WHAT IS LIFE? GDE.TO BIOLOGY W/PHYSIO.
5th Edition
ISBN: 9781319272531
Author: PHELAN
Publisher: MAC HIGHER
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Chapter 7, Problem 2MC
Summary Introduction
Introduction:
PCR is a technique used to amplify the DNA.
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Which of the following statements about DNA probes are false?
a. Probes can be labeled with a fluorescent dye to facilitate detection.
b. Probes can be labeled with radioactive phosphorus to facilitate detection.
c. Probes are typically composed of double stranded pieces of DNA.
d. Probes are typically composed of single stranded pieces of DNA.
Which of the following is true about DNA manipulation? Select the best answer.
A. all of these
B. DNA has never been transferred between different organisms.
C. Transferring DNA from one organism into another always kills the host organism.
D. Whole genomes have been manipulated by humans for centuries; now individual genes ca be inserted into different organisms using a variety of techniques.
E. DNA can't be manipulated.
Which of the following describes an advantage of using a recombinant plasmid for DNA cloning over PCR?
A. PCR is more likely to have errors introduced in the copying process.
B. Recombinant DNA plasmids are able to create large amounts of copies more quickly than PCR.
C. PCR can only be conducted in eukaryotic cells.
D. PCR requires prior knowledge of the sequence in question, while a recombinant plasmid does not.
Chapter 7 Solutions
WHAT IS LIFE? GDE.TO BIOLOGY W/PHYSIO.
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Transcriptomes, that is, all the transcripts in a cell, are analyzed using: a. a type of PCR b. fluorescently labeled nucleotides c. microarrays d. all the abovearrow_forwardWhich of the following is NOT true of DNA polymerase? a. DNA polymerase builds the new strand from 5' to 3'. b. DNA polymerase can start new DNA strands independently. c. DNA polymerase uses a "guess and check" method to add nucleotides. d. DNA polymerase uses dATP as a nucleotide. e. DNA polymerase reads the template from 3' to 5'.arrow_forwardWhen constructing a recombinant DNA molecule, a marker gene is used to: a. give the organism a new trait, such as insect resistance b. Identify whether the transformed organism contains the recombinant DNA c. replicate (copy) the gene of interest d. Introduce the recombinant DNA into an organism e. cut short sequences of DNAarrow_forward
- Definition of Terms: a. Genetic Engineering b. DNA c. Recombinant DNA d. Plasmids e. Cloning f. Genome g. Gene Mapping h. Biotechnology i. Polymerase Chain Reaction j. Gene Therapyarrow_forwardWhich statement is true? a. There is no danger involved in recombinant DNA research in humans. b. Stringent safety rules make the use of recombinant DNA research impossible. c. There is no danger in releasing recombinant organisms into the environment. d. Stringent safety rules make the use of recombinant DNA research possible. e. There is no danger involved in recombinant DNA research in bacteria.arrow_forwardWhich of the following is involved in recombinant DNA technology? Explain. MULTIPLE CHOICE. Choose only one: a. DNA polymerase b. DNA probes c. Restrition enzymes d. Reverse transcriptasearrow_forward
- Which of the following statements is accurate for DNA replication in your cells, but not PCR? a. DNA primers are required. b. DNA polymerase is stable at high temperatures. c. Ligase is essential. d. dNTPs are necessary.arrow_forwardPolymerase Chain Reaction, or PCR, can Group of answer choices A. target a specific region of DNA and cut it out of the rest of the genetic material for further analysis. B. copy the number of copies of a selected region of DNA linearly. C. increase the number of copies of a selected region of DNA exponentially. D. copy the entire genome at least a dozen times.arrow_forwardFor each situation, write the letter of the technique that would be most helpful; A. DNA editing A doctor wants to know if a patient has an inherited using CRISPR B. DNA replication using PCR C. DNA analysis through genetic testing D. DNA insertion 16. disorder. I A scientist needs many copies of a gene to conduct an 17. experiment. A genetic engineer wants to replace a defective copy of a gene with a functional copy in a chromosome. 18. into bacteria as a plasmid A medical researcher needs many copies of a protein 19. (insulin) to be produced to use in a medical treatment. A researcher crossed two purebred shrubs of the same species. One produces a fruit with a thin skin, and one produces a fruit with a thick skin. All of the plants resulting from the cross produce fruits with thick skins. Enter one letter in each blanks (19 & 20) to correctly complete the sentences. ninate Education TM, Inc.arrow_forward
- 1. How may recombinant DNA molecules be introduced into human cells? a. by splicing the needed genes into a mammalian chromosome using restriction enzymes. b. by adding plasmids to the mammalian cells. c. by using engineered viruses as vectors. d. by using a gene gun. 2. If someone is accused of a crime, investigators can collect his or her DNA to compare the DNA of the cells found at the crime scene. To collect human DNA, investigators often swab the inside of person’s cheek. Just a few human cheek cells contain enough material to perform PCR. In a cell, the nucleus and mitochondria contain DNA that is the starting material for PCR. Identify the 4 components needed to start a PCR reaction (equipment not included)arrow_forwardExplain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.arrow_forwardChoose the one answer that fits best. Which statement regarding PCR is NOT correct (videos)? a. PCR requires a copy of RNA that serves as a template b. Taq polymerase adds nucleotides to the primers and creates a complementary strand of DNA c. Annealing requires cooler temperatures than denaturation d. Repeated cycles of denaturation, annealing and extending DNA strands creates many identical copies of DNA e. PCR is a quick way of using minute quantities of DNA to create millions of copiesarrow_forward
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