Chapter 20, Problem 93IAE

Interpretation Introduction

Interpretation:

The expression for $K_{\text{M}}$ and $k_2$ should be determined.

Concept introduction:

Michaelis-Menten equation explains kinetics of an enzyme behavior that acts according to simple model.

The simple model of kinetics of an enzyme behavior can be represented as follows:

$\text{E}+\text{S}\underset{k_{-1}}{\overset{k_1}{\rightleftharpoons }}\text{ES}\stackrel{k_2}{\to }\text{E}+\text{P}$

The Michaelis-Menten equation for this system is,

$V=\frac{V_{\text{max}}\left[\text{S}\right]}{K_{\text{M}}+\left[\text{S}\right]}$