Whether there is a greater risk for E.coli O157:H7 infection when consuming a hamburger as compared to consuming a steak. Introduction: Food-borne illness or food-poisoning is a type of illness or infection that is caused by consumption of food contaminated with bacteria, virus, parasites and toxins. Bacteria, along with other toxin producing microbes contaminate the food items and once they are ingested, they can cause poisoning or infection which results in illness.

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Definition Definition Organism that lives in the body of another organism and obtains food at the expense of the host. A parasite can directly or indirect harm the host. There are two kinds of parasites: internal and external. An internal parasite lives inside another organism and is known as an endoparasite. An external parasite lives on the body surface of the host and is known as an ectoparasite. Protozoa and helminths are examples of major human parasites.

Chapter 22, Problem 9CTQ

(a)

Summary Introduction

To explain:

Whether there is a greater risk for E.coli O157:H7 infection when consuming a hamburger as compared to consuming a steak.

Introduction:

Food-borne illness or food-poisoning is a type of illness or infection that is caused by consumption of food contaminated with bacteria, virus, parasites and toxins. Bacteria, along with other toxin producing microbes contaminate the food items and once they are ingested, they can cause poisoning or infection which results in illness.

(b)

Summary Introduction

To discuss:

Whether or not food poisoning can still occur after consuming a reheated pot of soup known to be contaminated with Staphylococcus aureus.

Introduction:

Food-poisoning is a type of illness or infection is caused by consumption of food contaminated with bacteria, virus, parasites and toxins. The bacteria produce toxins in food items and once it is ingested it causes an infection which results in illness.

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Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…

Based on your results, which suspect's DNA best matches the DNA found at the crime scene?

Answer 2

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Definition Definition Organism that lives in the body of another organism and obtains food at the expense of the host. A parasite can directly or indirect harm the host. There are two kinds of parasites: internal and external. An internal parasite lives inside another organism and is known as an endoparasite. An external parasite lives on the body surface of the host and is known as an ectoparasite. Protozoa and helminths are examples of major human parasites.

Chapter 22, Problem 9CTQ

(a)

Summary Introduction

To explain:

Whether there is a greater risk for E.coli O157:H7 infection when consuming a hamburger as compared to consuming a steak.

Introduction:

Food-borne illness or food-poisoning is a type of illness or infection that is caused by consumption of food contaminated with bacteria, virus, parasites and toxins. Bacteria, along with other toxin producing microbes contaminate the food items and once they are ingested, they can cause poisoning or infection which results in illness.

(b)

Summary Introduction

To discuss:

Whether or not food poisoning can still occur after consuming a reheated pot of soup known to be contaminated with Staphylococcus aureus.

Introduction:

Food-poisoning is a type of illness or infection is caused by consumption of food contaminated with bacteria, virus, parasites and toxins. The bacteria produce toxins in food items and once it is ingested it causes an infection which results in illness.

Expert Solution & Answer

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Check out a sample textbook solution

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Answer 3

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Definition Definition Organism that lives in the body of another organism and obtains food at the expense of the host. A parasite can directly or indirect harm the host. There are two kinds of parasites: internal and external. An internal parasite lives inside another organism and is known as an endoparasite. An external parasite lives on the body surface of the host and is known as an ectoparasite. Protozoa and helminths are examples of major human parasites.

Chapter 22, Problem 9CTQ

(a)

Summary Introduction

To explain:

Whether there is a greater risk for E.coli O157:H7 infection when consuming a hamburger as compared to consuming a steak.

Introduction:

Food-borne illness or food-poisoning is a type of illness or infection that is caused by consumption of food contaminated with bacteria, virus, parasites and toxins. Bacteria, along with other toxin producing microbes contaminate the food items and once they are ingested, they can cause poisoning or infection which results in illness.

(b)

Summary Introduction

To discuss:

Whether or not food poisoning can still occur after consuming a reheated pot of soup known to be contaminated with Staphylococcus aureus.

Introduction:

Food-poisoning is a type of illness or infection is caused by consumption of food contaminated with bacteria, virus, parasites and toxins. The bacteria produce toxins in food items and once it is ingested it causes an infection which results in illness.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

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Answer 4

Question

Definition Definition Organism that lives in the body of another organism and obtains food at the expense of the host. A parasite can directly or indirect harm the host. There are two kinds of parasites: internal and external. An internal parasite lives inside another organism and is known as an endoparasite. An external parasite lives on the body surface of the host and is known as an ectoparasite. Protozoa and helminths are examples of major human parasites.

Chapter 22, Problem 9CTQ

(a)

Summary Introduction