The type of mutation preferable for the reversion in Ames test: deletion, point mutation, or a frameshift mutation. Introduction: Mutations are the permanent alteration in the nucleotide sequence of the genome of organism. These are of three different types: point mutation, where a single nucleotide base is changed, deletion mutation, where a nucleotide base is deleted and frameshift mutation where reading frame of genome changes either by insertion or deletion of nucleotide.

Question

Want to see the full answer?

Answer 1

Question

Chapter 8, Problem 1CT

Summary Introduction

To analyze:

The type of mutation preferable for the reversion in Ames test: deletion, point mutation, or a frameshift mutation.

Introduction:

Mutations are the permanent alteration in the nucleotide sequence of the genome of organism. These are of three different types: point mutation, where a single nucleotide base is changed, deletion mutation, where a nucleotide base is deleted and frameshift mutation where reading frame of genome changes either by insertion or deletion of nucleotide.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

You have a patient in your clinic presenting symptoms of cystic fibrosis. You screen their CFTR gene for mutations, and find the following list: CFTR 320 L V CFTR 341 S W CFTR 528 E D CFTR 976 F Q CFTR 1235 S R Which mutation(s) are likely causing cystic fibrosis in this patient? You also sequence a newborn family member of this patient. They have all of these same mutations, other than the one at position 976, and no other mutations in CFTR. Do you predict this person will develop cystic fibrosis? Explain why.

Cystic fibrosis (CF) is a genetic disorder affecting a number of organs, including the lung airways, pancreas, and sweat glands. Mutations in both copies of the CFTR gene causes cystic fibrosis. Imagine that you have sweat gland samples from several Cystic Fibrosis patients (A-C) with unknown mutations in CFTR. You also have normal (+) sweat gland sample to use as a positive control. А В С А В С Choose which mutation would explain the RNA and protein results in A, B, & C: 1. Promoter/Regulatory mutation 2. Silent mutation 3. Missense mutation 4. Deletion mutation 5. Splice site mutation 6. Nonsense mutation RNA gel Protein gel

Leber Congenital Amaurosis (LCA) causes progressive vision loss due to defects in the gene that encodes RPE65 isomerase. Affected individuals are homozygous recessive for mutant alleles of the RPE65 gene. You are trying to determine the molecular nature of the mutations in three individuals with LCA. For ease of analysis, you may assume that each individual is homozygous for the same mutant allele (though the three individuals have different mutations than each other). You use the polymerase chain reaction to amplify DNA from each patient and you determine the sequence of the DNA and compare it to unaffected individuals. You identify the following differences. Note that the non-template strand of DNA is given and the changes are highlighted using red boldface. You can assume that the sequences are in the first reading frame (eg. the first three nucleotides of each sequence is a codon). The coding region of the gene is 1602 bp and the position of the sequences shown below is…