Campbell Biology in Focus (2nd Edition)
2nd Edition
ISBN: 9780321962751
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece
Publisher: PEARSON
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Chapter 4, Problem 9TYU
SCIENTIFIC INQUIRY In studying micrographs of an unusual protist (single-celled eukaryote) that you found in a sample of pond water, you spot an organelle that you can’t recognize. You successfully develop a method for growing this organism in liquid in the laboratory. Describe how you would go about finding out what this organelle is and what it does in the cell. Assume that you would make use of additional microscopy, cell fractionation, and biochemical tests.
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Which electron or light microscopy technique would you use for the following? (also explain)
For visualizing the fine structure of cellular organelles and cell architecture in the cell interior using very thin cross-sections made in Epon.
To obtain sequential images of a macromolecule in order to study its activity in real-time.
To obtain an extremely clear three-dimensional image of the nucleus of a single cell.
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Items (6 items) (Drag and drop into the appropriate area below)
Take movies of
unmanipulated
cells swimming
or crawling in a
drop of water.
Categories
View three-
dimensional
images of the
outer surface of a
paramecium,
including cilia.
bright-field
Drag and drop here
View cytoplasmic
streaming
(movement of
organelles within
the cytoplasm) in
a paramecium.
fluorescence
Drag and drop here
Stain and
visualize
chromosomes
and mitotic
spindles in
dividing
amoebae.
Visualize
ribosomes in the
cytosol and
mitochondria of
amoebas.
scanning electron
Drag and drop here
Use labeled
antibodies to
locate and make
color images of
lysosomes in
both…
Scanning electron microscope can be used for ()
A. Observe images of different sections of cells
B. Observe living cells
C. Quantitative analysis of chemical composition in cells
D. Observation of cell surface stereomorphology
E. Observation of cellular activity
Chapter 4 Solutions
Campbell Biology in Focus (2nd Edition)
Ch. 4.1 - Prob. 1CCCh. 4.1 - Prob. 2CCCh. 4.2 - Briefly describe the structure and function of the...Ch. 4.2 - Prob. 2CCCh. 4.3 - What role do ribosomes play in carrying out...Ch. 4.3 - Describe the molecular composition of nucleoli,...Ch. 4.3 - WHAT IF? As a cell begins the process of dividing,...Ch. 4.4 - Describe the structural and functional...Ch. 4.4 - Describe how transport vesicles integrate the...Ch. 4.4 - WHAT IF? Imagine a protein that functions in the...
Ch. 4.5 - Describe two characteristics shared by...Ch. 4.5 - Prob. 2CCCh. 4.5 - Prob. 3CCCh. 4.6 - Prob. 1CCCh. 4.6 - WHAT IF? Males afflicted with Kartageners syndrome...Ch. 4.7 - In what way are the cells of plants and animals...Ch. 4.7 - Prob. 2CCCh. 4.7 - MAKE CONNECTIONS The polypeptide chain that makes...Ch. 4 - Which structure is not part of the endomembrane...Ch. 4 - Which structure is common to plant and animal...Ch. 4 - Which of the following is present in a prokaryotic...Ch. 4 - Prob. 4TYUCh. 4 - Cyanide binds to at least one molecule involved in...Ch. 4 - What is the most likely pathway taken by a newly...Ch. 4 - Which cell would be best for studying lysosomes?...Ch. 4 - DRAW IT From memory, draw two eukaryotic cells....Ch. 4 - SCIENTIFIC INQUIRY In studying micrographs of an...Ch. 4 - FOCUS ON EVOLUTION Compare different aspects of...Ch. 4 - FOCUS ON ORGANIZATION Considering some of the...Ch. 4 - Prob. 12TYU
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- The following fluorescence microscopy shows several cells. What part of the cell is labelled by the fluorescence here? b The Cytosol The Endoplasmic Reticulum The nucleolus The nucleus The actin cytoskeletonarrow_forwardAnimal Cell (Ideal Model) This is a model of an ideal animal cell. Objects are not to scale and many of them would not be visible with a compound light microscope. Fill in the blanks.arrow_forwardPick the best form of microscopy to use in each situation, each answer used only once: You want to figure out if a bacterium can ✓ [Choose ] swim in a liquid medium You want to visualize the repeating crystalline structure of the S-layer You want to identify the basic cell wall structure of an unknown bacterium You want to watch proteins of the divisome to see how they change their location in dividing cells You want to visualize a single membrane protein complex on the surface of a cell Atomic Force Microscopy Phase-contrast microscopy Light microscopy (with staining) Fluorescence microscopy Electron microscopy [Choose] [Choose] [Choose ]arrow_forward
- Which of the following microscopes typically requires the use of vital dyes (like methylene blue) to visualize large subcellular structures in a living cell (like the nucleolus or the mitochondrion)? the scanning/tunneling electron microscope (STEM), with good resolution up to about 100,000,000x the transmission electron microscope (TEM), with good resolution up to about 100,000x the scanning electron microscope (SEM), with good resolution up to about 1,000,000x the compound light microscope, with good resolution up to about 1,500x all of the above microscopes would be equally useful in visualizing the interior of organellesarrow_forwardAnton Van Leeuwenhoek observed under the microscope a drop of water and sawmoving unicellular microorganisms. How can he prove that these are also livingorganisms since they cannot be seen by the naked eye? Provide a brief explanation as to its relationship with the principle of cell theoryinvolved. Thank youarrow_forwardBriefly describe how magnetic bead microrheometry is used to measure the mechanical properties of cells.arrow_forward
- PART D: CALCULATING THE ACTUAL SIZE OF A CELL. Once you know your microscope's diameter of the field of view, you can now calculate the actual size of your specimen. The ACTUAL SIZE of the specimen is the real size of the cell you are looking at. When you look at the cells through the microscope. the cells look very tiny. It is possible to determine the actual size of the cells you are looking at under LP.MP and HP. We will determine the actual size of a cell u I under HP. Step 1- Look at the cells under HP MAG. Step 2- Pick one cell you want to know the size of. Step 3- Imagine that this cell is placed along the left side of the field of view. (see example) Step 4-Estimate how many cels could fit across the field of view. Step 5- Record your answer Step 6- Use the formula below to calculate the actual size of your cell NOTE: Cells are very tiny therefore; the size of a cell is calculated in micrometers (um) and not mm. Cell size in (mm) ACTUAL SIZE OF CELL= HIGH POWERFOV.diameter of…arrow_forwardYou are using a computer program to design a new single-celled organism. Discuss why a flat, plate-like cell will be more efficient in transporting materials than a spherical, ball-like cell of the same volume.arrow_forwardMany microscopy techniques use cell characteristics, such as differences in absorption, refraction, or even light polarization. Fluorescence microscopy provides an image from a single molecular species, simply by looking at the fluorescence emission. How does fluorescence microscopy compare to other techniques? A.Image the location, diffusion or even transport of intracellular molecules.B.Accurately calculate the cytosol refractive index of a cellC.Count the number of cellsD.Accurately measure kernel sizearrow_forward
- What differences are there between a free-living, single-celled organism such as a paramecium and a single human cell such as a ciliated cell of the respiratory tract? Use cited.arrow_forwardUsing the appropriate osmotic terms (hypertonic, hypotonic, or isotonic) describe what would happen to each organism in the following settings: A single-celled freshwater protist is placed into a beaker of salt water. A salt-water snail is mistakenly put into a freshwater tank. A head of lettuce is placed soaked in a sink of salt water. A bunch of carrots are placed soaked a sink of distilled water.arrow_forwardPART D: CALCULATING THE ACTUAL SIZE OF A CELL. Once you know your microscope's diameter of the field of view, you can now calculate the actual size of your specimen. t you The ACTUAL SIZE of the specimen is the real size of the cell you are looking at. When you look at the cells through the microscope, the cells look very tiny. It is possible to determine the actual size of the cells you are looking at under LP.MP and HP. We will determine the actual size of a cell under HP. Step 1- Look at the cells under HP MAG. Step 2- Pick one cell you want to know the size of. Step 3- Imagine that this cell is placed along the left side of the field of view. (see example) Step 4-Estimate how many cels could fit across the field of view. 5- Record your answer Step 6- Use the formula below to calculate the actual size of your cell NOTE: Cells are very tiny therefore; the size of a cell is calculated in micrometers (um) and not mm. Cell size in (mm) ACTUAL SIZE OF CELL= HIGH POWERFOV.diameter of…arrow_forward
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