HUMAN BIOLOGY
16th Edition
ISBN: 9781260233032
Author: Mader
Publisher: RENT MCG
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Chapter 22.3, Problem 1CYP
Summary Introduction
To determine:
The polymerase chain reaction and DNA cloning to make gene copies.
Introduction:
The cloning or copy of the gene is required to determine the functions of the gene. The characteristic involves size, tissue distribution and expression of the gene. The cloning helps in the production of a high amount of protein.
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Outline the essential steps in the polymerase chain reaction.
Describe how a polymerase chain reaction is used to make copies of genes
Discuss how the polymerase chain reaction works.
Chapter 22 Solutions
HUMAN BIOLOGY
Ch. 22.1 - Describe the structure of a DNA molecule.Ch. 22.1 - Prob. 2LOCh. 22.1 - Prob. 3LOCh. 22.1 - Prob. 4LOCh. 22.1 - Prob. 1BTSCh. 22.1 - Prob. 2BTSCh. 22.1 - Prob. 1CYPCh. 22.1 - Prob. 2CYPCh. 22.1 - Prob. 3CYPCh. 22.1 - Prob. 4CYP
Ch. 22.2 - Prob. 1LOCh. 22.2 - Prob. 2LOCh. 22.2 - Prob. 3LOCh. 22.2 - Prob. 4LOCh. 22.2 - Prob. 5LOCh. 22.2 - Prob. 1CYPCh. 22.2 - Prob. 2CYPCh. 22.2 - Prob. 3CYPCh. 22.2 - Identify the various means of gene regulation in a...Ch. 22.3 - Prob. 1LOCh. 22.3 - Prob. 2LOCh. 22.3 - Understand the goals of genome editingCh. 22.3 - Prob. 4LOCh. 22.3 - Prob. 1BTHCh. 22.3 - If not everyone, do you think that convicted...Ch. 22.3 - Prob. 3BTHCh. 22.3 - Prob. 1BTSCh. 22.3 - What types of diseases might not be treatable...Ch. 22.3 - Prob. 1CYPCh. 22.3 - Prob. 2CYPCh. 22.3 - Summarize the reasons genetically modified...Ch. 22.4 - Prob. 1LOCh. 22.4 - Prob. 2LOCh. 22.4 - Prob. 3LOCh. 22.4 - Prob. 1BTSCh. 22.4 - Prob. 2BTSCh. 22.4 - Prob. 3BTSCh. 22.4 - Prob. 1CYPCh. 22.4 - Prob. 2CYPCh. 22.4 - Prob. 3CYPCh. 22 - Prob. 1ACh. 22 - Prob. 2ACh. 22 - 3. RNA is different from DNA in which of the...Ch. 22 - Prob. 4ACh. 22 - Prob. 5ACh. 22 - Prob. 6ACh. 22 - Prob. 7ACh. 22 - Prob. 8ACh. 22 - Prob. 9ACh. 22 - Prob. 10ACh. 22 - Prob. 11ACh. 22 - Prob. 12ACh. 22 - What are the advantages of using a recombinant DNA...Ch. 22 - Prob. 2TCCh. 22 - Prob. 3TCCh. 22 - Prob. 4TCCh. 22 - Prob. 5TC
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- Briefly describe the polymerase chain reaction. What is the difference between a step and a cycle?arrow_forwardExplain the role of reverse transcriptase in cloning.arrow_forwardWhat specifically will happen if DNA polymerase is inaccurate during DNA synthesis? Explain how this inaccuracy might affect the organism.arrow_forward
- With the use of well-illustrated diagrams, reconstruct the entire cloning process by explaining different stages of the cloning process that involves the following:a. Isolation of target DNA fragments (often referred to as inserts)b. Ligation of inserts into the plasmid, creating recombinant molecules c. Transformation of recombinant plasmids into bacteria or other suitable host for propagationd. Screening/selection of hosts containing the intended recombinant plasmid. For this stage(d), discuss the importance of a second marker that can be used for screening of genomic DNA for colonies containing the pka-1 under the principle of insertional inactivation. This should be properly explained using all the attributes of the plasmid described above.arrow_forwardOutline and discuss the steps in DNA cloningarrow_forwardExplain why PCR results in the amplification of a specific DNA sequence despite many competing sequencesarrow_forward
- Describe the purpose and process of DNA finger printing.arrow_forwardOutline the steps by which the polymerase chainreaction (PCR) amplifies a specific region of a genome.arrow_forwardExplain why the precise length of the template DNA sequence does not become amplified until the third cycle. Draw a picture that explains your answer. For: Electrophoresisarrow_forward
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