Prescott's Microbiology
Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 19, Problem 1CHI

Consider the fact that the use of 16S rRNA sequencing as a taxonomic and phylogenetic tool has resulted in tripling the number of bacterial phyla. Why has the advent of this genetic technique expanded the currently accepted number of microbial phyla?

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Summary Introduction

The taxonomy is the study of classification and naming the organism based on their shared characteristic features. Phylogeny is the study of evolutionary changes occurs in an organism or group of organism. Gene sequencing is a genetic tool or phylogenetic tool that expands the number of microbial phyla.

Explanation of Solution

Prokaryotes have 70S ribosome which is divided into 30S and 40S subunits. 30S subunit consists of 16S ribosomal RNA (16S rRNA). The gene that codes for 16S rRNA is called 16S rRNA gene and the nucleotide sequencing of this gene is called 16S rRNA sequencing. It is used in reconstructing microbial phyla since the rate of evolution at this region is very slow. This tool allows the discovery of many new organisms.

Earlier to this genetic tool classification was made on the basis of morphological, biochemical, and physiological characteristics of the organism. Distinguishing phyla based on these characteristic features do not give much resolution as 16S rRNA.

16S rRNA sequencing tool overcomes some problems with conventional classification method. One such problem is culturing the microbes in the lab. Some microbes cannot be cultured or very difficult to culture in a lab. These microbes also can be identified using 16S rRNA sequencing.

Therefore, the advent of this genetic technique currently expanded the number of microbial phyla. 

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Let's say that you're a scientist trying to analyze newly sequenced bacterial DNA genomes from an environmental sample of rainforest soil, and your first task is to organize all of the genomes you find into a phylogenetic tree. If you couldn't use the 16S rRNA gene sequence to create the tree, which of these alternative genes would work best for your task of creating a tree that works for all the bacteria in your sample? The gene for Bacteriochlorophyll A A gene for an RNA polymerase component protein The first gene in the histidine biosynthesis pathway The first gene in the LPS synthesis pathway
select the correct options: A) For organisms that diverged  >74 mya, ignore 3rd base positions within codons because they will offer little phylogenetic information for organisms. B) use different parts ofthe genome to estimate different parts of a phylogeny C) Use only 1st base positions within codons because they increase rapidly at first, then level off as result of single subsitituions at the same sites D) Use slowly evolvong sites in the squecne, such as 2nd base positions within codonsfor analyzing the oldest relationships among organisms.
Give typing answer with explanation and conclusion  In thinking about the clinical application of phylogenetics, discuss the role of phylogenetics in these areas.   a) Predictions of drug resistance   b) Identifying the origin of an outbreak   c)Predicting the spread of a disease

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Prescott's Microbiology

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Bacterial Genomics and Metagenomics; Author: Quadram Institute;https://www.youtube.com/watch?v=_6IdVTAFXoU;License: Standard youtube license