Biology
Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 16, Problem 2CQ
Summary Introduction

To identify: The copies of the OCA2 gene that influences eye color in humans.

Introduction: A karyotype helps in the identification of number, form, and size of chromosomes present within an actively dividing cell. Scientists discovered that eukaryotic chromosomes occur in sets by studying the karyotype of various species. The cells are treated with drugs before studying or making a karyotype.

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Below are two pictures of perithecium on microscope slides that have been squished under cover slips so that the asci inside have spread out. In the asci, we can clearly see the tan ascospores (light colored) and wildtype ascospores (dark colored). We will perform our tetrad analysis by counting how many of the asci are non-recombinant and how many of the acsi are recombinant. We can tell which are recombinant and non-recombinant by observing the patterns of the ascospores in the asci. See the patterns of ascospores for non- recombinant and recombinant asci in the table below. Ascospore pattern of non-recombinant asci OOOO OOOOO You will count the total number of non-recombinant and recombinant asci in the two pictures below. You will only count the asci that have a star next to them because these asci can be clearly identified as non-recombinant or recombinant. Select the correct number below after you are done. Total number of non-recombinant asci [Select] Ascospore pattern of…
A blood stain from a crime scene and blood samples from four suspects were analyzed by PCR using fluorescent primers associated with three STR loci: D3S1358, vWA, and FGA. The resulting electrophoretograms are shown below. The numbers beneath each peak identify the allele (upper box) and the height of the peak in relative fluorescence units (lower box). Solve, (a) Since everyone has two copies of each chromosome and therefore, two alleles of each gene, what accounts for the appearance ofonly one allele at some loci? (b) Which suspect is a possible source of the blood? (c) Could the suspect be identifi ed using just one of the three STR loci? (d) What can you conclude about the amount of DNA obtained from Suspect 1 compared to Suspect 4?
Now that you have counted the non-recombinant and recombinant asci we want to determine the recombination frequency between the tan gene and the centromere. To calculate the recombination frequency we need an accurate count of the number of non-recombinant recombinant individuals. In the asci the individuals are the ascospores. and 1118 WWW 4 non- recombinant ascospores 4 non- recombinant ascospores 1838 W M M M 500 2 non- recombinant ascospores 2 recombinant ascospores 2 recombinant ascospores 2 non- recombinant ascospores Figure 1.6. The number of ascospores in non-recombinant and recombinant asci. In the non-recombinant asci all 8 ascospores are non-recombinant (see Figure 1.6 above). In the non-recombinant asci 4 of the ascospores are recombinant and 4 of the ascospores are non-recombinant (see Figure 1.6 above). To determine how many recombinant and non-recombinant ascospores where in the two pictures above we need to do the math below. You need to use the total number of…
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