Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 5.4, Problem 2CC
Retrieve, Infer, Apply
What is a proteasome? Why is it important to the proper functioning of the ER?
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Increasing the saturation of the ammonium sulfate is a prerequisite in isolating a target protein that is rich in Cys and Tyr residues. Which of the following techniques should be considered in accurately quantifying the isolated protein?I. Running the isolated protein in a dialysis or GFC set up.II. Using Biuret or BCA assay as the colorimetric quantitation method.III. Using Bradford or Lowry assay as the colorimetric quantitation method.A. I onlyB. II onlyC. I and IIID. I, II and III. Bradford Assay is most suitable to use when the extraction buffer is below the target protein’s pI. This is so because the protein would be morea. Positively charged allowing the CBB G-250 dye to bind via its sulfonate groups.b. Negatively charged allowing the CBB G-250 dye to bind via its sulfonate groups.c. Neutrally charged allowing the CBB G-250 dye to bind via its sulfonate groups.d. Zwitterionic allowing the CBB G-250 dye to bind via its sulfonate groups.
Describe the bioreactor design consideration to be taken in account during the bioprocessing of animal cell culture. Please discuss which factors influence the choice of bioreactor design?
Using the biosynthesis of aminolevulinate (ALA) as an example, describe at
least two techniques that can be used to elucidate detail of a biosynthetic pathway.
Please keep brief - 5 sentences/dot points max.
Chapter 5 Solutions
Prescott's Microbiology
Ch. 5.1 - MICRO INQUIRY In addition to separating each...Ch. 5.3 - MICRO INQUIRY Which cytoskeletal filament is made...Ch. 5.3 - Prob. 1CCCh. 5.3 - Prob. 2CCCh. 5.3 - Prob. 3CCCh. 5.4 - Prob. 1MICh. 5.4 - Prob. 1CCCh. 5.4 - Retrieve, Infer, Apply What is a proteasome? Why...Ch. 5.4 - Prob. 3CCCh. 5.4 - Prob. 4CC
Ch. 5.4 - Describe the secretory pathway. To what...Ch. 5.4 - Prob. 6CCCh. 5.5 - MICRO INQUIRY Which subunit attaches to the rough...Ch. 5.5 - Prob. 1CCCh. 5.5 - Prob. 2CCCh. 5.5 - Prob. 3CCCh. 5.5 - Prob. 4CCCh. 5.6 - Prob. 1MICh. 5.6 - Describe the structure of aerobic mitochondria,...Ch. 5.6 - Prob. 2CCCh. 5.6 - Prob. 3CCCh. 5.7 - Prepare and label a diagram showing the detailed...Ch. 5.7 - Prob. 2CCCh. 5.7 - Prob. 3CCCh. 5.8 - Retrieve, Infer, Apply Outline the major...Ch. 5.8 - Retrieve, Infer, Apply What characteristics make...Ch. 5 - Prob. 1RCCh. 5 - Prob. 2RCCh. 5 - Prob. 3RCCh. 5 - Prob. 4RCCh. 5 - Discuss the statement: The most obvious difference...Ch. 5 - Prob. 2ALCh. 5 - Compare the mechanisms by which most eukaryotic...Ch. 5 - Prob. 4AL
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