Human Heredity: Principles and Issues (MindTap Course List)
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN: 9781305251052
Author: Michael Cummings
Publisher: Cengage Learning
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Chapter 15, Problem 16QP
Summary Introduction

To explain: The differences between proteomics and genomics.

Introduction: Proteomics is the study of the set of all proteins that can be encoded by a genome. Genomics is the study of organization and the function and evolution of genomes. However, proteomics is said to be an extension of genomics.

Summary Introduction

To determine: The types of information that can only be provided by proteomics and not by genomics.

Introduction: Proteomics is defined as the study of proteomes. Proteomics identify and characterize all the proteins that are located in a cell, tissue, or organ at a particular time. Genomics is the study of the physiological and evolutionary relationship of genomes.

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Overview of Transformation Protocol   -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes.   Questions: 1) What differences would you expect to see between the…
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Publisher:Cengage Learning