Biochemistry: Concepts and Connections (2nd Edition)
Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 22, Problem 4P
Interpretation Introduction

(a) Interpretation:

A mixture of four α[P32] labelled rNTPs (ribonucleoside triphosphates) was added to permeabilized bacterial cells that are undergoing deoxyribonucleic acid (DNA) replication in the presence of an enzyme RNA polymerase inhibitor, and the incorporation into high-molecular-weight-material was followed over time (Shown in below graph). After 10 minutes of incubation, a 1000 fold excess of unlabeled rNTPs was added. The reason for adding excess of unlabeled rNTPs should be explained.

Concept Introduction:

Deoxyribonucleic acid (DNA) replication is the biological process through which two identical replicas of deoxyribonucleic acid (DNA) are synthesized from the one original deoxyribonucleic acid (DNA) molecule.

Pictorial representation:

The results of experiment are shown in the below:

Biochemistry: Concepts and Connections (2nd Edition), Chapter 22, Problem 4P

Interpretation Introduction

(b) Interpretation:

The reason of radioactivity being incorporated as ribonucleotides, instead of an alternative, like reduction to deoxyribonucleotides and the incorporation process should be explained.

Concept Introduction:

Deoxyribonucleic acid (DNA) replication is the biological process through which two identical replicas of deoxyribonucleic acid (DNA) are synthesized from the one original deoxyribonucleic acid (DNA) molecule.

Interpretation Introduction

(c) Interpretation:

The deoxyribonucleic acid (DNA) replication process in the experiment should be explained.

Concept Introduction:

Deoxyribonucleic acid (DNA) replication is the biological process through which two identical replicas of deoxyribonucleic acid (DNA) are synthesized from the one original deoxyribonucleic acid (DNA) molecule.

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DNA vs RNA (Updated); Author: Amoeba Sisters;https://www.youtube.com/watch?v=JQByjprj_mA;License: Standard youtube license