Microbiology: An Introduction
11th Edition
ISBN: 9780321733603
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: Benjamin Cummings
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Chapter 3, Problem 9R
Fill in the following table regarding the
| Appearance After This Step | |||
| Steps | Gram-Positive Cells | Gram-Negative Cells | |
| Crystal violet | a. __________ | e. __________ | |
| Iodine | b. __________ | f. __________ | |
| Alcohol-acetone | c. __________ | q. __________ | |
| Safranin | d. __________ | h. __________ | |
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4.
You find that all four flower color genes map to the second
chromosome, and perform complementation tests with deletions for each
gene. You obtain the following results:
(mutant a = blue, mutant b = white, mutant c = pink, mutant d = red)
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Results of Complementation tests
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Jostum
Mutant a
b
с
Del (2.2 -2.6)
blue
white
pink
purple
Del (2.3-2.8)
blue
white
pink
red
Del (2.1 -2.5)
blue
purple
pink
purple
Del (2.4-2.7)
purple
white
pink
red
C
d
Indicate where each gene maps:
a
b
ori ai indW (anioq 2) .8
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1. Below is a pedigree of a rare trait that is associated with a variable number
repeat. PCR was performed on individuals using primers flanking the VNR,
and results are shown on the agarose gel below the pedigree.
I.1 1.2 II.1 II.2 II.3 II.4 II.5 II.6 11.7 III.1 III.2 III.3 III.4etum
A. (5 points) What is the mode of inheritance?
B. (10 points) Fill in the expected gel lanes for II.1, II.5, III.2, III.3 and III.4
C. (5 points) How might you explain the gel results for II.4?
To study genes that create the purple flower color in peas, you isolate 4 amorphic mutations. Each results in a flower with a different color, described
mutant a = blue
mutant c = pink
mutant b = white
mutant d = red
A. In tests of double mutants, you observe the following phenotypes:
mutants a and b = blue
mutants b and c = white
mutants c and d = pink
Assuming you are looking at a biosynthetic pathway, draw the pathway indicating which step is affected by each mutant.
B. What is the expected flower color of a double mutant of a and c?
Chapter 3 Solutions
Microbiology: An Introduction
Ch. 3 - Fill in the following blanks. 1. 1 m = ________ m...Ch. 3 - Prob. 2RCh. 3 - Prob. 3RCh. 3 - Prob. 4RCh. 3 - Prob. 5RCh. 3 - Why is a mordant used in the Gram stain? In the...Ch. 3 - Prob. 7RCh. 3 - Prob. 8RCh. 3 - Fill in the following table regarding the Gram...Ch. 3 - NAME IT A sputum sample from Calle, a 30-year-old...
Ch. 3 - Prob. 1ACh. 3 - Prob. 2ACh. 3 - Why isnt the Gram stain used on acid-fast...Ch. 3 - Endospores can be seen as refractile structures in...Ch. 3 - Through the microscope, the green structures are...Ch. 3 - Prob. 2MCQCh. 3 - Carbolfuchsin can be used as a simple stain and a...Ch. 3 - Prob. 4MCQCh. 3 - Which of the following is not a functionally...Ch. 3 - Which of the following pairs is mismatched? 1....Ch. 3 - Assume you stain Clostridium by applying a basic...Ch. 3 - Prob. 8MCQCh. 3 - In 1996, scientists described a new tapeworm...Ch. 3 - Prob. 10MCQCh. 3 - In 1882, German bacteriologist Paul Erhlich...Ch. 3 - Laboratory diagnosis of Neisseria gonorrhoeae...Ch. 3 - Assume that you are viewing a Gram-stained sample...
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