Biochemistry: Concepts and Connections (2nd Edition)
Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 15, Problem 8P
Interpretation Introduction

Interpretation:

The 14C label should be traced in 3PG carbon.

Concept introduction:

In green plants the 14CO2 enters with ribulose1,5-biphosphate.

The entry of labeled 14C in the system occurs as carboxylate of one of the molecules of 3-phosphogylcerate.

Hence, the C1- of the phosphoglycerate is first labeled as 14C-carbon. In the cycle, this molecule first gives glyceraldehyde-3-phosphate and then dihydroxyacetone phosphate which further combine to give fructsose-6-biphosphate.

Pictorial representation:

Ribulose-5-phosphate exists in isomeric form, where C-1 is labeled as C-14:

Biochemistry: Concepts and Connections (2nd Edition), Chapter 15, Problem 8P , additional homework tip  1

Biochemistry: Concepts and Connections (2nd Edition), Chapter 15, Problem 8P , additional homework tip  2

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