Campbell Biology
Campbell Biology
12th Edition
ISBN: 9780135188743
Author: Urry
Publisher: PEARSON
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Chapter 28.3, Problem 4CC
Summary Introduction

To summarize: How O2 (oxygen) and CO2 (carbon dioxide) are used and produced by the aerobic algae and by the chlorarachniophytes with the help of the figures, Refer Fig.9.2 "Energy flow and chemical recycling in ecosystems", and Fig.10.6 "An overview of photosynthesis: Cooperation of the light reactions and the Calvin cycle” in the textbook.

Concept introduction: Chlorarachniophytes comprises of a small group of the cercozoans. These possess secondary endosymbiosis and are mixotrophic. These organisms ingest small protists and also perform photosynthesis. Aerobic algae also perform the process of photosynthesis and cellular respiration.

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Why is an isotonic buffered extraction medium used to isolate the chloroplasts? 1.To gently lyse the chloroplasts, leaving the electron transport membranes intact, whilst allowing DCPIP access 2.To maintain a constant acidic pH and favourable hyper-osmotic conditions, to ensure that the chloroplasts remained intact and functional during extraction 3.To gently separate the chloroplasts from each other, leaving the electron transport membranes intact, whilst allowing DCPIP access 4.To maintain a constant physiological pH and favourable iso-osmotic conditions, to ensure that the chloroplasts remained intact and functional during extraction.
Why is it necessary to lyse the chloroplasts? 1.DCPIP passes easily across the intact chloroplast membrane and the thylakoid membrane, so electron transport could not be reliably measured 2.DCPIP passes easily across the intact chloroplast membrane, but can't get into the thylakoid, so electron transport could not be reliably measured 3.DCPIP cannot easily pass across the intact chloroplast membrane, so electron transport across the thylakoid membrane could not be reliably measured
Discuss: 1) the mechanisms that transport photosynthate from sources to sinks.  Why is this important on a physiological level and a societal level?

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