Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Textbook Question
Chapter 27, Problem 3TY
- a. determine if a bacterial strain is a pathogen.
- b. determine if a sample of bacteria sample can break down oil.
- c. infer the structure of the cell wall of bacteria and their response to antibiotics.
- d. count bacteria in medical or environmental samples.
- e. do all of the above.
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Students have asked these similar questions
What is the purpose of diluting a culture by making "quadrant streaks" (or "streak plating") bacteria, and what can we use them for? Check all that apply
A.
To observe colony morphology/color
B.
To isolate viral plaques
C.
testing growth conditions or treatments (such as media, antibiotics, incubation temperature etc.,)
D.
To isolate a clonal population of bacteria from the rest of the culture
E.
For subculturing (transferring to new media so we can grow more bacteria, storing the plate in a fridge for repeated use so the bacteria don't die quickly)
F.
Accurate quantification (to calculate CFU/mL)
G.
To know what strain of bacteria you're working with, since you can tell based on how it looks on the plate.
H.
To lower risk of contamination/identify contamination
Attached below is the study about the effects of handwashing and the effectiveness of soap against bacteria. What conclusions can be generated from this result? (see rubrics
for the point marking)
A before washing hands
C
10 second hand wash
B
D
hot water only rinse
30 second hand wash
Figure 8: Agar plates with bacteria A) before washing hands B) after rinsing hands in hot water C) After washing
hands with soap for 10 seconds and D) after washing hands for 30 seconds. Images show that the number of
bacteria on the plate are not greatly reduced after a hot water rinse or 10 second handwash but bacterial levels are
reduced after a proper hand wash for 30 seconds.
You make a smear of E. coli and then simple stain the slide with methylene blue. If you failed to use correct aseptic technique by not sterilizing the loop before picking up the bacterial sample for the smear, what results may be seen under the microscope?
A
Both blue and purple cells may be seen on the slide.
B
No cells may be visible on the slide.
C
There may be cells of different shapes, sizes, and arrangements.
Chapter 27 Solutions
Biology
Ch. 27.2 - Core Skill: Connections Look ahead to Section...Ch. 27.2 - Prob. 2CSCh. 27.2 - Structure and Movement Concept Check: Does the...Ch. 27.2 - Prob. 3CSCh. 27.3 - Prob. 1CCCh. 27.5 - Prob. 1EQCh. 27.5 - Prob. 2EQCh. 27 - Which of the following features is common to...Ch. 27 - The bacterial phylum whose members typically...Ch. 27 - Gram-staining is a procedure that microbiologists...
Ch. 27 - Place the following steps in the correct order,...Ch. 27 - What ecological role do cyanobacteria play? a....Ch. 27 - Prob. 6TYCh. 27 - Prob. 7TYCh. 27 - Prob. 8TYCh. 27 - Prob. 9TYCh. 27 - Prob. 10TYCh. 27 - Prob. 1CQCh. 27 - Prob. 2CQCh. 27 - Prob. 3CQCh. 27 - Prob. 1COQCh. 27 - Prob. 2COQ
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Attached below is the study about the effects of handwashing and the effectiveness of soap against bacteria. What conclusions can be generated from this result? A C before washing hands 10 second hand wash B D hot water only rinse 30 second hand wash Figure 8: Agar plates with bacteria A) before washing hands B) after rinsing hands in hot water C) After washing hands with soap for 10 seconds and D) after washing hands for 30 seconds. Images show that the number of bacteria on the plate are not greatly reduced after a hot water rinse or 10 second handwash but bacterial levels are reduced after a proper hand wash for 30 seconds.arrow_forwardWhich of the following is NOT correctly matched? A. Halophile – microbe that can grow in an environment with high osmotic pressure (e.g., salt) B. None of the other four answers (all are correctly matched) C. Agar – complex polysaccharide from seaweed used as a solidifying agent in culture media D. Colony – a population of microbial cells arising from a single cell or spore or from a group of attached cells E. Capnophile – microbe requiring an elevated molecular oxygen (O2) environment for optimal growtharrow_forwardA. Antibiotics, such as gentamicin, are used in media to prevent growth of susceptible microbes. Is gentamicin media a selective, differential or enrichment media? B. Sorbitol is a sugar alcohol that all Escherichia bacteria can normally ferment into an acidic waste compound. This acidic end product is detected by including a pH indicator in the agar and noticing a color change. The strain of Escherichia that is a common food pathogen, E. coli O157:H7, however can not ferment sorbitol. It will grow similar to other E. coli, but not change the agar color. Is sorbitol in nutrient agar a selective, differential or enrichment media?arrow_forward
- Bacteria may be Gram + or Gram - due to their difference in staining. This stain is significant to treating bacterial diseases because A. Some people are immune to one type of bacteria but not the other B. The types of antibiotics prescribed will be based in part on which type of bacteria is causing the infection. C. Gram - always cause disease, Gram positives don't D. You can eliminate a large group of bacteria by knowing if infection is caused by either a Gram + or a Gram - which makes it easier to treat. E. B and D are correctarrow_forwardNote that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is one experiment you could do, involving culturing the organism?arrow_forwardNote that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is a procedure you could do, involving making a slide of the organism?arrow_forward
- Isolate #2: Cell Morphology (shape) . a. b. Isolate #2: Gram stain result Gram Postive. (The color I see on my screen is purple.) c. List at least two genera (not species) of bacteria that are consistent with the Gram reaction and morphology you observed. d. Are any of these genera are normal flora on the skin? If so which one(s)? (1 pt) ***Please answer the four questions base on the picture provided.***arrow_forwardComparing the different methods for counting bacterial cells, which of the following is FALSE? A. The serial dilution methods counts only viable cells. B. The turbidity method indirectly measures the number of cells observing how much light goes through a broth sample C. The turbidity method uses a spectrophotometer D. The direct microscopic method counts only viable cells E. The direct microscopic method counts both viable and non-viable cellsarrow_forwardThe colonies growing on the surface of solid media represent: Select one: a. A group of different bacteria b. A single bacterium c. A single cell d. Many cells of the same bacterium e. Can not tellarrow_forward
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