Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 16.1, Problem 1RIA
Retrieve, Infer, Apply
List three ways in which spontaneous mutations might arise.
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Chapter 16 Solutions
Prescott's Microbiology
Ch. 16.1 - Retrieve, Infer, Apply List three ways in which...Ch. 16.1 - Compare and contrast the means by which the...Ch. 16.1 - Give examples of intragenic and extragenic...Ch. 16.1 - Retrieve, Infer, Apply Sometimes a point mutation...Ch. 16.1 - Retrieve, Infer, Apply Why might a missense...Ch. 16.2 - How would you screen for a tryptophan auxotroph?...Ch. 16.2 - Why is a small amount of histidine added to the...Ch. 16.2 - Retrieve, Infer, Apply Describe how replica...Ch. 16.2 - Retrieve, Infer, Apply Why are mutant selection...Ch. 16.2 - Retrieve, Infer, Apply Briefly discuss how...
Ch. 16.2 - Design an experiment that selects for mutants of a...Ch. 16.2 - Describe how you would isolate a mutant that...Ch. 16.2 - Prob. 6RIACh. 16.3 - How is mismatch repair similar to DNA polymerase...Ch. 16.3 - How is damaged DNA recognized by the UvrAB...Ch. 16.3 - Prob. 1RIACh. 16.3 - Retrieve, Infer, Apply What role does DNA...Ch. 16.3 - Retrieve, Infer, Apply When E. coli cells are...Ch. 16.3 - Explain how the following DNA alterations and...Ch. 16.4 - An antibiotic-resistance gene located on a...Ch. 16.4 - What are the three mechanisms of horizontal gene...Ch. 16.4 - What four fates can DNA have after entering a...Ch. 16.4 - How does homologous recombination differ from...Ch. 16.5 - What features are common to all types of...Ch. 16.5 - How does a transposon differ from an insertion...Ch. 16.5 - What is simple (cut-and-paste) transposition? What...Ch. 16.5 - What effect would you expect the existence of...Ch. 16.6 - Prob. 1MICh. 16.6 - What is bacterial conjugation and how was it...Ch. 16.6 - For F+, Hfr, and F strains of E. coli, indicate...Ch. 16.6 - Describe how F+ F and Hfr conjugation processes...Ch. 16.6 - Compare and contract F+ F and F F conjugation.Ch. 16.7 - According to this model, what would happen if DNA...Ch. 16.7 - Define transformation and competence.Ch. 16.7 - Describe how transformation occurs in S....Ch. 16.7 - Discuss two ways in which artificial...Ch. 16.8 - What is the term used to describe a temperate...Ch. 16.8 - Compare the number of transducing particles that...Ch. 16.8 - Describe generalized transduction and how it...Ch. 16.8 - What is specialized transduction and how does it...Ch. 16.8 - How might one tell whether horizontal gene...Ch. 16.8 - Why doesnt a cell lyse after successful...Ch. 16.8 - Describe how conjugation, transformation, and...Ch. 16.9 - Why cant the gal and bio genes be transduced by...Ch. 16.9 - As a replicative transposon, what would happen if...Ch. 16 - Mutations are often considered harmful. Give an...Ch. 16 - Mistakes made during transcription affect the cell...Ch. 16 - Suppose that transduction took place when a U-tube...Ch. 16 - Suppose that you carried out a U-tube experiment...Ch. 16 - Prob. 5CHICh. 16 - Studies of phage therapy to treat bacterial...Ch. 16 - Enterococcus faecalis is a major cause of...
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- Amplified target regions of four different samples were separated using gel electrophoresis. DNA fragments labeled with the isotope P32 were separated by gel electrophoresis. P32 was used to a) label fragments for imaging. b) distinguish between the fragment’s 3’ and 5’. c) observe the location of the fragments. d)speed up the rate of separation by electrophoresisarrow_forwardQUESTION: what difficulty will you experience if you do genetic manipulation to streptomyces spp. and how can this difficulty overcome ?? how could you modulate the gene expression for improving the productivity of an antibiotic produced by the streptomyces strain ? discuss with diagramarrow_forwardDiscuss the underlying biochemical principle of the nucleic acid sequencing methods known as Semiconductor (Ion Torrent) sequencing.arrow_forward
- f) Examine the advantage of DNA based biosensor as compared to enzyme biosensor?arrow_forwardWith detail, compare and contrast the following 5 real-time assays; Taqman, SYBR Green, Molecular Beacon, FRET, and Scorpion. Please write complete sentences in paragraph form. Identify how the assays are similar but more importantly, what are the distinguishing features of each of the technologies.arrow_forwardPlease answer fast We have lots of options when it comes to trying to purposefully make mutations while doing research. Please brieflyname/describe a method you could use to make: a) random mutations anywhere within the genome in living cells (in vivo) b) random mutations within our cloned gene of interest in vitro c) a very specific (non-random) mutation within our cloned gene of interest in vitro d) NOW for the answer you gave in part C, please elaborate in detail how this method of mutagenesis is performed.arrow_forward
- Provide detailed explanation of the following super-resolution techniques: GSD, RESOLFT, SPDM, PAINT or DNA-PAINT.arrow_forwardFrom where Probes are derived ?arrow_forwardDiscuss how DNA extraction of microbial DNA directly can be done from infected tissue through an optimized protocol for use in nanopore sequencing.arrow_forward
- Who revealed biochemical nature of the transforming principle?how was it done?arrow_forwardexplain the method of Identifying mutant genes by plasmid librarytransformationarrow_forwardElaborate on three (3) lysis methods used to disrupt bacterial cells. During DNA isolation using P: C: I technique, a precipitation step is required. What is the purpose of this step? How can sodium acetate and ethanol facilitate the precipitation step in DNA isolation?arrow_forward
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