Foundations in Microbiology
10th Edition
ISBN: 9781259705212
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Chapter 17.L1, Problem 6WC
Summary Introduction
Introduction:
Unlike other organisms, viruses are difficult to culture and identify and re9quire special techniques for their diagnosis. Their identification can be done using genetic techniques.
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How does PCR help in the identification of the virus from patients?
After giving brief information about the viruses, discuss briefly what is meant by their virulent effects on their hosts and in which two ways this effect occurs. Comment and illustrate the use of viruses within the scope of Recombinant DNA Technology.
Discuss the principles behind the use of the following techniques in the diagnosis of viral infections, providing examples of viruses diagnosed using these methods
5..1 ELISA
5.2 Restriction Fragment Length Polymorphism
5.3 Polymerase chain reaction
Chapter 17 Solutions
Foundations in Microbiology
Ch. 17.1 - Describe what is involved in the main categories...Ch. 17.1 - Explain several techniques in specimen collection...Ch. 17.1 - Summarize the main procedures in isolation,...Ch. 17.1 - Summarize the major techniques in identifying and...Ch. 17.1 - Describe the general principles in specimen...Ch. 17.1 - Explain why it is important to prevent microbes...Ch. 17.1 - Summarize the kinds of tests that are used to...Ch. 17.2 - Describe some direct methods of testing a...Ch. 17.2 - Summarize the aims in selection of culture...Ch. 17.2 - Prob. 6ELO
Ch. 17.2 - Describe how flowcharts and comparison tables are...Ch. 17.3 - Explain the different variations on genetic...Ch. 17.3 - Describe what is involved in direct specimen...Ch. 17.3 - Prob. 6CYPCh. 17.3 - Describe the applications of PCR in identification...Ch. 17.4 - Describe the background aims of immunologic...Ch. 17.4 - Identify how antigen-antibody reactions are...Ch. 17.4 - Prob. 11ELOCh. 17.4 - Explain the basic methods behind the Western blot...Ch. 17.4 - Prob. 13ELOCh. 17.4 - What is the basis of serology and serological...Ch. 17.4 - Differentiate between specificity and sensitivity.Ch. 17.4 - Prob. 10CYPCh. 17.4 - Prob. 11CYPCh. 17.4 - Prob. 12CYPCh. 17.4 - Prob. 13CYPCh. 17.4 - Give examples of several tests that employ...Ch. 17.4 - What is meant by complement fixation? What are...Ch. 17.4 - Prob. 16CYPCh. 17.5 - Describe the concepts behind the main types of...Ch. 17.5 - Prob. 15ELOCh. 17.6 - Prob. 16ELOCh. 17.6 - Prob. 17CYPCh. 17.6 - Prob. 18CYPCh. 17.6 - Prob. 19CYPCh. 17.6 - Prob. 20CYPCh. 17.6 - Observing figure 17.17, indicate whether each...Ch. 17.L1 - Multiple Matching. Match each of the following...Ch. 17.L1 - Prob. 2MCQCh. 17.L1 - Prob. 3MCQCh. 17.L1 - Prob. 4MCQCh. 17.L1 - A patient with a _____ titer of antibodies to an...Ch. 17.L1 - Prob. 6MCQCh. 17.L1 - Prob. 7MCQCh. 17.L1 - An example of an in vivo serological test is a....Ch. 17.L1 - Which of the following specimens must be removed...Ch. 17.L1 - Prob. 1CSRCh. 17.L1 - Prob. 2CSRCh. 17.L1 - Prob. 3CSRCh. 17.L1 - Prob. 1WCCh. 17.L1 - Prob. 2WCCh. 17.L1 - Briefly describe the principles and give an...Ch. 17.L1 - Prob. 4WCCh. 17.L1 - Prob. 5WCCh. 17.L1 - Prob. 6WCCh. 17.L2 - Prob. 1CTCh. 17.L2 - Prob. 2CTCh. 17.L2 - Why do some tests for antibody in serum (such as...Ch. 17.L2 - Prob. 4CTCh. 17.L2 - Prob. 5CTCh. 17.L2 - Prob. 6CTCh. 17.L2 - From chapter 3, fig 3.17a (reproduced on the...Ch. 17.L2 - Prob. 2VC
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- Explain how PCR/OLA (polymerase chain reaction/oligonucleotide ligation assay) can be used in the diagnosis of sickle cell disorder . Would you recommend this method for routine diagnosis of sickle cell disorder? Explainarrow_forwardDiscuss the principles behind the use of the following techniques in the diagnosis of viral infections, providing examples of viruses diagnosed using these methods: Restriction Fragment Length Polymorphismarrow_forwardDescribe how we can use genetic techniques to identify the presence of viral genetic information in a patient sample.arrow_forward
- describe how the bacteria should have the ability to be recovered back from the infected host for the purpose of experimentation including sequencing ,vitro culturing and polymerase chain reaction amplification.arrow_forwardPlease introduce how to obtain a nucleotide sequence of unknown viruses. Please give me a detailed process.arrow_forwardWhat technique must be used to visualize a virus?arrow_forward
- Describe several uses of genetically modified bacteria and virusesin medicine or industry.arrow_forwardHow do you use CRISPR to treat a patient with sickle cell anemia? Please provide a detailed explanationarrow_forwardWhat technique could be used to confirm the presence or absence of plasmids in the donor, recipient and transconjugant strains?arrow_forward
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