Foundations in Microbiology
10th Edition
ISBN: 9781259705212
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
expand_more
expand_more
format_list_bulleted
Question
Chapter 17.4, Problem 10CYP
Summary Introduction
Introduction:
There are several ways in which antigen-antibody (Ag-Ab) reactions can be detected. They can be detected either by naked eyes or microscopically.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
The authors have used a chiral HPLC column (separating phase) to analyze compounds 4 and 5. Briefly describe a) the way a chiral HPLC column works to separate natural products of the Actinopyrone family and b) briefly comment on the general applicability of chiral HPLC columns in natural products research.
In Experiment 7, Isolation of Citrus Oils, the compounds isolated were the monoterpene compounds alpha-pinene, beta-pinene,
limonene, myrcene and gamma-terpinene. The citrus oils were analyzed using gas chromatography, which separates compounds
based on polarity and boiling point. Column chromatography and thin layer chromatography work on similar separation principles
as gas chromatography, but rely only on polarity of compounds and solvents for separation. Explain why neither column
chromatography nor thin layer chromatography would be appropriate separation methods for the terpenes isolated in Experiment
7.
Answer:
Compounds I, II, and III are in the following biochemical pathway:
precursor
compound I
enzyme
A
enzyme
B
compound II
► compound III
enzyme
Mutation a inactivates enzyme A, mutation b inactivates enzyme B, and
mutation c inactivates enzyme C. Mutants, each having one of these
defects, were tested on minimal medium to which compound I, II, or III
was added. Fill in the results expected of these tests by placing a plus
sign (+) for growth or a minus sign (-) for no growth in the table below.
Minimal medium to which is added
Strain with mutation Compound I Compound II Compound III
Chapter 17 Solutions
Foundations in Microbiology
Ch. 17.1 - Describe what is involved in the main categories...Ch. 17.1 - Explain several techniques in specimen collection...Ch. 17.1 - Summarize the main procedures in isolation,...Ch. 17.1 - Summarize the major techniques in identifying and...Ch. 17.1 - Describe the general principles in specimen...Ch. 17.1 - Explain why it is important to prevent microbes...Ch. 17.1 - Summarize the kinds of tests that are used to...Ch. 17.2 - Describe some direct methods of testing a...Ch. 17.2 - Summarize the aims in selection of culture...Ch. 17.2 - Prob. 6ELO
Ch. 17.2 - Describe how flowcharts and comparison tables are...Ch. 17.3 - Explain the different variations on genetic...Ch. 17.3 - Describe what is involved in direct specimen...Ch. 17.3 - Prob. 6CYPCh. 17.3 - Describe the applications of PCR in identification...Ch. 17.4 - Describe the background aims of immunologic...Ch. 17.4 - Identify how antigen-antibody reactions are...Ch. 17.4 - Prob. 11ELOCh. 17.4 - Explain the basic methods behind the Western blot...Ch. 17.4 - Prob. 13ELOCh. 17.4 - What is the basis of serology and serological...Ch. 17.4 - Differentiate between specificity and sensitivity.Ch. 17.4 - Prob. 10CYPCh. 17.4 - Prob. 11CYPCh. 17.4 - Prob. 12CYPCh. 17.4 - Prob. 13CYPCh. 17.4 - Give examples of several tests that employ...Ch. 17.4 - What is meant by complement fixation? What are...Ch. 17.4 - Prob. 16CYPCh. 17.5 - Describe the concepts behind the main types of...Ch. 17.5 - Prob. 15ELOCh. 17.6 - Prob. 16ELOCh. 17.6 - Prob. 17CYPCh. 17.6 - Prob. 18CYPCh. 17.6 - Prob. 19CYPCh. 17.6 - Prob. 20CYPCh. 17.6 - Observing figure 17.17, indicate whether each...Ch. 17.L1 - Multiple Matching. Match each of the following...Ch. 17.L1 - Prob. 2MCQCh. 17.L1 - Prob. 3MCQCh. 17.L1 - Prob. 4MCQCh. 17.L1 - A patient with a _____ titer of antibodies to an...Ch. 17.L1 - Prob. 6MCQCh. 17.L1 - Prob. 7MCQCh. 17.L1 - An example of an in vivo serological test is a....Ch. 17.L1 - Which of the following specimens must be removed...Ch. 17.L1 - Prob. 1CSRCh. 17.L1 - Prob. 2CSRCh. 17.L1 - Prob. 3CSRCh. 17.L1 - Prob. 1WCCh. 17.L1 - Prob. 2WCCh. 17.L1 - Briefly describe the principles and give an...Ch. 17.L1 - Prob. 4WCCh. 17.L1 - Prob. 5WCCh. 17.L1 - Prob. 6WCCh. 17.L2 - Prob. 1CTCh. 17.L2 - Prob. 2CTCh. 17.L2 - Why do some tests for antibody in serum (such as...Ch. 17.L2 - Prob. 4CTCh. 17.L2 - Prob. 5CTCh. 17.L2 - Prob. 6CTCh. 17.L2 - From chapter 3, fig 3.17a (reproduced on the...Ch. 17.L2 - Prob. 2VC
Knowledge Booster
Similar questions
- For the same chromatographic column and experimental conditions Rs when H = 3 cm = Rs when H = 5 cm Rs when H = 3 cm > Rs when H = 5 cm Rs when H = 3 cm < Rs when H = 5 cm The two quantities cannot be compared due to insufficient information.arrow_forwardThe Cavity Ring-Down Spectroscopy (CRDS) technique offers greater sensitivity compared to other conventional spectroscopy techniques. Explain the underlying mechanism of this.arrow_forwardDescribe various detection methods used in High-Performance Liquid Chromatography (HPLC) and types of biomolecules that are detected in each method?arrow_forward
- What is the role of imidazole in Ni-Affinity Chromatography?arrow_forwardA vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show workingarrow_forwardState the principle that underlies the following biochemical tests: a. Voges-Proskauer testarrow_forward
- Define the following terms:a. alkylating agentb. base analoguec. nonalkylating agentd. intercalating agente. ethidium bromidearrow_forwardWhich among the general tests for alkaloids can be used as a visualizing agent in detecting alkaloids at TLC chromatogram? Explain.arrow_forwardState the principle that underlies the following biochemical tests: a) Methyl red testarrow_forward
- What is known as montreal protocol?arrow_forwardState the principle that underlies the following biochemical tests: a) Methyl red test b) Voges-Proskauer testarrow_forwardSuccinic acid is produced by Actinobacillus succinogenes with the following medium composition (g/L): glucose, 50; yeast extract, 5; NaCl, l; Na2HPO4, 0.3; NaH2PO4, 1.4; K2HPO4, 1.5; MgC12.6H20, 0.2; CaC12.2H20, 0.23. The pH is controlled and maintained at 3.63. Estimate the range of upper limit, if 0.8% of deadband is applied. With a schematic diagram, explain how to maintain the pH throughout the fermentation process.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Principles Of Radiographic Imaging: An Art And A ...Health & NutritionISBN:9781337711067Author:Richard R. Carlton, Arlene M. Adler, Vesna BalacPublisher:Cengage Learning
Principles Of Radiographic Imaging: An Art And A ...
Health & Nutrition
ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Cengage Learning