Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 28, Problem 2MCQ
Acetobacter is necessary for only one of the steps of vitamin C manufacture. The easiest way to accomplish this step would be to
- a. add substrate and Acetobacter to a test tube.
- b. affix Acetobacter to a surface and run substrate over it.
- c. add substrate and Acetobacter to a bioreactor.
- d. find an alternative to this step.
- e. none of the above
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To retrieve a sample from a culture tube with an inoculating loop, the cap of the tube is a. removed and held in one’s teeth. b. removed and held with the fingers of the loop hand. c. removed with the fingers of the loop hand and placed in the fingers of the tube hand. d. removed with the fingers of the loop hand and placed on the laboratory bench. e. Any of these methods can be used.
MacConkey (MAC) Agar is used to isolate gram-negative enteric bacteria.Peptone provides nutrients necessary for growth.Bile salts and crystal violet inhibit the growth of gram positive bacteria.Lactose is a sugar. When metabolized, colonies turn the medium pink.Agar is a solidifying agent.Which component/s make the MAC agar selective?
a. Lactose
b. Peptone
c. Agar
d. Bile salts & Crystal Violet
What is the best descriptive match for the following culture media?
A. Specific biological characteristics allow this media to select specific groups of bacteria.
B. All the components are well known.
C. Will contain components that can inhibit certain groups of bacteria.
D. Can be used to grow difficult growing or nutritional complex bacteria.
E. This media contains amino acids for growing bacteria.
select
1. Synthetic media
select - 2. Selective media
select - 3. Enriched media
select
4. Differential media
select - 5. Complex media
Chapter 28 Solutions
Microbiology: An Introduction
Ch. 28 - What is industrial microbiology? Why is it...Ch. 28 - How does commercial sterilization differ from...Ch. 28 - Why is a can of blackberries preserved by...Ch. 28 - Outline the steps in the production of cheese, and...Ch. 28 - Beer is made with water, malt, and yeast; hops are...Ch. 28 - Why is a bioreactor better than a large flask for...Ch. 28 - The manufacture of paper includes the use of...Ch. 28 - Describe an example of bioconversion. What...Ch. 28 - Prob. 9RCh. 28 - NAME IT Van Leeuwenhoek was the first to see this...
Ch. 28 - Foods packed in plastic for microwaving are a....Ch. 28 - Acetobacter is necessary for only one of the steps...Ch. 28 - Use the following choices to answer questions 35:...Ch. 28 - The spoilage of canned foods caused by Geobacillus...Ch. 28 - A heat-resistant fungus that causes spoilage in...Ch. 28 - The term 12D treatment refers to a. heat treatment...Ch. 28 - Which one of the following is not a fuel produced...Ch. 28 - Which type of radiation is used to preserve foods?...Ch. 28 - Which of the following reactions is undesirable in...Ch. 28 - Which of the following reactions is an oxidation...Ch. 28 - Which bacteria seem to be most frequently used in...Ch. 28 - Methylophilus methylotrophus can convert methane...Ch. 28 - Faded worn-look denim is produced with cellulase....Ch. 28 - Suppose you are culturing a microorganism that...Ch. 28 - Researchers at the CDC inoculated apple cider with...Ch. 28 - The antibiotic efrotomycin is produced by...
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- Your nasal sample showed colonies with a clear halo around them on blood agar. What can you determine based on that observation? a. The organism is a mannitol fermenter b. The organism is able to utilize starch c. The organism is reducing the hemoglobin and damaging the erythrocytes d. The organism is beta-hemolytic e. The organism is unable to use red blood cells as a nutrient sourcearrow_forwardWhy is Phenol red being utilized in MacConkey agar? Choose among the choices and further explain it.A. Inhibit microscopic organismsB. Detect the production of gas by-productsC. Conduct the sulfate reduction testD. Detect the acid reaction of lactose fermentationarrow_forwardSelect all of the pathways that can lead to pore production in a microbial cell. A. Alternative B. Lectin C. Classicalarrow_forward
- Based on the growth curve, which ONE of the following best describes what would happen if sample of culture in the presence of drug B is inoculated onto drug-free growth medium? Select one: A. Growth present, but cell count increased by at least 99.9% of the original culture B. No growth after 24 hrs C. Growth at 24 hrs D. Not possible to predict because the culture could either be alive or dead E. Growth present, but cell count reduced by at least 99.9% of the original culturearrow_forwardDescribe how each of the following would appear when grown on EMB agar: a. Escherichia coli b. Pseudomonas aeruginosarrow_forwardCreate a flow chart on how to prepare a nutrient agararrow_forward
- Lysine decarboxylase media is yellow after incubating an organism in it at 37 degrees for 24 hours. Which of the following can be interpreted from this result? A. The organism did not grow so lysine decarboxylase activity could not be determined. B. The organism is negative for lysine decarboxylase. C. The media is too acidic to determine lysine decarboxylase activity. D. The organism is positive for lysine decarboxylasearrow_forwardA. Antibiotics, such as gentamicin, are used in media to prevent growth of susceptible microbes. Is gentamicin media a selective, differential or enrichment media? B. Sorbitol is a sugar alcohol that all Escherichia bacteria can normally ferment into an acidic waste compound. This acidic end product is detected by including a pH indicator in the agar and noticing a color change. The strain of Escherichia that is a common food pathogen, E. coli O157:H7, however can not ferment sorbitol. It will grow similar to other E. coli, but not change the agar color. Is sorbitol in nutrient agar a selective, differential or enrichment media?arrow_forwardAhmad has prepared a seed culture containing recombinant Escherichia coli for the production of lipase enzyme. The seed culture was incubated for 18 hours before inoculating 5% of the seed culture into 2L of production media. a. Predict the consequences if the seed culture has been contaminated. b. Discuss whether the seed culture is meeting the criteria as inoculum or not. asap please.arrow_forward
- If you streak a sample from your nose onto a Mannitol salt agar plate and the medium turns yellow, what does that tell you? (choose all that apply) a. The organism is a yeast b. The organism ferments mannitol c. The organism is beta-hemolytic d. The organism is likely Staphylococus aureus e. The organism is acid tolerantarrow_forwardMacConkey Agar is considered as: a. Differential CM only b. Enriched CM only c. Selective CM only d. Selective and differential CMarrow_forwardthis is multipartarrow_forward
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