Biochemistry
8th Edition
ISBN: 9781464126109
Author: Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr., Lubert Stryer
Publisher: W. H. Freeman
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Chapter 3, Problem 14P
Interpretation Introduction
Interpretation:
The method in which the antibody-bound beads can be used for the purification of protein should be determined.
Concept introduction:
Affinity chromatography can be defined as a technique of isolating the biochemical mixture. The biochemical mixture is based on an extremely definite interaction between antigen and antibody, receptor and ligand, enzyme and substrate, or protein and
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Chapter 3 Solutions
Biochemistry
Ch. 3 - Prob. 1PCh. 3 - Prob. 2PCh. 3 - Prob. 3PCh. 3 - Prob. 4PCh. 3 - Prob. 5PCh. 3 - Prob. 6PCh. 3 - Prob. 7PCh. 3 - Prob. 8PCh. 3 - Prob. 9PCh. 3 - Prob. 10P
Ch. 3 - Prob. 11PCh. 3 - Prob. 12PCh. 3 - Prob. 13PCh. 3 - Prob. 14PCh. 3 - Prob. 15PCh. 3 - Prob. 16PCh. 3 - Prob. 17PCh. 3 - Prob. 18PCh. 3 - Prob. 19PCh. 3 - Prob. 20PCh. 3 - Prob. 21PCh. 3 - Prob. 22PCh. 3 - Prob. 23PCh. 3 - Prob. 24PCh. 3 - Prob. 25PCh. 3 - Prob. 26PCh. 3 - Prob. 27PCh. 3 - Prob. 28PCh. 3 - Prob. 29P
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- Predict the number of bands and apparent mol. wt. of the following proteins on SDS gels. 1. Immunoglobulin G in a non-reducing gel (no β-mercaptoethanol added in the sample solution) – the light chains have a molecular weight of 25,000 Da and the heavy chains 50,000 Da.arrow_forwardMonoclonal antibodies can be conjugated to an insoluble support by chemical methods. Explain how these antibody-bound beads can be exploited for protein purification.arrow_forwardBiomedical Engineering: An equilibrium dialysis experiment was performed to characterize the binding affinity of a mouse IgG or human IgG antibody for an antigen, GAD65. (The association constant for the monoclonal mouse IgG is 4.75 x 10^8 M-1 and for a human IgG is 1.3 x 10^10 M-1) a- calculate the dissociation constant for each antibody and explain which antibody has a higher binding affinity for GAD65? b-calculate the fraction of free Ab sites for each antibody for a For a GAD65 concentration of 0.7 nMarrow_forward
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- Affinity purification: Assemble the following components to set up affinity chromatography to purify a protein. Which protein do the bound protein from the column? you think is purified in this affinity purification type? Which compound is used to elute Glutathione Sepharose Column Bead Spacer (or arm) GST- tagged proteinarrow_forwardPhysical therapy and low level laser therapy. How LLLT can be used in this practice ? Has it been used in this practice? Would it be beneficial?arrow_forwardExample. A new plant species was discovered. To determine the protein content of the plant, an adequate amount of the plant extract was acquired, and bradford assay was performed. The absorbance reading of the plant extract is 0.272. What is the protein concentration, in μg/L, of the plant extract? A bovine serum albumin (BSA) Stock solution with a concentration of 250 μg/mL was used and mixtures with the following compositions and absorbance reading were prepared in picture.arrow_forward
- In SDS-PAGE. What chemical is used to ensure that all protein molecules are coated with a negative charge? IPTG. β-mercaptoethanol. X-Gal. SDS.arrow_forwardProteins that defend the body. Explain the mechanismarrow_forwardignment/takeCovalentActivity.do?locator%3Dassignment-take [Review Topics] [References] Use the References to access important values if needed for this question. The protein pepsin has an isoelectric point of 0.800. Suppose you did a pH titration of a solution containing pepsin. At what pH will the protein aggregate? pH = %3D Submit Answer Retry Entire Group 9 more group attempts remaining Previous Email Instructorarrow_forward
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