a few hours (nearly 30 cycles). It is used for clinical studies and the evaluation of genomic variations. The important components used in PCR reactions are DNA, primer, deoxyribonucleotide triphosphates (A, T, G, and C) or dNTPs, Taq DNA polymerase, MgCl2, and buffer. Denaturation (separation of double-stranded DNA into two single-strands of DNA), annealing (binding of primers to ends of single-stranded DNA), and extension (addition of dNTPs at 3’ site of the primer by DNA polymerase) are the three main steps of PCR in each cycle. The amplified products are analyzed using electrophoresis technique.

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CRITICAL THINKING 1. Nitrogen deficiency stunts plant growth and causes leaves to turn yellow and then die. Why does nitrogen deficiency cause these symptoms? Hint: Think about which biological molecules incorporate nitrogen atoms.

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Chapter 8, Problem 3MTF

Summary Introduction

Introduction:

Polymerase chain reaction (PCR) is a rapid method involved in the amplification of a specific segment of the DNA molecule. This technique is used to generate a billion copies of the identical molecule of the DNA segment within a few hours (nearly 30 cycles). It is used for clinical studies and the evaluation of genomic variations. The important components used in PCR reactions are DNA, primer, deoxyribonucleotide triphosphates (A, T, G, and C) or dNTPs, Taq DNA polymerase, MgCl2, and buffer. Denaturation (separation of double-stranded DNA into two single-strands of DNA), annealing (binding of primers to ends of single-stranded DNA), and extension (addition of dNTPs at 3’ site of the primer by DNA polymerase) are the three main steps of PCR in each cycle. The amplified products are analyzed using electrophoresis technique.

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Answer 3

Question

Chapter 8, Problem 3MTF

Summary Introduction

Introduction:

Polymerase chain reaction (PCR) is a rapid method involved in the amplification of a specific segment of the DNA molecule. This technique is used to generate a billion copies of the identical molecule of the DNA segment within a few hours (nearly 30 cycles). It is used for clinical studies and the evaluation of genomic variations. The important components used in PCR reactions are DNA, primer, deoxyribonucleotide triphosphates (A, T, G, and C) or dNTPs, Taq DNA polymerase, MgCl2, and buffer. Denaturation (separation of double-stranded DNA into two single-strands of DNA), annealing (binding of primers to ends of single-stranded DNA), and extension (addition of dNTPs at 3’ site of the primer by DNA polymerase) are the three main steps of PCR in each cycle. The amplified products are analyzed using electrophoresis technique.

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Answer 4

Question

Chapter 8, Problem 3MTF

Summary Introduction

Introduction:

Polymerase chain reaction (PCR) is a rapid method involved in the amplification of a specific segment of the DNA molecule. This technique is used to generate a billion copies of the identical molecule of the DNA segment within a few hours (nearly 30 cycles). It is used for clinical studies and the evaluation of genomic variations. The important components used in PCR reactions are DNA, primer, deoxyribonucleotide triphosphates (A, T, G, and C) or dNTPs, Taq DNA polymerase, MgCl2, and buffer. Denaturation (separation of double-stranded DNA into two single-strands of DNA), annealing (binding of primers to ends of single-stranded DNA), and extension (addition of dNTPs at 3’ site of the primer by DNA polymerase) are the three main steps of PCR in each cycle. The amplified products are analyzed using electrophoresis technique.

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Answer 5

Chapter 8, Problem 3MTF

Summary Introduction

Introduction:

Polymerase chain reaction (PCR) is a rapid method involved in the amplification of a specific segment of the DNA molecule. This technique is used to generate a billion copies of the identical molecule of the DNA segment within a few hours (nearly 30 cycles). It is used for clinical studies and the evaluation of genomic variations. The important components used in PCR reactions are DNA, primer, deoxyribonucleotide triphosphates (A, T, G, and C) or dNTPs, Taq DNA polymerase, MgCl2, and buffer. Denaturation (separation of double-stranded DNA into two single-strands of DNA), annealing (binding of primers to ends of single-stranded DNA), and extension (addition of dNTPs at 3’ site of the primer by DNA polymerase) are the three main steps of PCR in each cycle. The amplified products are analyzed using electrophoresis technique.

Answer 6

Chapter 8, Problem 3MTF

Summary Introduction

Introduction:

Polymerase chain reaction (PCR) is a rapid method involved in the amplification of a specific segment of the DNA molecule. This technique is used to generate a billion copies of the identical molecule of the DNA segment within a few hours (nearly 30 cycles). It is used for clinical studies and the evaluation of genomic variations. The important components used in PCR reactions are DNA, primer, deoxyribonucleotide triphosphates (A, T, G, and C) or dNTPs, Taq DNA polymerase, MgCl2, and buffer. Denaturation (separation of double-stranded DNA into two single-strands of DNA), annealing (binding of primers to ends of single-stranded DNA), and extension (addition of dNTPs at 3’ site of the primer by DNA polymerase) are the three main steps of PCR in each cycle. The amplified products are analyzed using electrophoresis technique.

Answer 7

Chapter 8, Problem 3MTF

Summary Introduction

Introduction:

Polymerase chain reaction (PCR) is a rapid method involved in the amplification of a specific segment of the DNA molecule. This technique is used to generate a billion copies of the identical molecule of the DNA segment within a few hours (nearly 30 cycles). It is used for clinical studies and the evaluation of genomic variations. The important components used in PCR reactions are DNA, primer, deoxyribonucleotide triphosphates (A, T, G, and C) or dNTPs, Taq DNA polymerase, MgCl2, and buffer. Denaturation (separation of double-stranded DNA into two single-strands of DNA), annealing (binding of primers to ends of single-stranded DNA), and extension (addition of dNTPs at 3’ site of the primer by DNA polymerase) are the three main steps of PCR in each cycle. The amplified products are analyzed using electrophoresis technique.

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Ch. 8 - Why arent the terms recombinant DNA technology...Ch. 8 - Prob. 2TMW Ch. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Prob. 5TMW Ch. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MC Ch. 8 - Prob. 4MC Ch. 8 - Prob. 5MC

Solution Summary: The author explains that polymerase chain reaction (PCR) is used to generate billion copies of the identical DNA segment within a few hours. The amplified products are analyzed using electrophoresis technique.

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