Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 16, Problem 2CHI
Mistakes made during transcription affect the cell but not considered “mutations.” Why not?
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Listed below are steps in the transcription process. Reorganize the list so the steps in the correct order- starting with the first step in initiating transcription and ending with completion of a new strand of RNA (in other words- from start to finish of transcription).
RNA polymerase reaches the termination signal
DNA unwinds underneath RNA polymerase at transcription start site
RNA polymerase is recruited to the promoter region
mRNA transcript is released
General Transcription factors bind TATA box (and other DNA sequences) in the promoter region
General Transcription Factors unbind from promoter region
mRNA transcript synthesis occurs
RNA polymerase moves along the template strand in the 3’ to 5’ direction
Hydrogen bonds are important in DNA replication and transcription. They are relatively weak chemical bonds. Why is this a desirable feature for DNA?
Describe the effect (s) of changing (mutating) the promoter on the transcription of the DNA strand/gene the promoter controls.
What happens to protein synthesis if a nonsense codon is inserted into the gene?
Explain why a point mutation does not necessarily change the original amino acid sequence. (Explain silent mutations)
Choose any pentapeptide composed of five different amino acids. List the amino acids. Present one messenger RNA codon for each amino acids and the sequence of nucleotides on the DNA that originally coded for your pentapeptide.
Matching type
Choices are in the picture
1. simultaneous and rapid process producing mRNA and polypeptide
2. cleaving the polypeptide by adding water
3. three initiation factors are required to commence the process
4. removal of gene segment disrupting the message
5. single mRNA codes for the proteome
Chapter 16 Solutions
Prescott's Microbiology
Ch. 16.1 - Retrieve, Infer, Apply List three ways in which...Ch. 16.1 - Compare and contrast the means by which the...Ch. 16.1 - Give examples of intragenic and extragenic...Ch. 16.1 - Retrieve, Infer, Apply Sometimes a point mutation...Ch. 16.1 - Retrieve, Infer, Apply Why might a missense...Ch. 16.2 - How would you screen for a tryptophan auxotroph?...Ch. 16.2 - Why is a small amount of histidine added to the...Ch. 16.2 - Retrieve, Infer, Apply Describe how replica...Ch. 16.2 - Retrieve, Infer, Apply Why are mutant selection...Ch. 16.2 - Retrieve, Infer, Apply Briefly discuss how...
Ch. 16.2 - Design an experiment that selects for mutants of a...Ch. 16.2 - Describe how you would isolate a mutant that...Ch. 16.2 - Prob. 6RIACh. 16.3 - How is mismatch repair similar to DNA polymerase...Ch. 16.3 - How is damaged DNA recognized by the UvrAB...Ch. 16.3 - Prob. 1RIACh. 16.3 - Retrieve, Infer, Apply What role does DNA...Ch. 16.3 - Retrieve, Infer, Apply When E. coli cells are...Ch. 16.3 - Explain how the following DNA alterations and...Ch. 16.4 - An antibiotic-resistance gene located on a...Ch. 16.4 - What are the three mechanisms of horizontal gene...Ch. 16.4 - What four fates can DNA have after entering a...Ch. 16.4 - How does homologous recombination differ from...Ch. 16.5 - What features are common to all types of...Ch. 16.5 - How does a transposon differ from an insertion...Ch. 16.5 - What is simple (cut-and-paste) transposition? What...Ch. 16.5 - What effect would you expect the existence of...Ch. 16.6 - Prob. 1MICh. 16.6 - What is bacterial conjugation and how was it...Ch. 16.6 - For F+, Hfr, and F strains of E. coli, indicate...Ch. 16.6 - Describe how F+ F and Hfr conjugation processes...Ch. 16.6 - Compare and contract F+ F and F F conjugation.Ch. 16.7 - According to this model, what would happen if DNA...Ch. 16.7 - Define transformation and competence.Ch. 16.7 - Describe how transformation occurs in S....Ch. 16.7 - Discuss two ways in which artificial...Ch. 16.8 - What is the term used to describe a temperate...Ch. 16.8 - Compare the number of transducing particles that...Ch. 16.8 - Describe generalized transduction and how it...Ch. 16.8 - What is specialized transduction and how does it...Ch. 16.8 - How might one tell whether horizontal gene...Ch. 16.8 - Why doesnt a cell lyse after successful...Ch. 16.8 - Describe how conjugation, transformation, and...Ch. 16.9 - Why cant the gal and bio genes be transduced by...Ch. 16.9 - As a replicative transposon, what would happen if...Ch. 16 - Mutations are often considered harmful. Give an...Ch. 16 - Mistakes made during transcription affect the cell...Ch. 16 - Suppose that transduction took place when a U-tube...Ch. 16 - Suppose that you carried out a U-tube experiment...Ch. 16 - Prob. 5CHICh. 16 - Studies of phage therapy to treat bacterial...Ch. 16 - Enterococcus faecalis is a major cause of...
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- A cell has a mutated mediator protein. a) How will this affect transcription of genes? (circle an answer below) No transcription Weak transcription will occur Strong transcription will occur b) Why? Describe your choice abovearrow_forwardWhy will a mistake in the RNA code alone not become a mutation?arrow_forwardFor each mutant, state what change has occurred in the DNA, whether it was a substitution by transition or transversion, sense mutation, nonsense or reading frame change. It must present the codon sequence. Normal nucleotide sequence starting from the third codon: CCC-ACG-GUG-ACG-ACA-CGG-UGG Please show the codon and nucleotide sequence of the mutation.arrow_forward
- Consider this list (below) of steps involved in transcription. These steps are out of order. TRANSCRIPTION: 1. mRNA travels through a nuclear pore and enters the cytoplasm 2. the mRNA polymerase attaches at the start of a specific gene 3. RNA polymerase reads the gene surface4. a transcription factor bonds to a promoter site5. DNA molecule is unwound 6. a complimentary mRNA is produced What is the correct order of this transcription?arrow_forwardWhich of the following statements regarding complementary molecules in transcription is false? Group of answer choices The sense strand is complementary to the template strand The transcribed RNA is complementary to the noncoding strand The coding strand is complementary to the antisense strand The transcribed RNA is complementary to the coding strandarrow_forwardShown below is an eukaryotic gene. Assuming normal wild type RNA processing in a.cell, which of the following mature MRNAS could result in normal levels of functional synthesized proteins? Select all that apply Direction of transcription Promoter Template strand 5' Exon 4 Intron 3 Exon 3 Intron 2 Exon 2 Intron 1 Exon 1 3' 5' Coding strand Transcription start Transcription start 5' CAP-Exon1-Exon3-Exon4-AA..AAAA 5' CAP-Exon1-Exon2-Exon3-Exon4-AA...AAAA 5' CAP-Exon1-Exon2-Exon3-Exon4 Exon1-Exon2-Exon3-Exon4-.....AAAAarrow_forward
- Question 10 Review mutations. Match the term and its description. Each term can only be used once. changes in just one nucleotide pair of a gene | Choose | These mutations have no effect on the amino acid produced by a codon because of redundancy in the genetic code [ Choose J These mutations still code for an amino acid, but not the correct | Choose | amino acid These mutations change an amino acid codon into a stop codon: most lead to a nonfunctional protein | Choose In this mutation, insertion or deletion of nucleotides may alter the reading frame | Choose | additions of nucleotide pairs in a gene | Choose > > > > >arrow_forwardWhich of the following components is involved in the initiation of transcription? Group of answer choices promoter primer start codon originarrow_forwardName of the enzyme that adds RNA Nucleotides during Transcription? O Helicase O Primase DNA Polymerase O RNA Polymerase 9MDarrow_forward
- Insert Table Chart Text Shape Media Comment Which of the following statements regarding "start" and "stop" signals apply to both transcription and translation? the start and stop signals are "read" (i.e., interpreted) the start and stop signals reside within nucleic acid the start and stop signals are part of the genetic code the start and stop signals reside in RNA. start and stop signals are needed because the products must have a definite length and sequence.arrow_forwardI’m having trouble choosing which mRNA transcripts would not be translated?arrow_forwardInitiation of transcription is when O a MRNA transcript is synthesized. the polymerase enzyme dissociates from the DNA. the polymerase enzyme binds to the promoter. none of the choices are correct.arrow_forward
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