Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 7.4, Problem 3CC
Why would cells that are vigorously growing when inoculated into fresh culture medium have a shorter
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A culture with approximately 4x105 cells/mL were incubated. After 10 hours, the number of cells had increased to 5x109.
a) How long was the generation time in minutes?b) How many generations have occurred?
starting with four bacterial cells per milliliter in a rich nutrient medium, with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in 1 ml of this culture after 10 hours?
Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in a liter of medium at the end of 2 hours? At the end of 7 hours? Show your solution.
Chapter 7 Solutions
Prescott's Microbiology
Ch. 7.1 - MICRO INQUIRY In addition to chromosomes, what...Ch. 7.2 - MICRO INQUIRY Why is it important that the origin...Ch. 7.2 - MICRO INQUIRY What would be the outcome if FtsZ...Ch. 7.2 - MICRO INQUIRY Which step in the development of...Ch. 7.2 - Retrieve, Infer, Apply 1. Describe the three...Ch. 7.2 - Retrieve, Infer, Apply 2. How does the bacterial...Ch. 7.2 - Retrieve, Infer, Apply 3. Do you think MinCDE...Ch. 7.2 - Retrieve, Infer, Apply 4. Do you think Spiroplasma...Ch. 7.3 - What elements of the Sulfolobus spp. cell cycle...Ch. 7.3 - Many archaea have genes encoding an FtsZ...
Ch. 7.4 - MICRO INQUIRY Identify the regions of the growth...Ch. 7.4 - Retrieve, Infer, Apply Define microbial growth.Ch. 7.4 - Retrieve, Infer, Apply Describe the phases of the...Ch. 7.4 - Why would cells that are vigorously growing when...Ch. 7.4 - Calculate the growth rate constant and generation...Ch. 7.4 - Suppose the generation time of a bacterium is 90...Ch. 7.5 - What is the difference between halophilic and...Ch. 7.5 - Why do facultative anaerobes grow best at the...Ch. 7.5 - Retrieve, Infer, Apply 1. How do microorganisms...Ch. 7.5 - Retrieve, Infer, Apply 2. Define water activity...Ch. 7.5 - Prob. 1.3CCCh. 7.5 - Retrieve, Infer, Apply 1. Define pH, acidophile,...Ch. 7.5 - Retrieve, Infer, Apply Classify each of the...Ch. 7.5 - Retrieve, Infer, Apply 3. Describe the mechanisms...Ch. 7.5 - Retrieve, Infer, Apply 1. What are cardinal...Ch. 7.5 - Prob. 3.2CCCh. 7.5 - Prob. 3.3CCCh. 7.5 - Prob. 3.4CCCh. 7.5 - Retrieve, Infer, Apply Describe the five types of...Ch. 7.5 - Retrieve, Infer, Apply What are the toxic effects...Ch. 7.5 - Retrieve, Infer, Apply Where would you expect to...Ch. 7.5 - Retrieve, Infer, Apply List the types of...Ch. 7.5 - Prob. 5.3CCCh. 7.5 - Prob. 5.4CCCh. 7.6 - MICRO INQUIRY What biomolecules make up the...Ch. 7.6 - Prob. 1CCCh. 7.6 - Prob. 2CCCh. 7.6 - Prob. 3CCCh. 7.6 - Retrieve, Infer, Apply What is quorum sensing?...Ch. 7.6 - Retrieve, Infer, Apply How is the communication...Ch. 7.7 - Prob. 1CCCh. 7.7 - What are peptones, yeast extract, beef extract,...Ch. 7.7 - Retrieve, Infer, Apply Describe four ways in which...Ch. 7.7 - What are pure cultures and why are they important?...Ch. 7.7 - Retrieve, Infer, Apply It is known that microbial...Ch. 7.7 - Retrieve, Infer, Apply How might an enrichment...Ch. 7.8 - Why is it important to have no more than about 250...Ch. 7.8 - Briefly describe each technique by which microbial...Ch. 7.8 - Prob. 2CCCh. 7.8 - Prob. 3CCCh. 7.8 - For each of the following, which enumeration...Ch. 7.9 - Prob. 1MICh. 7.9 - Prob. 1CCCh. 7.9 - Prob. 2CCCh. 7.9 - Prob. 3CCCh. 7 - Prob. 1RCCh. 7 - Prob. 2RCCh. 7 - Prob. 3RCCh. 7 - Prob. 4RCCh. 7 - Prob. 5RCCh. 7 - Prob. 6RCCh. 7 - As an alternative to diffusible signals, suggest...Ch. 7 - If you wished to obtain a pure culture of bacteria...Ch. 7 - Prob. 3ALCh. 7 - Suggest one specific mechanism underlying the...Ch. 7 - Consider cell-cell communication: bacteria that...Ch. 7 - Suppose you discovered a new bacterial strain from...Ch. 7 - Prob. 7ALCh. 7 - Prob. 8ALCh. 7 - Prob. 9AL
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- Assume an inoculum with a cell density of 108 cells per mL. The entire generation time takes 30 minutes. How many hours would it take to grow a culture to 108/mL if you started with a 10–2 dilution? helpful formula: g (generation time) = 0.301 (time)/ log x – log xoarrow_forwardWhat is the advantage of the plating method over an electronic cell counting method in counting cells? Why does turbidity lose reliability at high cell concentrations when the culture reaches the stationary phase?arrow_forwardWhy after a certain period the growth rate of the culture decreases until growth stops?arrow_forward
- You have a starter culture containing 8 x 109 cells per mL, from which you take 10mL to inoculate a fresh 1 L culture. After 15 hours, the cell density of the new culture is 3 x 1012cells per mL. What are the generation time and the mean growth rate constant of theorganism in culture?arrow_forwardAssume you have a stock culture at 5 x 109 cells/mL and you wish to inoculate 1 liter of fresh medium so that in 15 hours the cell density will be 2 x 108/mL. Assume a generation time of 3.5 hours. What should be the dilution?arrow_forwardThere are two cultures of yeast cells in the pictures, one has been incubated for 6 hours and one has been incubated for 24 hours. After a 10x dilution by taking 100µl of each culture and adding it to 900 µl water in a microcentrifuge tube and 100µl sample from the tube was taken to view in the counting chamber. a) Count the total number of yeast cells for each culture respectively b) Calculate the concentration and density of yeast cells for each culture respectivelyarrow_forward
- If a culture had 4 cells to begin with and has a generation time of 60 minutes, how long would it take to get 1,048,576 cells?arrow_forwardA bacterial culture contains 500 cells/mL in the exponential growth phase at 8 AM. If you consider a generation time 20 minutes, what will be the total number of cells/mL after three hours (i.e. at 11 AM)?arrow_forwardYou just finished plating your electroporatio products on your YPD-Zeocin plate and you think you did everything perfectly but you come back the folloeing week and have zero colonies. Which of the following could be the reason for the absence of colonies? A) You centrifuged your electroporated cells for 30 sec at 16,000 rpm instead of 4,000 rpm before plating them B) You added sorbitol+YPD to your cells thirty minutes after pulsing your cells C) The Zeocin had not been added to the plates when they were made. D) All of the above E) Both a and b onlyarrow_forward
- How long does it take for a bacterial culture with a generation time of 12 minutes to increase from 400 cells up to 1600 cells/mL?arrow_forwardOn agar plate does each discrete colony represent the growth of one cell? Explain your answer. Why can a single colony on a plate be used to start a pure culture?arrow_forwardCalculate the growth rate and generation time of a culture that increases in the exponential phase from 5 x 102 to 1 x 108 cells in 12 hours.arrow_forward
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