Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Chapter 21, Problem 1A
A laboratory test used to determine the identity of Staphylococcus aureus is its growth on mannitol salt agar. The medium contains 7.5% sodium chloride (NaCl). Why is it considered a selective medium for S. aureus?
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This is a catalase-positive, coagulase-negative, gram-positive coccus isolated from a urine specimen from a 20-year-old female college student. The image shown is a Mueller Hinton plate streaked with a 0.5 MacFarland standardized inoculum and a 5 microgram disk of novobiocin after overnight incubation. What is the identification of the isolate?
Please select the single best answer
Staphylococcus epidermidis
Staphylococcus aureus
Staphylococcus saprophyticus
Staphylococcus lugdenensis
Explain why the media where the Staphylococcus aureus is plated has turned yellow but the media where Staphyloccocus epidermidis plated is still pink? The media used would be the Mannitol Salt Agar Medium.
What is this organism? And what other test could be done to confirm it's identification?
1.Gram stain - positive cocci, chains
Catalase - weak positive
Hemolysis - beta
BE - positive
NaCl- positive
Bacitracin - no zone of inhibition
PYR - positive
Answer: Enterococcus spp. CAMP test to verify the identity
2.Gram positive cocci, chains
Catalase - negative
Hemolysis - alpha
BE - negative
NaCl - negative
P disk - resistant
Bile Solubility - negative
Answer - viridans group, Use PYR test to verify
Chapter 21 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 21 - Discuss the usual mode of entry of bacteria into...Ch. 21 - What bacteria are identified by a positive...Ch. 21 - Prob. 3RCh. 21 - Complete the table of epidemiology below.Ch. 21 - Why do some states require a test for antibodies...Ch. 21 - Prob. 6RCh. 21 - Prob. 7RCh. 21 - Prob. 8RCh. 21 - Prob. 9RCh. 21 - Prob. 10R
Ch. 21 - Prob. 1MCQCh. 21 - Prob. 2MCQCh. 21 - Prob. 3MCQCh. 21 - A 12-year-old boy had a fever, rash, headaches,...Ch. 21 - A patient has conjunctivitis. If you isolated...Ch. 21 - You microscopically examine scrapings from a case...Ch. 21 - Prob. 7MCQCh. 21 - Prob. 8MCQCh. 21 - Prob. 9MCQCh. 21 - Prob. 10MCQCh. 21 - A laboratory test used to determine the identity...Ch. 21 - Prob. 2ACh. 21 - Prob. 3ACh. 21 - Prob. 4ACh. 21 - Prob. 1CAECh. 21 - Prob. 2CAECh. 21 - Prob. 3CAE
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- Describe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like (color) and explain does it grow on that agar. Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutient Agararrow_forwardYou are interested in obtaining Staphylococcus aureus for a study investigating the prevalence of methicillin-resistant Staphylococcus aureus in the general population. You have received several samples and are ready to start your isolation procedures ,State if you use general or selective media and which specific media you would choose. How would you determine if the culture was contaminatedarrow_forwardNot only is blood agar an enriched medium that can support fastidious organism growth, but it can also serve as a differential medium in the identification of streptococcal species because of the presence of red blood cells that can be destroyed by an organism's hemolysins. Looking at this photo, how could you describe the organism growing on the surface of this blood agar plate? Multiple Choice a) Gamma-hemolytic b) Alpha-hemolytic c) Non-hemolytic d) Beta-hemolyticarrow_forward
- Describe the appearance of an S. agalactiae colony grown on blood agar. Describe how that colony would differ in appearance from a colony of S. pyogenes.arrow_forwardBased on the journal article “Interpretation of the Tube Coagulase Test for Identification of Staphylococcus aureus”, what should be the result to conclude the organism is S.aureus? When is it necessary to have supplemental tests done for the identification of S.aureus?arrow_forwardYou are working in a lab studying Streptococcus pyogenes as a cause of necrotizing fasciitiis. You have an overnight culture that you want to know the starting concentration of, so do a set of six 1:10 serial dilutions (putting 1 mL from the stock into a 9 mL blank), with tube #1 being 1:10, #2 is 1:100, etc. You plate 0.1 mL from tube 5 onto a blood agar plate and the next morning count 134 colonies. How many bacteria (measured in CFU/mL) were in the overnight culture flask? A. 1.34 x 10^4 CFU/mL B. 1.34 x 10^5 CFU/mL C. 1.34 x 10^6 CFU/mL D. 1.34 x 10^7 CFU/mL E. 1.34 x 10^8 CFU/mL F. cannot tell based on the data given - you'd need to know the volume of the original culture flaskarrow_forward
- You mixed up the numbers on the tubes when you inoculated the mannitol salt agar (MSA) plate. You do not know if you grew staph epidermis or E. coli. You found that the organism growing on the mannitol salt agar remained red after incubation. It is most likely that the organism is E.coli. a) True b) Falsearrow_forwardWhy is glucose not the choice of carbohydrate for most differential media used in the identification of gram (-) enteric bacilli?arrow_forwardwhat treatment can kill the positive result of staphylococcus aureus in a catalase method ?arrow_forward
- Do you think Rickettsia rickettsii can grow on blood agar? Explain.arrow_forwardWhat is the result of Staphylococcus epidermidis in the TSI agar test?arrow_forwardI AM TRYING TO IDENTIFY THIS UNKNOWN. IMAGE 1 HAS TWO PICTURE OF CATALASE TEST AND BLOOD AGAR TEST. I believe it is one of the following: 1) S. pyo. 2)S. agal . 3)S.pneu. 4)E. faecalis 5)S. aureus 6)S epi. 7)S. sapro. 8)M. luteus Please let me know which test will i need out of the table to justify your reason of picking up the unknown and also how that test justifies it? what characteristics of that test made you pick the unknown?arrow_forward
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