The reason corresponding to the fact that Taq polymerase is especially useful for PCR is to be stated. Concept introduction: The reaction that is used to make the duplicates of a particular DNA segment is known polymerase chain reaction (PCR). This reaction forms millions duplicate copies of a specific DNA segment. The enzyme Taq polymerase is a synthetic enzyme. It is used to generate DNA strands at a very fast rate than other polymerases enzymes.

Question

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Answer 1

Question

Chapter 5, Problem 1P

Interpretation Introduction

Interpretation:

The reason corresponding to the fact that Taq polymerase is especially useful for PCR is to be stated.

Concept introduction:

The reaction that is used to make the duplicates of a particular DNA segment is known polymerase chain reaction (PCR). This reaction forms millions duplicate copies of a specific DNA segment.

The enzyme Taq polymerase is a synthetic enzyme. It is used to generate DNA strands at a very fast rate than other polymerases enzymes.

Expert Solution & Answer

Answer to Problem 1P

The enzyme, Taq polymerase, is especially useful for PCR because this enzyme is stable in the high temperature condition also and does not get denatured.

Explanation of Solution

The polymerase chain reaction (PCR) possesses the high temperature due to which the natural or other polymerases enzymes get destroyed.

The enzyme, Taq polymerase, is a heat-stable enzyme. It is one of the types of DNA polymerase that can be stable at the high temperature also. This enzyme is stable in the condition of protein-denaturing as well.

Thus, Taq polymerase is especially useful for polymerase chain reaction which takes place at high temperature.

Conclusion

The enzyme, Taq polymerase, is especially useful for PCR because this enzyme is stable in the high temperature condition also and does not get denatured.

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Pick a plasmid . What was its approximate transformation? Express it in # colonies per microgram of DNA transformed. Assume the original DNA was about .001 ug/ul . Count how many colonies you got on one plate (or estimate that number) and figure out how much of the total solution you plated on that plate. Multiply by all the plates, if you plated all of it. OR, if you only plated some of it, figure out how many colonies you would have gotten had you plated all of it. Divide by the number of ug used.

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Answer 2

Question

Chapter 5, Problem 1P

Interpretation Introduction

Interpretation:

The reason corresponding to the fact that Taq polymerase is especially useful for PCR is to be stated.

Concept introduction:

The reaction that is used to make the duplicates of a particular DNA segment is known polymerase chain reaction (PCR). This reaction forms millions duplicate copies of a specific DNA segment.

The enzyme Taq polymerase is a synthetic enzyme. It is used to generate DNA strands at a very fast rate than other polymerases enzymes.

Expert Solution & Answer

Answer to Problem 1P

The enzyme, Taq polymerase, is especially useful for PCR because this enzyme is stable in the high temperature condition also and does not get denatured.

Explanation of Solution

The polymerase chain reaction (PCR) possesses the high temperature due to which the natural or other polymerases enzymes get destroyed.

The enzyme, Taq polymerase, is a heat-stable enzyme. It is one of the types of DNA polymerase that can be stable at the high temperature also. This enzyme is stable in the condition of protein-denaturing as well.

Thus, Taq polymerase is especially useful for polymerase chain reaction which takes place at high temperature.

Conclusion

The enzyme, Taq polymerase, is especially useful for PCR because this enzyme is stable in the high temperature condition also and does not get denatured.

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Answer 3

Question

Chapter 5, Problem 1P

Interpretation Introduction

Interpretation:

The reason corresponding to the fact that Taq polymerase is especially useful for PCR is to be stated.

Concept introduction:

The reaction that is used to make the duplicates of a particular DNA segment is known polymerase chain reaction (PCR). This reaction forms millions duplicate copies of a specific DNA segment.

The enzyme Taq polymerase is a synthetic enzyme. It is used to generate DNA strands at a very fast rate than other polymerases enzymes.

Expert Solution & Answer

Answer to Problem 1P

The enzyme, Taq polymerase, is especially useful for PCR because this enzyme is stable in the high temperature condition also and does not get denatured.

Explanation of Solution

The polymerase chain reaction (PCR) possesses the high temperature due to which the natural or other polymerases enzymes get destroyed.

The enzyme, Taq polymerase, is a heat-stable enzyme. It is one of the types of DNA polymerase that can be stable at the high temperature also. This enzyme is stable in the condition of protein-denaturing as well.

Thus, Taq polymerase is especially useful for polymerase chain reaction which takes place at high temperature.

Conclusion

The enzyme, Taq polymerase, is especially useful for PCR because this enzyme is stable in the high temperature condition also and does not get denatured.

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Answer 4

Question

Chapter 5, Problem 1P

Interpretation Introduction

Interpretation:

The reason corresponding to the fact that Taq polymerase is especially useful for PCR is to be stated.

Concept introduction:

The reaction that is used to make the duplicates of a particular DNA segment is known polymerase chain reaction (PCR). This reaction forms millions duplicate copies of a specific DNA segment.

The enzyme Taq polymerase is a synthetic enzyme. It is used to generate DNA strands at a very fast rate than other polymerases enzymes.

Expert Solution & Answer

Answer to Problem 1P

The enzyme, Taq polymerase, is especially useful for PCR because this enzyme is stable in the high temperature condition also and does not get denatured.

Explanation of Solution

The polymerase chain reaction (PCR) possesses the high temperature due to which the natural or other polymerases enzymes get destroyed.

The enzyme, Taq polymerase, is a heat-stable enzyme. It is one of the types of DNA polymerase that can be stable at the high temperature also. This enzyme is stable in the condition of protein-denaturing as well.

Thus, Taq polymerase is especially useful for polymerase chain reaction which takes place at high temperature.

Conclusion

The enzyme, Taq polymerase, is especially useful for PCR because this enzyme is stable in the high temperature condition also and does not get denatured.

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Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Chapter 5, Problem 1P

Interpretation Introduction

Interpretation:

The reason corresponding to the fact that Taq polymerase is especially useful for PCR is to be stated.

Concept introduction:

The reaction that is used to make the duplicates of a particular DNA segment is known polymerase chain reaction (PCR). This reaction forms millions duplicate copies of a specific DNA segment.

The enzyme Taq polymerase is a synthetic enzyme. It is used to generate DNA strands at a very fast rate than other polymerases enzymes.

Expert Solution & Answer

Answer to Problem 1P

The enzyme, Taq polymerase, is especially useful for PCR because this enzyme is stable in the high temperature condition also and does not get denatured.

Explanation of Solution

The polymerase chain reaction (PCR) possesses the high temperature due to which the natural or other polymerases enzymes get destroyed.

The enzyme, Taq polymerase, is a heat-stable enzyme. It is one of the types of DNA polymerase that can be stable at the high temperature also. This enzyme is stable in the condition of protein-denaturing as well.

Thus, Taq polymerase is especially useful for polymerase chain reaction which takes place at high temperature.

Conclusion

The enzyme, Taq polymerase, is especially useful for PCR because this enzyme is stable in the high temperature condition also and does not get denatured.

Answer 6

Chapter 5, Problem 1P

Interpretation Introduction

Interpretation:

The reason corresponding to the fact that Taq polymerase is especially useful for PCR is to be stated.

Concept introduction:

The reaction that is used to make the duplicates of a particular DNA segment is known polymerase chain reaction (PCR). This reaction forms millions duplicate copies of a specific DNA segment.

The enzyme Taq polymerase is a synthetic enzyme. It is used to generate DNA strands at a very fast rate than other polymerases enzymes.

Expert Solution & Answer

Answer to Problem 1P

The enzyme, Taq polymerase, is especially useful for PCR because this enzyme is stable in the high temperature condition also and does not get denatured.

Explanation of Solution

The polymerase chain reaction (PCR) possesses the high temperature due to which the natural or other polymerases enzymes get destroyed.

The enzyme, Taq polymerase, is a heat-stable enzyme. It is one of the types of DNA polymerase that can be stable at the high temperature also. This enzyme is stable in the condition of protein-denaturing as well.

Thus, Taq polymerase is especially useful for polymerase chain reaction which takes place at high temperature.

Conclusion

The enzyme, Taq polymerase, is especially useful for PCR because this enzyme is stable in the high temperature condition also and does not get denatured.

Answer 7

Chapter 5, Problem 1P

Interpretation Introduction

Interpretation:

The reason corresponding to the fact that Taq polymerase is especially useful for PCR is to be stated.

Concept introduction:

The reaction that is used to make the duplicates of a particular DNA segment is known polymerase chain reaction (PCR). This reaction forms millions duplicate copies of a specific DNA segment.

The enzyme Taq polymerase is a synthetic enzyme. It is used to generate DNA strands at a very fast rate than other polymerases enzymes.

Answer 8

Expert Solution & Answer

Answer to Problem 1P

The enzyme, Taq polymerase, is especially useful for PCR because this enzyme is stable in the high temperature condition also and does not get denatured.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Explanation of Solution

The polymerase chain reaction (PCR) possesses the high temperature due to which the natural or other polymerases enzymes get destroyed.

The enzyme, Taq polymerase, is a heat-stable enzyme. It is one of the types of DNA polymerase that can be stable at the high temperature also. This enzyme is stable in the condition of protein-denaturing as well.

Thus, Taq polymerase is especially useful for polymerase chain reaction which takes place at high temperature.

Answer 12

Conclusion

The enzyme, Taq polymerase, is especially useful for PCR because this enzyme is stable in the high temperature condition also and does not get denatured.

Answer 13

Ch. 5 - Prob. 1P Ch. 5 - Prob. 2P Ch. 5 - Prob. 3P Ch. 5 - Prob. 4P Ch. 5 - Prob. 5P Ch. 5 - Prob. 6P Ch. 5 - Prob. 7P Ch. 5 - Prob. 8P Ch. 5 - Prob. 9P Ch. 5 - Prob. 10P

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