Genetics: Analysis and Principles
Genetics: Analysis and Principles
5th Edition
ISBN: 9780073525341
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 7, Problem 1CONQ

The terms conjugation, transduction, and transformation are used to describe three different natural forms of genetic transfer between bacterial cells. Briefly discuss the similarities and differences among these processes.

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Summary Introduction

To review:

The similarities and differences among transformation, conjugation and transduction.

Introduction:

Genetic recombination is characterized by DNA (deoxyribonucleic acid) transfer in bacteria, from one organism, known as the donor, to another organism, known as the recipient. Such a genetic transfer is also known as horizontal gene transfer. In bacteria, obligate sexual reproduction is not present. Horizontal gene transfer is also known to occur between different species.

Explanation of Solution

The differences between transformation, conjugation and transduction are tabulated below.

Transduction Transformation Conjugation
The transfer of the genes from one bacterium to another is referred to as transduction. The incorporation of exogenous genetic material, resulting in the genetic alteration of a cell, is called transformation. The genetic material transfer among the bacterial species with the help of sex pilus is called conjugation.
It does not require for the occurrence of any physical contact between the recipient and the donor organism. It requires the occurrence of physical contact between the recipient and the donor organism. There is a direct cell-to-cell contact or a bridge-like connection between the two cells.
Resistant to DNase. Not resistant to DNase. Role of DNase is not defined.

The similarities among transformation, conjugation and transduction are as follows:

1. All three, that is, transformation, transduction and conjugation, are examples of horizontal gene transfer as genetic information is not transferred from the parent to the offspring.

2. All three methodsincrease genetic variation as a part of genetic material is transferred from the donor to the recipient.

Conclusion

Therefore, it can be concluded that all three, that is, transformation, transduction and conjugation are methods of transferring genetic material, but they all differ in the process of transfer.

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From one Escherichia coli strain, five Hfr strains were isolated. The location and orientation of the origin of transfer of each Hfr strain are shown in Figure 1. want to use these five strains to map the locus responsible for thiamine synthesis, called thi. Each Hfr strain is sensitive to rifampicin (RifS) and Thi+. Conjugation experiments are performed between each of the Hfr strains and an F- RifR Thi 9 leu 10 20 30 nadD pyrC trp 40 his 50 60 70 cysG 80 90 metA 100 Hfr1 Hfr2 Hfr3 Hfr4 Hfr5 Figure 1: Chromosome map of Escherichia coli. Five Hfr strains (Hfr1 to Hfr5) were isolated and the location and orientation of the origin of transfer is shown by the arrows in each Hfr strain. Distances in minutes are indicated. Leu: leucine biosynthesis; nadD: NAD biosynthesis; pyrC: pyrimidine biosynthesis; trp: tryptophan biosynthesis; his: histidine biosynthesis; cysG: cysteine biosynthesis; metA: biosynthesis of methionine. 1) What is the selection medium used in these conjugation…

Chapter 7 Solutions

Genetics: Analysis and Principles

Ch. 7.7 - Prob. 1COMQCh. 7.7 - Prob. 2COMQCh. 7.8 - Prob. 1COMQCh. 7 - 1. The terms conjugation, transduction, and...Ch. 7 - Prob. 2CONQCh. 7 - If you mix together an equal number of F+ and F...Ch. 7 - What is the difference between an F+ and an Hfr...Ch. 7 - 5. What is the role of the origin of transfer...Ch. 7 - 6. What is the role of sex pili during...Ch. 7 - Prob. 7CONQCh. 7 - Prob. 8CONQCh. 7 - Prob. 9CONQCh. 7 - 10. What is cotransduction? What determines the...Ch. 7 - Prob. 11CONQCh. 7 - Prob. 12CONQCh. 7 - Describe the steps that occur during bacterial...Ch. 7 - Prob. 14CONQCh. 7 - Prob. 15CONQCh. 7 - Antibiotics such as tetracycline, streptomycin,...Ch. 7 - What does the term complementation mean? If two...Ch. 7 - Prob. 18CONQCh. 7 - Prob. 19CONQCh. 7 - Prob. 1EQCh. 7 - 2. In the experiment of Figure 7.1, Lederberg and...Ch. 7 - Explain how a U-tube apparatus can distinguish...Ch. 7 - Prob. 4EQCh. 7 - 5. In a conjugation experiment, what is meant by...Ch. 7 - In your laboratory, you have an F strain of E....Ch. 7 - 7. As mentioned in question 2 of More Genetic...Ch. 7 - An Hfr strain that is hisE+ and pheA+ was mixed...Ch. 7 - Acridine orange is a chemical that inhibits the...Ch. 7 - Prob. 10EQCh. 7 - Prob. 11EQCh. 7 - Lets suppose a new strain of P1 phage has been...Ch. 7 - If two bacterial genes are 0.6 minute apart on the...Ch. 7 - 14. In a cotransduction experiment involving P1,...Ch. 7 - Prob. 15EQCh. 7 - Prob. 16EQCh. 7 - E17. If you took a pipette tip and removed a phage...Ch. 7 - Prob. 18EQCh. 7 - E19. In the experimental strategy described in...Ch. 7 - Prob. 20EQCh. 7 - Prob. 21EQCh. 7 - 1. Discuss the advantages of the genetic analysis...Ch. 7 - Complementation occurs when two defective alleles...
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Bacterial Genomics and Metagenomics; Author: Quadram Institute;https://www.youtube.com/watch?v=_6IdVTAFXoU;License: Standard youtube license