WHAT IS LIFE? GDE.TO BIOLOGY W/PHYSIO.
5th Edition
ISBN: 9781319272531
Author: PHELAN
Publisher: MAC HIGHER
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Chapter 7, Problem 3MC
Summary Introduction
Introduction:
Gene therapy is a treatment method in which a healthy, functional gene is introduced into a person having a defective, disease-causing gene, so that the functional gene can compensate for the defective gene.
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WHAT IS LIFE? GDE.TO BIOLOGY W/PHYSIO.
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- Using retroviral vectors for gene therapy might increase the patient's risk of developing cancer because they might: a. introduce proteins from the virusb. integrate recombinant DNA into the genome in ways that misregulate the expression of genes at or near the site of integrationc. not express the genes that were introduced into a patient's cellsd. not integrate their recombinant DNA into the patient's genomearrow_forwardWhich of the following is a concern of in-vivo gene therapy? A. Lytic vector expression B. Integration into target region C. Immune response to viral vector D. Production of chimeraarrow_forwardWhy are vaccines and/or passive immunization the method of choice in the treatment or prevention of viral infections? A. There are no known inhibitors to reverse transcription B. Viruses are non-living and do not have their own genetic mechanisms to reproduce by themselves hence host cells must rely on targeting the virus or their products. C. Antibodies prevent replication of viral genome D. Antibodies prevent transcription and translation of viral genomes E. All of thesearrow_forward
- If the HIV did not have a reverse transcriptase enzyme, then which of the following would apply? Select one: a. The virus can make use of the reverse transcriptase enzyme from the host cell to convert its RNA into DNA b. Viral particles will be produced without reverse transcriptase enzyme since the original HIV is an RNA virus and needs to assemble RNA ant coat proteins together c. The virus will manufacture its proteins from its RNA using translation and ribosomes from host cell d. Provirus will not be formed and assembly of viral particles will never occur e. The virus will not be able to recognise the host cell and therefore entry into the cell is blockedarrow_forwardWhich of the following best describes the approach that wasused in the first gene therapy trial for treating SCID?a. The normal ADA gene was introduced by injecting liposomesdirectly into the patients’ bodies. b. Lymphocytes were removed from a SCID patient, the normalADA gene was transferred into the lymphocytes vialiposomes,and then the lymphocytes were returned to thepatient’s body.c. Lymphocytes were removed from a SCID patient, the normalADA gene was transferred into the lymphocytes via a retrovirus,and then the lymphocytes were returned to thepatient’s body.d. None of the above describe the approach used in the trial.arrow_forwardImmunofluorescence and GPF tagging are most commonly used to... a. localize proteins within a cell or organism. b. localize mRNA inside a cell or organism. c. deduce the function of specific genes within an organism. d. follow a signal transduction pathway within a cell or organism.arrow_forward
- Viruses like HIV can insert their genome into the host nucleus. This can result in: a. latent infection. ○ b. hyper-immune response. ○ c. mutagenesis due to DNA disruption.arrow_forwardYou would like to create an anti-viral medication that is active against both H5N2 and H5N1 strains of influenza. The best drug target would be: a. ribosomal protein production b. Hemagglutinin-mediated fusion c. Neuroaminidase-mediated buddingarrow_forwardWhich of the following statements is not true? Select one: a. Viruses multiply inside living cells using viral - mRNA, tRNA & ribosomes. b. Viruses multiply inside living cells. c. Viruses contain DNA or RNA. d. The nucleic acid of a virus is surrounded by a protein coat. e. Viruses cause the synthesis of specialized infectious elements.arrow_forward
- Which nucleic-acid based technique will detect norovirus? A. Fluorescent In Situ Hybridization (FISH) probes B. PCR with primers specific for the genome of this virus C. Terminal Restriction Fragment Length Polymorphism (T-RFLP) D. reverse transcription PCR with specific primers A patient's blood sample tests positive for the antigen to Vibrio cholerae. Which of the following interpretations is correct? A. This person has cholera. B. This person was exposed to any species of Vibrio. C. This person was exposed to V. cholerae recently. D. This person had cholera at some point in their lifetime.arrow_forwardSome strains of Escherichia coli bacteria have acquired the ability to produce the harmful Shiga toxin, normally produced by Shigella dysenteriae. Which statements best explain why this occurs? Mark all that apply. A. conjugation between two different species of bacteria allowed both strains of bacteria to express a virulence factor B. transformation resulted in expression of a virulence factor C. horizontal gene transfer between different species of bacteria D. transduction of a specific gene resulted in expression of new traitsarrow_forwardA particular strain of λ (lambda) can lysogenize its E. coli host at 30°C, but not at 42°C. Could a temperature-sensitive mutation in the int (integrase) gene explain this phenotype? A. There is insufficient information to answer the question. B. No C. Yesarrow_forward
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